Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GH742_RS07630 Genome accession   NZ_CP045732
Coordinates   1634628..1636352 (-) Length   574 a.a.
NCBI ID   WP_203454079.1    Uniprot ID   -
Organism   Legionella sp. MW5194     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629628..1641352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GH742_RS07605 (GH742_07570) - 1630410..1631225 (-) 816 WP_203454071.1 pyruvate, water dikinase regulatory protein -
  GH742_RS07610 (GH742_07575) - 1631393..1631848 (+) 456 WP_203454073.1 hypothetical protein -
  GH742_RS07615 (GH742_07580) - 1631859..1632467 (+) 609 WP_203454075.1 hypothetical protein -
  GH742_RS07620 (GH742_07585) - 1632530..1633387 (-) 858 WP_203454077.1 A24 family peptidase -
  GH742_RS07625 (GH742_07590) pilC 1633402..1634622 (-) 1221 WP_108291623.1 type II secretion system F family protein Machinery gene
  GH742_RS07630 (GH742_07595) pilB 1634628..1636352 (-) 1725 WP_203454079.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GH742_RS07635 (GH742_07600) - 1636541..1637293 (+) 753 WP_203454088.1 class I SAM-dependent methyltransferase -
  GH742_RS07640 (GH742_07605) - 1637363..1637689 (+) 327 WP_108291617.1 DUF1820 family protein -
  GH742_RS07645 (GH742_07610) - 1637768..1638328 (+) 561 WP_203454090.1 hypoxanthine-guanine phosphoribosyltransferase -
  GH742_RS07650 (GH742_07615) - 1638362..1639495 (-) 1134 WP_203454093.1 HlyD family type I secretion periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63202.10 Da        Isoelectric Point: 5.3116

>NTDB_id=396437 GH742_RS07630 WP_203454079.1 1634628..1636352(-) (pilB) [Legionella sp. MW5194]
MATAAEEQRLQGIAQLMVHESLLDKDKAIEYQNQAQANKQSLLQYLVSNNLLSAEKIAFTVAQNFGVPLIDLDAIDPDTM
PTSLVNEKLIRRHSIVPLFTRDNHLFLATDDPSKQASLKEIQFHTGLHSNAVVVETNKLNKLIDKLLNEKDSQGLTDYVE
DGAGDLEGLEISADDEEQDTDSSTANDDAPVVKFVNKILVEAIKKGVSDIHFEPYEKEYRIRYRQDGILTEVATPPVSLS
SRITARIKVMSNLDISERRIPQDGRFKMKLSRTRAIDFRVSTCPTVGGEKVVMRILDPGSTKLGIEALGFSSWQRAHFVK
AIEKPQGMILVTGPTGSGKTVSLYTALNILNTIETNISTAEDPVEIKVPGINQVNINPKAGLTFSSTLRSFLRQDPDVIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLINMGIPSFNIASSVSLLIAQRLARRLCEQCKAPRDDFTPQS
LIELGFKEEDAQHVTPYKAVGCNQCNNGYRGRVGLFEVMPMSKAIGQIIMTGGNSLDILKQAQTEGMLTIYQSGLEKIKD
GVTTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=396437 GH742_RS07630 WP_203454079.1 1634628..1636352(-) (pilB) [Legionella sp. MW5194]
ATGGCAACTGCTGCAGAAGAACAACGCTTACAGGGAATTGCTCAGCTGATGGTCCATGAGAGCCTTCTGGACAAGGATAA
AGCTATTGAGTACCAGAATCAGGCTCAGGCGAACAAGCAGTCACTATTGCAGTACCTGGTCTCCAATAACCTGCTTTCTG
CCGAAAAAATTGCGTTCACCGTAGCCCAGAATTTTGGTGTACCCCTGATTGATCTCGATGCGATTGACCCTGATACCATG
CCAACAAGTCTGGTTAACGAGAAATTAATCAGACGCCACTCCATCGTGCCACTTTTTACCCGCGATAATCATCTGTTTCT
GGCAACCGACGATCCAAGCAAACAGGCTTCTCTTAAAGAAATCCAGTTTCACACCGGCCTGCACTCCAATGCGGTAGTCG
TTGAAACCAACAAACTCAATAAATTAATCGACAAATTGCTGAACGAGAAGGATAGCCAGGGCCTGACCGATTATGTGGAG
GATGGCGCCGGCGATCTTGAAGGCCTTGAAATCAGTGCTGACGATGAGGAACAGGATACCGATTCCAGTACGGCAAACGA
TGATGCACCCGTGGTTAAATTTGTTAATAAAATTCTGGTTGAAGCCATTAAAAAAGGCGTGTCGGATATCCATTTTGAAC
CTTATGAAAAAGAATACCGTATCCGCTATCGTCAGGATGGCATTTTGACGGAGGTCGCCACGCCGCCGGTCAGCCTGTCC
AGCCGAATTACTGCCCGGATAAAAGTCATGTCGAATCTTGACATTTCAGAACGTCGTATTCCTCAGGATGGACGCTTTAA
AATGAAGCTTTCACGCACCCGAGCCATTGATTTCCGGGTCAGCACCTGCCCCACCGTCGGTGGCGAAAAAGTGGTGATGC
GTATTCTTGACCCCGGCTCGACAAAACTGGGGATTGAAGCCCTGGGATTCAGCAGCTGGCAACGCGCGCACTTTGTTAAA
GCCATTGAAAAGCCACAAGGCATGATTTTGGTGACTGGCCCGACAGGAAGCGGTAAAACCGTCTCCCTGTACACGGCATT
AAATATCCTGAACACCATAGAGACCAACATTTCCACTGCCGAAGATCCGGTGGAAATTAAAGTACCGGGCATTAACCAGG
TGAATATTAACCCCAAAGCGGGCCTGACCTTCTCCAGCACACTTCGATCTTTCCTTCGCCAGGATCCGGACGTCATCATG
GTCGGGGAGATTCGCGATCTTGAAACCGCAGAAATCGCGGTCAAGGCTGCCCAGACCGGACACCTGGTGCTGTCTACCCT
GCACACCAACAGTGCGGCGGAGACCCTGACCCGTTTGATTAACATGGGCATCCCGTCTTTTAACATCGCAAGCTCGGTAA
GCCTGCTAATCGCCCAGCGTCTCGCAAGGCGTTTATGTGAACAATGCAAAGCCCCGCGTGACGATTTCACGCCGCAAAGT
TTGATTGAGCTGGGTTTTAAAGAAGAGGATGCACAGCACGTCACGCCCTACAAGGCGGTAGGCTGCAATCAATGTAATAA
CGGCTACAGGGGACGGGTGGGCCTTTTTGAAGTCATGCCTATGTCAAAAGCCATCGGTCAAATCATTATGACGGGCGGCA
ACTCACTGGACATCCTGAAACAGGCTCAAACCGAAGGCATGCTGACGATTTATCAATCGGGTCTCGAAAAAATCAAAGAC
GGTGTCACCACCATTGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

77.739

100

0.779
  pilB Acinetobacter baumannii D1279779

54.613

94.425

0.516
  pilB Acinetobacter baylyi ADP1

50.345

100

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

47.163

98.258

0.463
  pilB Vibrio campbellii strain DS40M4

46.631

98.258

0.458
  pilB Vibrio cholerae strain A1552

48.699

93.728

0.456
  pilF Neisseria gonorrhoeae MS11

46.113

98.606

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.073

95.645

0.383
  pilF Thermus thermophilus HB27

37.108

100

0.371

Multiple sequence alignment