Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GFK26_RS33725 Genome accession   NZ_CP045644
Coordinates   7243410..7244639 (+) Length   409 a.a.
NCBI ID   WP_153285790.1    Uniprot ID   A0A5Q0MG40
Organism   Variovorax paradoxus strain 5C-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7238410..7249639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFK26_RS33695 (GFK26_33735) cgtA 7238500..7239576 (-) 1077 WP_153285789.1 Obg family GTPase CgtA -
  GFK26_RS33700 (GFK26_33740) rpmA 7239650..7239910 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  GFK26_RS33705 (GFK26_33745) rplU 7239923..7240234 (-) 312 WP_042578903.1 50S ribosomal protein L21 -
  GFK26_RS33710 (GFK26_33750) - 7240417..7241409 (+) 993 WP_153286198.1 polyprenyl synthetase family protein -
  GFK26_RS33720 (GFK26_33760) pilB 7241652..7243385 (+) 1734 WP_101488972.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GFK26_RS33725 (GFK26_33765) pilC 7243410..7244639 (+) 1230 WP_153285790.1 type II secretion system F family protein Machinery gene
  GFK26_RS33730 (GFK26_33770) - 7244639..7245604 (+) 966 WP_153285791.1 prepilin peptidase -
  GFK26_RS33735 (GFK26_33775) coaE 7245618..7246229 (+) 612 WP_194273996.1 dephospho-CoA kinase -
  GFK26_RS33740 (GFK26_33780) - 7246306..7247613 (+) 1308 WP_228121847.1 MBOAT family O-acyltransferase -
  GFK26_RS33745 (GFK26_33785) - 7247799..7248830 (+) 1032 WP_153280311.1 hypothetical protein -
  GFK26_RS33750 (GFK26_33790) zapD 7248861..7249616 (+) 756 WP_153280312.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44700.41 Da        Isoelectric Point: 9.6713

>NTDB_id=395801 GFK26_RS33725 WP_153285790.1 7243410..7244639(+) (pilC) [Variovorax paradoxus strain 5C-2]
MATVASSRSQVTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKSIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANPSVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEDLLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFSSFGADLPAPTLIVMAISEFFVSYWWLIFGVLGGGI
YFFLQAWKRNERVQKVMDRLLLRLPIFGTLIEKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNSVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=395801 GFK26_RS33725 WP_153285790.1 7243410..7244639(+) (pilC) [Variovorax paradoxus strain 5C-2]
ATGGCAACAGTGGCATCCTCCCGCTCCCAGGTCACGCACAAGGAATTCGTCTTCGAATGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTGCGCGGCGAGCTTCGCGCCGCCGGGGAGAACCAGGTCCAGGCGGCGCTGCGCCGTCAGGGCGTGCTGGCGT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGTCGATCAAGCCCAAGGACATTGCGATCTTCACGCGCCAGCTCGCG
ACCATGATGAAGGCGGGCGTGCCGCTGCTGCAGTCCTTCGACATCGTGGGGCGCGGCAACGCAAATCCGAGTGTGGCCAA
ACTACTGAACGACATCCGTAGCGATGTCGAGACCGGCACCTCGCTGTCGGCCGCGTTCCGCAAGTTTCCGAAGTACTTCG
ACAACCTGTATTGCAACCTGGTGGAAGCCGGTGAGGCCGCCGGTATTCTGGAAGACCTGCTCGATCGCCTGGCGACCTAC
ATGGAGAAGACCGAGGCGATCAAGTCGAAGATCAAGTCGGCGCTGATGTACCCGACCTCGGTGGTCGTCGTGGCCTTCGT
GGTGGTCGCGATCATCATGATCTTCGTGATCCCTGCGTTCAAGCAGGTGTTCTCCTCCTTCGGTGCCGACCTGCCCGCAC
CGACGCTGATCGTGATGGCGATCAGCGAGTTTTTTGTTTCCTACTGGTGGCTGATCTTCGGCGTGCTCGGCGGCGGCATC
TACTTCTTTTTGCAAGCCTGGAAGCGCAACGAGCGCGTCCAGAAGGTCATGGACCGCCTGCTGCTGCGCCTTCCCATCTT
CGGTACGCTGATCGAAAAATCCTGCGTGGCCCGCTGGACCCGCACGCTCGCCACCATGTTCGCAGCCGGCGTTCCGCTGG
TCGAGGCGCTCGATTCCGTGGGCGGCGCATCGGGCAACTCGGTGTACGGAGACGCCACCGCCAAGATCCAGCAGGAAGTC
TCGACCGGCACCAGCCTGACCACGGCCATGACCAACGTCAACCTGTTCCCCTCGATGGTGATCCAGATGACCGCCATCGG
CGAGGAATCGGGCTCCATCGATCACATGCTGGGCAAGGCCGCGGACTTCTACGAGTCCGAAGTCGACGACATGGTCGCCG
GCCTTTCCAGCCTGATGGAGCCCATCATCATCGTGTTCCTCGGCGTCATCATCGGTGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q0MG40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.713

98.778

0.531
  pilC Acinetobacter baylyi ADP1

50.852

100

0.511
  pilG Neisseria gonorrhoeae MS11

52.645

97.066

0.511
  pilG Neisseria meningitidis 44/76-A

52.393

97.066

0.509
  pilC Acinetobacter baumannii D1279779

50.126

97.066

0.487
  pilC Legionella pneumophila strain ERS1305867

50

96.822

0.484
  pilC Vibrio cholerae strain A1552

40.955

97.311

0.399
  pilC Thermus thermophilus HB27

38.425

100

0.394
  pilC Vibrio campbellii strain DS40M4

38.806

98.289

0.381

Multiple sequence alignment