Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GAY96_RS10505 Genome accession   NZ_CP045550
Coordinates   2224699..2225577 (+) Length   292 a.a.
NCBI ID   WP_265573465.1    Uniprot ID   -
Organism   Venatoribacter cucullus strain ASxS5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2219699..2230577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GAY96_RS10495 (GAY96_10430) pilB 2221727..2223427 (+) 1701 WP_228344961.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GAY96_RS10500 (GAY96_10435) pilC 2223473..2224699 (+) 1227 WP_265573464.1 type II secretion system F family protein Machinery gene
  GAY96_RS10505 (GAY96_10440) pilD 2224699..2225577 (+) 879 WP_265573465.1 prepilin peptidase Machinery gene
  GAY96_RS10510 (GAY96_10445) coaE 2225577..2226173 (+) 597 WP_420910981.1 dephospho-CoA kinase -
  GAY96_RS10515 (GAY96_10450) yacG 2226304..2226504 (+) 201 WP_265573466.1 DNA gyrase inhibitor YacG -
  GAY96_RS10520 (GAY96_10455) - 2226640..2227098 (-) 459 WP_265573467.1 copper chaperone PCu(A)C -
  GAY96_RS10525 (GAY96_10460) - 2227240..2229201 (-) 1962 WP_265573468.1 bifunctional diguanylate cyclase/phosphodiesterase -
  GAY96_RS10530 (GAY96_10465) - 2229253..2229921 (-) 669 WP_265573469.1 transglutaminase-like cysteine peptidase -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 32000.13 Da        Isoelectric Point: 8.0462

>NTDB_id=394977 GAY96_RS10505 WP_265573465.1 2224699..2225577(+) (pilD) [Venatoribacter cucullus strain ASxS5]
MSTLAFIGNEPWLAALSLVVLGLLIGSFLNVVILRFPIMLFRGWKRDCEEMVSTLPDHPALANMDKPFNLLKPDSHCPKC
HAPVRAWQNIPVISWLLLRGKCASCGTRIGWRYPAVELATALFSAVLLLVFPWGWQLAAMLVFTWLLISMAVIDIDHQIL
PDTMTLGLMWLGLAINTQGLFTDLTSAVWGAIGGYGLLWSVFWLFKLATGKEGMGYGDFKLLAALGAWLGVQSLPLIILL
SSVVGAVVGIAGILLQGRDKNVPIPFGPYLAAAGWIAALWGNDITRWYVGHF

Nucleotide


Download         Length: 879 bp        

>NTDB_id=394977 GAY96_RS10505 WP_265573465.1 2224699..2225577(+) (pilD) [Venatoribacter cucullus strain ASxS5]
ATGTCCACCCTCGCATTTATCGGCAACGAACCCTGGCTGGCTGCACTCAGCCTGGTGGTACTGGGCCTGCTGATCGGCAG
TTTTCTGAATGTGGTGATTCTGCGCTTTCCCATCATGCTGTTCCGGGGCTGGAAGCGCGATTGTGAAGAAATGGTCAGCA
CTTTGCCCGACCATCCGGCGCTGGCCAATATGGATAAGCCGTTTAACCTGCTGAAACCCGATTCCCATTGCCCGAAATGC
CACGCGCCGGTACGAGCCTGGCAGAATATTCCGGTCATCAGCTGGCTGCTGTTGCGCGGTAAATGTGCCAGCTGCGGCAC
CCGCATTGGCTGGCGTTACCCGGCGGTGGAACTGGCCACCGCGCTGTTCAGTGCGGTATTGCTGCTGGTCTTTCCCTGGG
GCTGGCAACTGGCCGCCATGCTGGTATTTACCTGGCTGTTAATCAGCATGGCGGTGATTGATATTGACCACCAGATTCTG
CCCGACACCATGACGCTGGGCCTGATGTGGCTGGGGCTGGCCATTAACACGCAGGGCCTGTTTACCGATTTAACCAGCGC
CGTCTGGGGTGCCATTGGGGGTTACGGTTTACTGTGGTCGGTGTTCTGGTTATTCAAACTGGCCACCGGCAAAGAAGGCA
TGGGCTACGGCGACTTCAAGCTTCTGGCCGCGCTGGGCGCCTGGCTGGGTGTACAAAGCCTGCCACTGATTATTCTGCTG
TCGTCCGTCGTGGGTGCGGTGGTGGGAATTGCCGGCATTCTGCTGCAGGGCCGCGATAAAAACGTCCCCATTCCCTTTGG
TCCGTATCTGGCCGCCGCCGGCTGGATTGCAGCACTGTGGGGCAACGACATTACCCGCTGGTACGTCGGACACTTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(141-250)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

52

94.178

0.49
  pilD Acinetobacter baumannii D1279779

52

94.178

0.49
  pilD Vibrio campbellii strain DS40M4

49.104

95.548

0.469
  pilD Vibrio cholerae strain A1552

48.746

95.548

0.466
  pilD Neisseria gonorrhoeae MS11

49.091

94.178

0.462

Multiple sequence alignment