Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HIBPF_RS01225 Genome accession   NC_014920
Coordinates   252028..252477 (-) Length   149 a.a.
NCBI ID   WP_006996184.1    Uniprot ID   A0AAV2U2D0
Organism   Haemophilus influenzae F3031     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 247028..257477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIBPF_RS01205 (HIBPF02400) rho 247417..248679 (+) 1263 WP_005666690.1 transcription termination factor Rho -
  HIBPF_RS01210 (HIBPF02410) pilD 248734..249426 (-) 693 WP_013525531.1 prepilin peptidase Machinery gene
  HIBPF_RS01215 (HIBPF02420) pilC 249423..250640 (-) 1218 WP_013525532.1 type II secretion system F family protein Machinery gene
  HIBPF_RS01220 (HIBPF02430) pilB 250637..252031 (-) 1395 WP_013525533.1 GspE/PulE family protein Machinery gene
  HIBPF_RS01225 (HIBPF02431) pilA 252028..252477 (-) 450 WP_006996184.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HIBPF_RS01230 (HIBPFp01) ampD 252592..253152 (+) 561 WP_006996183.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HIBPF_RS01235 (HIBPF02460) corC 253775..254674 (+) 900 WP_013525534.1 CNNM family magnesium/cobalt transport protein CorC -
  HIBPF_RS01240 (HIBPF02470) lnt 254697..256226 (+) 1530 WP_013525535.1 apolipoprotein N-acyltransferase -
  HIBPF_RS01245 (HIBPF02480) rsmE 256276..257013 (+) 738 WP_006996180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15542.86 Da        Isoelectric Point: 8.0922

>NTDB_id=39404 HIBPF_RS01225 WP_006996184.1 252028..252477(-) (pilA) [Haemophilus influenzae F3031]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITAAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=39404 HIBPF_RS01225 WP_006996184.1 252028..252477(-) (pilA) [Haemophilus influenzae F3031]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCGCAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTTACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

99.329

100

0.993
  pilA Haemophilus influenzae Rd KW20

91.275

100

0.913
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

42.177

98.658

0.416
  pilA2 Legionella pneumophila strain ERS1305867

42.177

98.658

0.416
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376

Multiple sequence alignment