Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GH742_RS07625 Genome accession   NZ_CP045732
Coordinates   1633402..1634622 (-) Length   406 a.a.
NCBI ID   WP_108291623.1    Uniprot ID   A0A3A5LHJ4
Organism   Legionella sp. MW5194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1628402..1639622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH742_RS07600 (GH742_07565) - 1628847..1630292 (+) 1446 WP_203454069.1 bifunctional 2-methylcitrate dehydratase/aconitate hydratase -
  GH742_RS07605 (GH742_07570) - 1630410..1631225 (-) 816 WP_203454071.1 pyruvate, water dikinase regulatory protein -
  GH742_RS07610 (GH742_07575) - 1631393..1631848 (+) 456 WP_203454073.1 hypothetical protein -
  GH742_RS07615 (GH742_07580) - 1631859..1632467 (+) 609 WP_203454075.1 hypothetical protein -
  GH742_RS07620 (GH742_07585) - 1632530..1633387 (-) 858 WP_203454077.1 A24 family peptidase -
  GH742_RS07625 (GH742_07590) pilC 1633402..1634622 (-) 1221 WP_108291623.1 type II secretion system F family protein Machinery gene
  GH742_RS07630 (GH742_07595) pilB 1634628..1636352 (-) 1725 WP_203454079.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GH742_RS07635 (GH742_07600) - 1636541..1637293 (+) 753 WP_203454088.1 class I SAM-dependent methyltransferase -
  GH742_RS07640 (GH742_07605) - 1637363..1637689 (+) 327 WP_108291617.1 DUF1820 family protein -
  GH742_RS07645 (GH742_07610) - 1637768..1638328 (+) 561 WP_203454090.1 hypoxanthine-guanine phosphoribosyltransferase -
  GH742_RS07650 (GH742_07615) - 1638362..1639495 (-) 1134 WP_203454093.1 HlyD family type I secretion periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44855.73 Da        Isoelectric Point: 8.9737

>NTDB_id=396436 GH742_RS07625 WP_108291623.1 1633402..1634622(-) (pilC) [Legionella sp. MW5194]
MEKAKNDIITYQWEGVNKAGEKVNGVIDARSLAIAKADLRKQGIITKKVVKKRKPFFDKKNKKITQGDITVFSRQMATMI
EAGIPLIQSFDIVAKGQSNQKMRDLIETIKRDVETGLTLAESLQKHPAYFNELFCNLVDAGEKSGSLEIMLDKVATYKEK
IETIKKKIKKALTYPMAVLVVAFLVTTALMIFVVPQFESLFEGFGADLPAMTRMVVDMSEFFQSYWYIMFGAIGGAIYAF
IYFKNHSPQFAHSIDRAMLKFPVVGPILEKAAIARFTRTLSITFAAGLPLVEALKSVSGATGNILYAKATDKIREEVSTG
QQMQVAMDNTHLFPNMVIQMVAIGEESGALEQMLGKVADFYEEEVDNAVDALSSLLEPIIMSILGVLVGGLVVAMYLPIF
KLGSAV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=396436 GH742_RS07625 WP_108291623.1 1633402..1634622(-) (pilC) [Legionella sp. MW5194]
ATGGAAAAAGCAAAAAACGACATCATAACCTACCAATGGGAAGGGGTGAATAAAGCCGGCGAGAAGGTGAATGGGGTCAT
TGATGCCCGAAGCCTTGCCATTGCCAAAGCCGACCTTCGCAAACAGGGGATCATTACTAAAAAAGTCGTCAAAAAACGAA
AGCCCTTTTTTGACAAAAAAAATAAAAAAATTACGCAGGGTGATATTACCGTTTTCAGCCGTCAGATGGCGACCATGATT
GAAGCCGGCATTCCCTTAATTCAATCCTTCGATATCGTTGCCAAGGGCCAAAGCAACCAGAAAATGCGTGATCTTATCGA
GACCATTAAACGGGATGTGGAAACTGGCCTGACACTGGCTGAATCACTGCAGAAACACCCCGCGTATTTTAATGAACTCT
TTTGCAACCTGGTTGATGCGGGTGAAAAATCAGGCTCCCTGGAAATCATGCTGGACAAGGTCGCGACTTATAAGGAAAAA
ATTGAAACCATTAAGAAGAAAATCAAGAAAGCCTTAACCTACCCCATGGCTGTATTGGTTGTCGCTTTTTTAGTGACCAC
CGCGCTAATGATATTTGTGGTACCCCAGTTTGAATCCTTGTTTGAAGGATTTGGTGCTGATTTACCCGCCATGACCCGAA
TGGTTGTCGACATGTCTGAATTTTTCCAATCCTATTGGTACATTATGTTCGGCGCCATAGGCGGCGCCATTTATGCCTTT
ATCTATTTTAAAAATCACTCGCCGCAATTCGCCCACTCCATTGATCGGGCCATGTTAAAATTCCCGGTGGTAGGCCCCAT
CCTTGAGAAAGCGGCCATTGCCCGTTTTACCCGAACCCTGTCCATTACCTTTGCAGCCGGTTTGCCTCTGGTTGAAGCTT
TAAAATCGGTGTCCGGAGCAACCGGTAACATCCTGTATGCCAAAGCCACCGACAAGATCAGGGAGGAAGTGTCCACCGGG
CAGCAAATGCAGGTGGCCATGGACAACACACACCTGTTTCCTAATATGGTCATTCAGATGGTGGCTATCGGTGAAGAATC
CGGAGCCCTGGAACAAATGCTGGGTAAAGTGGCGGATTTCTATGAAGAAGAAGTGGATAACGCCGTGGATGCCTTGAGCA
GCCTGCTCGAACCCATTATCATGTCCATTTTAGGGGTTTTGGTCGGCGGCCTGGTGGTGGCCATGTACCTGCCGATTTTC
AAACTGGGTTCAGCGGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A5LHJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

81.773

100

0.818
  pilC Pseudomonas stutzeri DSM 10701

55.721

99.015

0.552
  pilC Acinetobacter baumannii D1279779

56.03

98.03

0.549
  pilC Acinetobacter baylyi ADP1

55.164

97.783

0.539
  pilG Neisseria meningitidis 44/76-A

47.595

97.291

0.463
  pilG Neisseria gonorrhoeae MS11

47.342

97.291

0.461
  pilC Vibrio cholerae strain A1552

41.414

97.537

0.404
  pilC Vibrio campbellii strain DS40M4

40.547

99.015

0.401

Multiple sequence alignment