Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GEV41_RS26545 Genome accession   NZ_CP045551
Coordinates   5744416..5745678 (-) Length   420 a.a.
NCBI ID   WP_019752678.1    Uniprot ID   -
Organism   Pseudomonas putida strain NY5709     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5739416..5750678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GEV41_RS26530 (GEV41_27315) - 5741082..5742683 (-) 1602 WP_003249151.1 SPOR domain-containing protein -
  GEV41_RS26535 (GEV41_27320) aroB 5742699..5743796 (-) 1098 WP_003249149.1 3-dehydroquinate synthase -
  GEV41_RS26540 (GEV41_27325) aroK 5743893..5744411 (-) 519 WP_003249147.1 shikimate kinase AroK -
  GEV41_RS26545 (GEV41_27330) pilQ 5744416..5745678 (-) 1263 WP_019752678.1 type IV pilus secretin PilQ Machinery gene
  GEV41_RS26550 (GEV41_27335) - 5745602..5746603 (-) 1002 WP_173881452.1 pilus assembly protein PilP -
  GEV41_RS26555 (GEV41_27340) - 5746615..5747151 (-) 537 WP_173881259.1 PilN domain-containing protein -
  GEV41_RS26560 (GEV41_27345) pilM 5747148..5748029 (-) 882 WP_098089510.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45688.38 Da        Isoelectric Point: 4.9839

>NTDB_id=395005 GEV41_RS26545 WP_019752678.1 5744416..5745678(-) (pilQ) [Pseudomonas putida strain NY5709]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQVLDAQLQPLRRELLTIHHAKAADLAQLLLASLEGGGISTGSLSVDERT
NTLVVYLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=395005 GEV41_RS26545 WP_019752678.1 5744416..5745678(-) (pilQ) [Pseudomonas putida strain NY5709]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTCG
CCAGTGATGCGGTACAAGGCAGCGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTCGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGCCAAGTGCTGGACGCGCAGCTGCAGCCATTGCGTCGGGAGCTGCTGACCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCTGGCAAGTCTGGAGGGTGGCGGCATCTCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGTTGGTGGCGCAGTTGGACGTGCCGGTGCG
TCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAGCGAGCCGGCAGCAGTATCGGCCTG
GGTCTGTTGCGCGGTGACGTGTTGCTGGATCTGGAACTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACCAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACTCCGGACAACAAGGTG
ATCATGACCGTCCGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(126-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.617
  comQ Acinetobacter baylyi ADP1

42.889

100

0.46
  pilQ Acinetobacter baumannii strain A118

40.998

100

0.45
  pilQ Acinetobacter baumannii D1279779

40.998

100

0.45
  pilQ Legionella pneumophila strain ERS1305867

43.269

99.048

0.429
  pilQ Legionella pneumophila strain Lp02

43.269

99.048

0.429
  pilQ Vibrio campbellii strain DS40M4

41.014

100

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

98.81

0.419
  pilQ Vibrio cholerae strain A1552

42.41

98.81

0.419

Multiple sequence alignment