Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GFB63_RS03405 Genome accession   NZ_CP045596
Coordinates   669545..670645 (-) Length   366 a.a.
NCBI ID   WP_144239090.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain TK-P3A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 664545..675645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB63_RS10525 (GFB63_03455) - 664870..665145 (-) 276 WP_084826166.1 CAAX protease -
  GFB63_RS03365 (GFB63_03460) - 665157..665354 (-) 198 WP_084826167.1 helix-turn-helix transcriptional regulator -
  GFB63_RS03370 (GFB63_03465) - 665603..666796 (-) 1194 WP_084826168.1 acetate kinase -
  GFB63_RS03375 (GFB63_03470) comYH 666852..667808 (-) 957 WP_023909974.1 class I SAM-dependent methyltransferase Machinery gene
  GFB63_RS03380 (GFB63_03475) comGG 667853..668170 (-) 318 WP_023909975.1 competence type IV pilus minor pilin ComGG -
  GFB63_RS03385 (GFB63_03480) comYF 668148..668585 (-) 438 WP_084829135.1 competence type IV pilus minor pilin ComGF Machinery gene
  GFB63_RS03390 (GFB63_03485) comGE 668569..668862 (-) 294 WP_084829136.1 competence type IV pilus minor pilin ComGE -
  GFB63_RS03395 (GFB63_03490) comYD 668834..669262 (-) 429 WP_171815055.1 competence type IV pilus minor pilin ComGD Machinery gene
  GFB63_RS03400 (GFB63_03495) comYC 669222..669548 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  GFB63_RS03405 (GFB63_03500) comYB 669545..670645 (-) 1101 WP_144239090.1 competence type IV pilus assembly protein ComGB Machinery gene
  GFB63_RS03410 (GFB63_03505) comYA 670527..671468 (-) 942 WP_014622005.1 competence type IV pilus ATPase ComGA Machinery gene
  GFB63_RS03415 (GFB63_03510) - 671549..671911 (-) 363 WP_023909982.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41839.95 Da        Isoelectric Point: 10.0777

>NTDB_id=395463 GFB63_RS03405 WP_144239090.1 669545..670645(-) (comYB) [Streptococcus thermophilus strain TK-P3A]
MPEKISKTIRRPTGISSWKVWLNKDVSLRGISKGKKLKISQQVKVIQLFKQLLKAGFTLTEIVAFLERSHLLKETSLSLM
KESLIRGDRLDQMLALVGFSDNIVTQISLADKHGNLLGSLTKIETYMLRMTKVRKKLMEVATYPILLLGFLILIMLGLKN
YLLPQLLEGDGKNNWAVQLVQIFPQLFFVSLCGLLVLGLILYLWVKRQSALVFYRRMAKIPFIGQTVRLYTTAYYAREWG
NLVGQGVDLLDLVALMQEQKSKLFRELGADLEEALMLGQSFPERIASYPFFTKELSLIIAYGEANARLGYELEVYAEEVW
QNFFNRLNKATTFVQPLIFVIVAVVIVMIYVAMLLPMYQNMEGMMS

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=395463 GFB63_RS03405 WP_144239090.1 669545..670645(-) (comYB) [Streptococcus thermophilus strain TK-P3A]
TTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAGGTTTGGTTAAACAAGGATGTCTC
ACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAGTCAGCAAGTTAAGGTTATCCAGCTCTTCAAACAACTTTTAA
AGGCCGGTTTTACCTTAACTGAAATCGTAGCCTTTTTGGAGCGAAGTCACTTGCTGAAAGAAACATCCTTGTCTCTTATG
AAAGAGAGTTTAATACGCGGTGATAGGTTGGATCAGATGTTAGCGTTAGTGGGGTTTTCGGACAATATTGTTACTCAGAT
TTCTCTTGCTGACAAGCACGGTAATCTTCTAGGGAGTCTAACAAAGATTGAAACCTATATGCTTCGTATGACAAAGGTTC
GCAAGAAACTCATGGAGGTGGCGACTTATCCCATACTACTTCTGGGTTTTCTGATTCTGATTATGCTAGGACTTAAAAAT
TATCTTCTACCCCAACTCTTAGAGGGTGATGGTAAGAATAATTGGGCTGTACAGTTGGTTCAAATTTTTCCCCAGCTTTT
TTTTGTGAGTTTGTGTGGACTCCTTGTGTTGGGTTTAATTCTCTATCTATGGGTGAAACGACAGTCAGCCCTTGTTTTTT
ATAGGCGAATGGCCAAAATCCCTTTTATTGGTCAGACAGTAAGGCTTTACACGACCGCCTATTATGCTAGGGAATGGGGA
AATTTAGTAGGTCAAGGCGTTGATTTGCTAGATTTGGTGGCTCTTATGCAAGAGCAAAAGTCTAAACTCTTCCGTGAGCT
GGGAGCCGATTTGGAAGAAGCCCTGATGCTGGGACAGAGTTTTCCTGAACGTATTGCCAGTTATCCGTTTTTTACTAAGG
AGCTCTCACTAATTATTGCTTATGGGGAGGCCAATGCGAGGTTGGGCTATGAGTTGGAAGTTTATGCCGAGGAGGTTTGG
CAAAACTTCTTTAACCGTCTTAATAAGGCAACAACCTTTGTGCAACCCCTCATTTTTGTTATTGTTGCAGTTGTGATTGT
AATGATCTATGTAGCCATGCTATTACCAATGTATCAAAATATGGAAGGAATGATGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(236-356)

Type II secretion system (T2SS), protein F (T2SSF)

(51-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.971

93.443

0.514
  comYB Streptococcus mutans UA159

54.971

93.443

0.514
  comYB Streptococcus gordonii str. Challis substr. CH1

51.462

93.443

0.481
  comGB/cglB Streptococcus mitis NCTC 12261

50.755

90.437

0.459
  comGB/cglB Streptococcus mitis SK321

50

90.164

0.451
  comGB/cglB Streptococcus pneumoniae Rx1

50

89.617

0.448
  comGB/cglB Streptococcus pneumoniae D39

50

89.617

0.448
  comGB/cglB Streptococcus pneumoniae R6

50

89.617

0.448
  comGB/cglB Streptococcus pneumoniae TIGR4

50

89.617

0.448
  comGB Lactococcus lactis subsp. cremoris KW2

45.758

90.164

0.413

Multiple sequence alignment