Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FIU84_RS02725 Genome accession   NZ_CP045416
Coordinates   594438..596594 (+) Length   718 a.a.
NCBI ID   WP_063539616.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain THAF7b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589438..601594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU84_RS02705 (FIU84_02720) comM 591604..592668 (+) 1065 WP_063539612.1 pilus assembly protein PilM Machinery gene
  FIU84_RS02710 (FIU84_02725) - 592668..593240 (+) 573 WP_063539613.1 PilN domain-containing protein -
  FIU84_RS02715 (FIU84_02730) pilO 593237..593860 (+) 624 WP_063539614.1 type 4a pilus biogenesis protein PilO -
  FIU84_RS02720 (FIU84_02735) pilP 593857..594384 (+) 528 WP_063539615.1 type 4a pilus biogenesis lipoprotein PilP -
  FIU84_RS02725 (FIU84_02740) pilQ 594438..596594 (+) 2157 WP_063539616.1 type IV pilus secretin PilQ Machinery gene
  FIU84_RS02730 (FIU84_02745) aroK 596600..597145 (+) 546 WP_063539617.1 shikimate kinase AroK -
  FIU84_RS02735 (FIU84_02750) aroB 597168..598271 (+) 1104 WP_063539618.1 3-dehydroquinate synthase -
  FIU84_RS02740 (FIU84_02755) - 598282..599847 (+) 1566 WP_063539619.1 AAA family ATPase -

Sequence

Protein


Download         Length: 718 a.a.        Molecular weight: 77230.56 Da        Isoelectric Point: 4.8056

>NTDB_id=393846 FIU84_RS02725 WP_063539616.1 594438..596594(+) (pilQ) [Stutzerimonas frequens strain THAF7b]
MNSTLSRLGISVLVAFVSPAVLAANLNALDVAALPGDRVELKLAFDEPVAPPRGYTLEQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGKNLFVVLGENAGVNGPASVSAQPSAVAQPSVTTAPAKAVAVGKA
IDNIDFRRGDDGAGNVIITLSDPSISSTIEEQGGKIRVLFDKAQLPEALRVRLDVQDFATPVQFVDSSAQGKGASVIIQP
TGRYDYLAYQTDNKLTISIKPLTEDEAEKRQSERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRL
QNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTNN
AASAEDRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLWAGDKWNAWG
KNGQLGVADDPDASGKPGEDGYIPSRQVGRFLGIPEVPMNTPFVDMGAVGATSGIGLGFITNNAILDLQLSAMEKTGNGE
VVSQPKVVTADKETAKILKGAEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAATTGVPA
IRKNEVNAKVLVTDGETIVIGGVFSNTQSKSVDKVPLLGDIPFVGRLFRRDLVQDRKSELLVFITPRIMNNQAIQVSR

Nucleotide


Download         Length: 2157 bp        

>NTDB_id=393846 FIU84_RS02725 WP_063539616.1 594438..596594(+) (pilQ) [Stutzerimonas frequens strain THAF7b]
ATGAACAGTACTCTCTCGCGCCTCGGCATTTCAGTGCTTGTGGCGTTCGTCTCGCCGGCTGTGCTGGCAGCCAATCTCAA
TGCCTTGGATGTGGCCGCTCTGCCGGGCGATCGAGTCGAGCTCAAGCTGGCTTTCGACGAGCCCGTTGCGCCGCCGCGTG
GTTACACCCTTGAACAGCCTGCTCGCATTGCCCTGGATCTGCCGGGCGTATCGAACAATCTGGGAGCGAAGAATCGTGAG
TTGGGGGTTGGTAATGCGCGTAGCGTTACGGTCGTTGAGGCGCAAGGACGTACTCGGTTGATCGTAAACCTCACTTCGCT
GGTGCCCTATTCCACTCGAGTGGATGGCAAGAATCTGTTCGTCGTGCTGGGCGAAAATGCCGGCGTGAATGGCCCGGCAT
CGGTCTCAGCGCAGCCGAGCGCAGTGGCGCAGCCGTCTGTTACGACGGCTCCCGCGAAGGCGGTGGCTGTTGGCAAGGCC
ATTGACAATATTGACTTTCGGCGTGGCGATGATGGAGCCGGTAACGTGATTATTACGCTATCCGATCCATCAATCAGCTC
GACCATTGAGGAGCAGGGCGGAAAGATCCGTGTCCTTTTCGACAAAGCTCAACTGCCAGAAGCACTGCGTGTCCGTCTTG
ATGTTCAGGACTTTGCTACTCCCGTGCAGTTTGTGGATTCTTCCGCTCAGGGAAAAGGTGCGAGTGTGATCATTCAGCCG
ACTGGTCGGTATGACTATTTGGCCTATCAAACTGACAACAAACTCACCATTAGCATCAAGCCGCTGACAGAGGATGAGGC
GGAGAAGCGTCAGTCGGAGCGTTTTGCTTATTCAGGCGAGAAGTTGTCGCTCAACTTTCAAGATATCGACGTTCGTTCCG
TACTGCAGTTGATCGCGGACTTCACCGATCTGAATCTGGTGGCGAGCGATACTGTAGCCGGCAACATAACCCTGCGGCTG
CAAAACGTGCCTTGGGACCAGGCGCTTGATCTGGTGCTCAAAACCAAAGGTCTTGATAAACGTCAGGTTGGTAATGTCTT
GTTGGTCGCGCCTGCTGACGAAATTGCTGCACGCGAACGACAGGAGTTAGAGTCGCAACGGCAGATCGCCGAGCTTGCTC
CGCTGCGTCGCGAGGTGGTTCAGGTCAATTATGCTAAAGCTGCCGATATTGCTCGGTTGTTTCAGTCAGTTACCAATAAT
GCTGCATCGGCAGAAGATCGTGGGTCTATTGCTGTCGATGATCGGACAAACAATATTATCGCTTATCAGACACAGGAACG
ACTGGATGAGCTGCGCCGTATAGTCGCTCAGTTAGATATTCCGGTACGTCAGGTGATGATTGAGGCCCGGATTGTGGAAG
CTAACGTCGACTACGATAAAAGCTTAGGGGTTCGCTGGGGAGGGAATCTTTGGGCTGGGGATAAGTGGAATGCTTGGGGA
AAGAACGGACAGCTCGGTGTTGCTGACGATCCTGATGCGAGCGGCAAGCCCGGAGAAGATGGCTACATCCCCTCTCGTCA
GGTTGGGCGTTTCCTAGGGATACCTGAAGTACCAATGAACACTCCCTTTGTCGACATGGGGGCGGTTGGTGCGACATCTG
GCATAGGGTTGGGGTTTATTACTAATAACGCTATTTTGGATCTTCAGCTTTCTGCGATGGAGAAAACTGGAAATGGCGAG
GTTGTCTCTCAGCCCAAGGTTGTCACGGCAGACAAAGAAACTGCGAAAATTTTGAAGGGTGCTGAAGTACCTTATCAGGA
GGCCAGCTCGAGCGGTGCCACCAGCACTTCGTTCAAGGAGGCAGCACTTTCGTTGGAGGTTACACCGCAGATTACCCCGG
ATAATCGAATTATCATGGAGGTCAAGGTGACCAAGGATGAGCCTGATTTTTCACAAGCTGCTACGACTGGTGTTCCAGCC
ATTCGTAAGAATGAAGTTAATGCGAAGGTTCTAGTGACTGATGGCGAAACGATTGTTATCGGCGGTGTCTTTTCCAATAC
GCAGAGCAAATCTGTCGATAAGGTGCCTTTGCTAGGTGATATTCCTTTTGTCGGTCGACTGTTCCGCCGTGACCTGGTTC
AGGACCGAAAGTCGGAGCTGCTTGTGTTCATCACTCCGCGAATCATGAATAATCAGGCGATTCAAGTCAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)

AMIN domain (AMIN)

(38-123)

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II and III secretion system protein (Secretin)

(552-709)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.995

100

0.734
  comQ Acinetobacter baylyi ADP1

41.201

99.721

0.411
  pilQ Acinetobacter baumannii strain A118

38.889

100

0.409
  pilQ Acinetobacter baumannii D1279779

38.889

100

0.409
  pilQ Legionella pneumophila strain ERS1305867

38.116

96.1

0.366
  pilQ Legionella pneumophila strain Lp02

37.971

96.1

0.365

Multiple sequence alignment