Detailed information NTG0395460

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: GFB63_RS03385
• Genome accession: NZ_CP045596
• Coordinates: 668148..668585 (-)
• Length: 145 a.a.
• NCBI ID: WP_084829135.1
• Uniprot ID: A0A0E2QF53
• Organism: Streptococcus thermophilus strain TK-P3A
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 663148..673585

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFB63_RS03350 (GFB63_03440)	-	663212..663874 (-)	663	WP_084826165.1	type II CAAX endopeptidase family protein	-
GFB63_RS03355 (GFB63_03445)	-	663971..664414 (-)	444	WP_041829335.1	hypothetical protein	-
GFB63_RS10520 (GFB63_03450)	-	664488..664826 (-)	339	WP_228024408.1	CPBP family intramembrane glutamic endopeptidase	-
GFB63_RS10525 (GFB63_03455)	-	664870..665145 (-)	276	WP_084826166.1	CAAX protease	-
GFB63_RS03365 (GFB63_03460)	-	665157..665354 (-)	198	WP_084826167.1	helix-turn-helix transcriptional regulator	-
GFB63_RS03370 (GFB63_03465)	-	665603..666796 (-)	1194	WP_084826168.1	acetate kinase	-
GFB63_RS03375 (GFB63_03470)	comYH	666852..667808 (-)	957	WP_023909974.1	class I SAM-dependent methyltransferase	Machinery gene
GFB63_RS03380 (GFB63_03475)	comGG	667853..668170 (-)	318	WP_023909975.1	competence type IV pilus minor pilin ComGG	-
GFB63_RS03385 (GFB63_03480)	comYF	668148..668585 (-)	438	WP_084829135.1	competence type IV pilus minor pilin ComGF	Machinery gene
GFB63_RS03390 (GFB63_03485)	comGE	668569..668862 (-)	294	WP_084829136.1	competence type IV pilus minor pilin ComGE	-
GFB63_RS03395 (GFB63_03490)	comYD	668834..669262 (-)	429	WP_171815055.1	competence type IV pilus minor pilin ComGD	Machinery gene
GFB63_RS03400 (GFB63_03495)	comYC	669222..669548 (-)	327	WP_002946126.1	competence type IV pilus major pilin ComGC	Machinery gene
GFB63_RS03405 (GFB63_03500)	comYB	669545..670645 (-)	1101	WP_144239090.1	competence type IV pilus assembly protein ComGB	Machinery gene
GFB63_RS03410 (GFB63_03505)	comYA	670527..671468 (-)	942	WP_014622005.1	competence type IV pilus ATPase ComGA	Machinery gene
GFB63_RS03415 (GFB63_03510)	-	671549..671911 (-)	363	WP_023909982.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16329.86 Da
• Isoelectric Point: 8.9855

>NTDB_id=395460 GFB63_RS03385 WP_084829135.1 668148..668585(-) (comYF) [Streptococcus thermophilus strain TK-P3A]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVRQQENQDWLLFVQQMEGELEGCQLVKVEGNKLYVKQD
NQDLAFGLSIASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNGLQRSFIYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=395460 GFB63_RS03385 WP_084829135.1 668148..668585(-) (comYF) [Streptococcus thermophilus strain TK-P3A]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCAGGTAAGACAACAAGAAAACCAAGACTGGCTTCTAT
TTGTTCAACAAATGGAGGGAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAATTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGCTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGGACTAC
AAAGGAGTTTTATTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A0E2QF53

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae D39	50.714	96.552	0.49
comGF/cglF	Streptococcus pneumoniae Rx1	50.714	96.552	0.49
comGF/cglF	Streptococcus pneumoniae R6	50.714	96.552	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50.714	96.552	0.49
comGF/cglF	Streptococcus mitis NCTC 12261	48.227	97.241	0.469
comGF/cglF	Streptococcus mitis SK321	47.518	97.241	0.462
comGF	Lactococcus lactis subsp. cremoris KW2	46.763	95.862	0.448