Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GD578_RS01605 Genome accession   NZ_CP045560
Coordinates   337404..339116 (-) Length   570 a.a.
NCBI ID   WP_002156910.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain AC1530     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 332404..344116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GD578_RS01575 (GD578_01590) - 332568..332894 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  GD578_RS01580 (GD578_01595) rlmB 332996..333745 (+) 750 WP_002156969.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GD578_RS01585 (GD578_01600) - 333781..334695 (+) 915 WP_002156915.1 DMT family transporter -
  GD578_RS01590 (GD578_01605) coaE 334692..335288 (-) 597 WP_002156916.1 dephospho-CoA kinase -
  GD578_RS20355 (GD578_01610) pilD 335290..336150 (-) 861 WP_002157022.1 A24 family peptidase Machinery gene
  GD578_RS01600 (GD578_01615) pilC 336150..337376 (-) 1227 WP_002156990.1 type II secretion system F family protein Machinery gene
  GD578_RS01605 (GD578_01620) pilB 337404..339116 (-) 1713 WP_002156910.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GD578_RS01610 (GD578_01625) tpiA 339407..340201 (+) 795 WP_002156912.1 triose-phosphate isomerase -
  GD578_RS01615 (GD578_01630) secG 340214..340543 (+) 330 WP_002156913.1 preprotein translocase subunit SecG -
  GD578_RS01635 (GD578_01650) rimP 341180..341704 (+) 525 WP_002157009.1 ribosome maturation factor RimP -
  GD578_RS01640 (GD578_01655) nusA 341742..343226 (+) 1485 WP_002156952.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63489.91 Da        Isoelectric Point: 5.2413

>NTDB_id=395126 GD578_RS01605 WP_002156910.1 337404..339116(-) (pilB) [Acinetobacter nosocomialis strain AC1530]
MSVLHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPMGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=395126 GD578_RS01605 WP_002156910.1 337404..339116(-) (pilB) [Acinetobacter nosocomialis strain AC1530]
ATGTCAGTATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCCAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACCATTTCTGCGGAGTTTGCTGAACCATATTTTGATCTAGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTCTATATGT
CGCGACCAGCAACCCAAGCAATATTGAAGCAATCGATGCAATCCGTTTTAACAGTAAATTGCTGGTTGAACCTGTTATTG
TCGAGCATCATAAGCTGGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTTAATGATGAAGAGTTT
GATCTTGATGTCAGCCTTGATGGTCCTACCACTCAAGAAGATGAAGAAGAAACACCTCAAGGTGATGAAGCTCCTATTGT
TAAATATATTAATAAGTTGCTTATTGATGCCATTCGTATGGGCGCGTCAGATTTACATTTTGAGCCTTATGAAAAGTCAT
ATCGGGTACGTTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCGCCTTTACAACTTGCAAACCGTTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGACGTATTAAACTTAAGCTGTCAAA
AACCAAAGCAATTGATTTCCGTGTGAACTCGCTCCCTACCTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGCATTGATGCATTAGGCTACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTGATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAGAGTTCCAATATTTCTACTGCCGAAGATCCAGTGGAAATTAACTTAGAAGGTATTAATCAGGTTAACGTCAATCCTA
AAGTGGGTTTAACGTTTGCAGCTGCACTTAAGTCTTTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCTGCTCAAACCGGCCATATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAATCTAGTGATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGTAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACATCCAGATTTTCGGGTTTTTCAACCCATGGGCTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

98.421

100

0.984
  pilB Acinetobacter baylyi ADP1

79.649

100

0.796
  pilB Legionella pneumophila strain ERS1305867

50.617

99.474

0.504
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.189

98.596

0.367

Multiple sequence alignment