Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GAY96_RS10495 Genome accession   NZ_CP045550
Coordinates   2221727..2223427 (+) Length   566 a.a.
NCBI ID   WP_228344961.1    Uniprot ID   -
Organism   Venatoribacter cucullus strain ASxS5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2216727..2228427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GAY96_RS10475 (GAY96_10415) - 2217387..2218916 (+) 1530 WP_265573462.1 O-antigen ligase family protein -
  GAY96_RS10485 (GAY96_10420) - 2219136..2219585 (+) 450 WP_277613408.1 pilin -
  GAY96_RS10490 (GAY96_10425) - 2219647..2221650 (+) 2004 WP_265573463.1 sulfatase-like hydrolase/transferase -
  GAY96_RS10495 (GAY96_10430) pilB 2221727..2223427 (+) 1701 WP_228344961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GAY96_RS10500 (GAY96_10435) pilC 2223473..2224699 (+) 1227 WP_265573464.1 type II secretion system F family protein Machinery gene
  GAY96_RS10505 (GAY96_10440) pilD 2224699..2225577 (+) 879 WP_265573465.1 prepilin peptidase Machinery gene
  GAY96_RS10510 (GAY96_10445) coaE 2225577..2226173 (+) 597 WP_420910981.1 dephospho-CoA kinase -
  GAY96_RS10515 (GAY96_10450) yacG 2226304..2226504 (+) 201 WP_265573466.1 DNA gyrase inhibitor YacG -
  GAY96_RS10520 (GAY96_10455) - 2226640..2227098 (-) 459 WP_265573467.1 copper chaperone PCu(A)C -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 61781.75 Da        Isoelectric Point: 5.0195

>NTDB_id=394975 GAY96_RS10495 WP_228344961.1 2221727..2223427(+) (pilB) [Venatoribacter cucullus strain ASxS5]
MAVLTGLPRRLVEQNLITSQQAQQVLDKSRQDKTSFVTAAVASGQVKARPVALLASEEFGVPLLDLTAFDFEVMPKELVS
RELIEKHRVLPLFQRGNRLYIAQSDPSAMLAIDEIKFATGMTIEAILVEEDKLSHAVDKFLAANDNSLGDLGGDFEDVDL
EDPEARSKEQEADSKEGTGDDAPIVRFVNKMLLDAIKAGSSDIHFEPYEKRYRVRYRTDGVLHEVSSPPVNLAGKIAARL
KVMSQMDISERRIPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDMLGYEPEQKALYMEALARPQG
MILVTGPTGSGKTVSLYTGLNILNVEGTNISTAEDPVEINLEGINQVNVNARVGLDFAAALRSFLRQDPDVVMVGEIRDL
DTASIAIKAAQTGHLVLSTLHTNSAAETLTRLANMGVPSFNIATSVSLIIAQRLARRLCSACKVPAEIPKETLLEAGFTE
AQLQDAEIYKPVGCEQCKGGYKGRVGIYEVVKITDSISRIIMEGGNSIEISDAARKEGFNDLRTSALMKVAQGVTSLEEA
NRVTTD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=394975 GAY96_RS10495 WP_228344961.1 2221727..2223427(+) (pilB) [Venatoribacter cucullus strain ASxS5]
ATGGCCGTGCTGACCGGTTTGCCACGCCGTCTTGTCGAACAAAATCTGATCACCAGCCAGCAGGCACAGCAGGTACTGGA
TAAGTCCCGGCAGGATAAAACCAGCTTTGTAACTGCTGCCGTCGCCTCCGGGCAGGTGAAAGCGCGCCCGGTGGCGCTGC
TGGCCTCGGAAGAGTTTGGTGTACCGCTGCTGGATCTGACCGCCTTCGATTTTGAAGTAATGCCCAAAGAGCTGGTCAGC
CGTGAATTAATTGAAAAGCACCGTGTGTTGCCGCTGTTCCAGCGTGGCAACCGGCTGTACATCGCCCAGAGCGACCCCTC
GGCCATGCTGGCCATTGATGAAATCAAATTCGCCACCGGCATGACCATCGAAGCCATTCTGGTGGAAGAAGATAAATTGT
CCCACGCGGTGGATAAATTCCTGGCCGCCAACGACAACAGCCTGGGGGATTTGGGCGGCGACTTCGAAGATGTCGACCTG
GAAGACCCGGAAGCCCGCAGTAAAGAACAGGAAGCCGACAGCAAAGAAGGCACTGGCGATGACGCACCGATTGTGCGCTT
TGTGAACAAAATGCTGCTGGATGCTATTAAAGCCGGCTCGTCCGATATTCACTTTGAACCCTACGAAAAACGCTACCGCG
TGCGGTACCGTACCGATGGTGTATTGCACGAAGTCTCCAGCCCGCCGGTGAATCTGGCCGGCAAAATTGCTGCCCGTCTG
AAAGTGATGTCGCAAATGGATATTTCCGAGCGGCGGATTCCGCAGGACGGCCGCATCAAGATGAAGCTGTCCAAATCCAA
GGCCATCGACTTCCGGGTAAACACCTTGCCCACTCTGTGGGGCGAAAAAATCGTACTGCGGATTCTGGACCCGTCGTCGG
CCAAACTGGGCATTGATATGCTGGGTTACGAGCCGGAACAGAAAGCCCTGTATATGGAAGCTCTGGCGCGTCCGCAGGGT
ATGATTCTGGTTACCGGCCCCACCGGGTCTGGTAAAACCGTATCGCTGTACACCGGCCTGAATATTCTGAACGTCGAAGG
CACCAATATTTCCACCGCCGAAGACCCGGTGGAAATTAACCTGGAAGGTATTAACCAGGTGAACGTTAACGCCCGCGTGG
GGCTGGATTTCGCCGCGGCCCTGCGTTCGTTCCTGCGTCAGGACCCGGATGTGGTGATGGTGGGTGAGATCCGTGACCTG
GATACCGCTTCCATTGCCATTAAAGCGGCCCAGACCGGTCACTTAGTGCTGTCCACCCTGCACACCAACAGCGCCGCCGA
AACCTTAACGCGTCTGGCCAACATGGGCGTACCGTCCTTTAACATTGCCACCTCGGTATCGTTGATCATTGCCCAGCGTC
TGGCCCGGCGTTTGTGCTCAGCCTGTAAAGTACCGGCTGAAATCCCCAAGGAAACGCTGCTGGAAGCCGGTTTTACCGAA
GCCCAGCTGCAGGATGCCGAAATTTATAAGCCGGTGGGCTGCGAACAATGCAAAGGCGGTTACAAAGGCCGTGTTGGTAT
TTATGAAGTAGTTAAAATTACCGACAGCATCTCCCGTATTATTATGGAGGGCGGCAACTCCATCGAAATTTCGGACGCTG
CCCGCAAAGAAGGCTTTAACGATCTGCGCACCTCGGCCCTGATGAAAGTGGCACAAGGGGTAACCAGCCTGGAAGAAGCC
AACCGCGTAACCACCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.721

100

0.627
  pilB Acinetobacter baylyi ADP1

60.915

100

0.611
  pilB Legionella pneumophila strain ERS1305867

55.398

99.823

0.553
  pilB Vibrio cholerae strain A1552

50.709

99.647

0.505
  pilF Neisseria gonorrhoeae MS11

50.353

100

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.538

98.587

0.498
  pilB Vibrio campbellii strain DS40M4

49.117

100

0.491
  pilB/pilB1 Synechocystis sp. PCC 6803

38.681

99.117

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.662

93.993

0.373
  pilF Thermus thermophilus HB27

36.778

100

0.371

Multiple sequence alignment