Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FIU96_RS03235 Genome accession   NZ_CP045367
Coordinates   702258..704372 (+) Length   704 a.a.
NCBI ID   WP_152437318.1    Uniprot ID   -
Organism   Marinobacter sp. THAF39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697258..709372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU96_RS03215 (FIU96_03260) pilM 699456..700520 (+) 1065 WP_152437317.1 pilus assembly protein PilM Machinery gene
  FIU96_RS03220 (FIU96_03265) - 700520..701083 (+) 564 WP_106693178.1 PilN domain-containing protein -
  FIU96_RS03225 (FIU96_03270) - 701083..701694 (+) 612 WP_072678328.1 type 4a pilus biogenesis protein PilO -
  FIU96_RS03230 (FIU96_03275) - 701697..702239 (+) 543 WP_061332384.1 pilus assembly protein PilP -
  FIU96_RS03235 (FIU96_03280) pilQ 702258..704372 (+) 2115 WP_152437318.1 type IV pilus secretin PilQ Machinery gene
  FIU96_RS03240 (FIU96_03285) aroK 704496..705080 (+) 585 WP_138438137.1 shikimate kinase AroK -
  FIU96_RS03245 (FIU96_03290) aroB 705055..706143 (+) 1089 WP_152437319.1 3-dehydroquinate synthase -
  FIU96_RS03250 (FIU96_03295) - 706187..707668 (+) 1482 WP_152437320.1 AAA family ATPase -

Sequence

Protein


Download         Length: 704 a.a.        Molecular weight: 75851.53 Da        Isoelectric Point: 4.4783

>NTDB_id=393672 FIU96_RS03235 WP_152437318.1 702258..704372(+) (pilQ) [Marinobacter sp. THAF39]
MNEKNMHEQNGLRSRLAMFKKLNVCVGVIAIGLWTSLASAVTLEDVSFSSLPGERLEVTMKFDGPPPEPTGYTIERPARI
AVDLKDTTSGLNQRSIPLGSGNAQSVTVVETKDRTRLIFNLVELVPHDTVRQNNSLVMTIGGASGAPTVTAGAQQSRTGT
TNASAANALAGVDFRRSKEGEGRVIVDLGSASAPVNLSELAGRIRLTMPGLEVPVDLRRRLDVTDFATPVTRIDTFIEDG
NAVVEIRPEGNYDYIAYQSGREFTVSVERISEEEAETRREEKFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTV
GGSITLRLQNVPWDQALDLILKTKGLDQRQIGNVLLVAPAEEIAAREKLELETSKQIAELAPVRLDIIQVNYAKAADVVA
LIRADEELISSRGFVSSDVRTNTISVRETQQKLEEIRRLVGTWDVPVRQVSIEARIVRAQTNVAENLGVRWGGAAYDVSG
NDVISIGGSLGTLQEARDAAAGGGGGISFPGALGVDLGVSGDGASSFAIGWGSDDFLIDLELSALETDGRAEVVSQPRVV
TADRQSASIKSGEEIPYQEATSSGATNVEFKEAVLSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPSINTNSVTTQVL
VANGETVVLGGIFQSEVATQTTKTPFLGDIPYLGRLFKRTEHIDERSELLIFITPKIIKNDLIQ

Nucleotide


Download         Length: 2115 bp        

>NTDB_id=393672 FIU96_RS03235 WP_152437318.1 702258..704372(+) (pilQ) [Marinobacter sp. THAF39]
ATGAACGAAAAAAACATGCACGAACAAAATGGTTTAAGGTCGAGGCTAGCGATGTTCAAAAAACTCAATGTCTGCGTGGG
CGTGATCGCCATCGGGTTATGGACCAGCCTGGCCAGCGCGGTCACGCTGGAAGACGTGTCGTTTTCGTCGCTGCCGGGAG
AACGCCTGGAAGTGACCATGAAATTCGACGGGCCACCGCCGGAGCCAACCGGTTACACGATTGAGCGCCCGGCGCGCATC
GCCGTGGACCTCAAAGACACCACCAGTGGGCTTAACCAGCGGAGCATTCCGCTTGGATCTGGCAACGCCCAGAGCGTGAC
TGTGGTGGAAACCAAAGATCGCACACGGCTGATCTTTAACCTGGTCGAGCTGGTGCCGCACGATACCGTTCGCCAGAACA
ATTCGTTGGTGATGACCATTGGTGGCGCCAGTGGCGCGCCGACGGTGACAGCAGGAGCCCAGCAGAGCAGGACCGGAACA
ACCAATGCCTCTGCAGCCAACGCCTTGGCTGGCGTTGACTTCCGTCGCAGCAAAGAGGGTGAAGGGCGCGTTATTGTTGA
TCTGGGTAGTGCGTCGGCTCCGGTGAACCTGTCTGAACTGGCCGGTCGCATTCGCCTGACCATGCCGGGCCTTGAAGTGC
CTGTGGATCTCCGTCGCCGTCTCGACGTGACTGACTTTGCGACCCCTGTTACCCGCATTGATACCTTTATTGAGGATGGT
AACGCTGTTGTTGAGATTCGCCCGGAAGGAAACTACGACTACATAGCGTATCAGTCTGGCCGTGAATTCACCGTGAGCGT
GGAACGCATCTCGGAAGAAGAGGCAGAGACCCGTCGCGAAGAGAAATTCCCGTACACCGGTGACAAGCTGTCCTTGAACT
TCCAGGACATTGAAGTACGTTCGGTTCTCCAGTTGATTGCCGACTTTACCGGGCTGAACCTGGTGGCCAGTGATACCGTT
GGTGGCAGCATTACCCTGCGTTTGCAGAATGTACCCTGGGATCAGGCGCTTGATCTGATTTTGAAGACCAAAGGTCTGGA
CCAGCGTCAGATCGGCAACGTTCTGCTGGTGGCTCCGGCAGAGGAAATCGCCGCCCGTGAAAAGCTTGAGCTCGAGACCA
GTAAGCAAATTGCGGAACTGGCCCCAGTGCGCCTTGATATCATTCAGGTGAACTACGCGAAGGCGGCCGATGTTGTGGCG
CTGATTCGCGCGGATGAAGAGTTGATCTCCTCTCGTGGCTTTGTGTCTTCAGACGTCCGCACCAACACGATTAGCGTTCG
TGAGACGCAGCAGAAGCTGGAAGAGATTCGTCGCCTTGTCGGCACTTGGGATGTGCCTGTACGGCAGGTGTCCATCGAAG
CCCGAATTGTTCGCGCGCAGACCAATGTGGCTGAGAATCTCGGGGTTCGCTGGGGTGGTGCGGCTTACGATGTCAGTGGC
AATGATGTCATTAGTATCGGCGGATCGTTGGGTACCCTTCAGGAGGCGCGTGATGCCGCAGCCGGTGGTGGTGGTGGGAT
TTCGTTCCCCGGCGCCCTGGGAGTCGATCTGGGAGTTTCTGGCGACGGTGCCTCATCCTTTGCCATTGGCTGGGGTAGTG
ACGACTTCCTGATCGACCTTGAGCTTTCTGCTCTGGAAACAGACGGTCGCGCCGAGGTTGTCTCTCAGCCACGCGTGGTG
ACAGCAGATCGCCAGAGTGCGTCCATTAAGTCAGGTGAGGAGATTCCCTATCAGGAAGCGACCTCCAGTGGCGCGACTAA
CGTTGAGTTCAAGGAAGCGGTTCTTTCACTGGAGGTAACCCCGCAGATCACTCCGGATGACAAGATCATCATGGACCTGG
TGGTAAACCAGGATTCCCGGGGCGAGGTAACGGCTGGTATACCCTCTATCAACACCAACTCCGTGACCACGCAGGTCTTG
GTAGCCAACGGCGAAACTGTTGTATTGGGCGGTATCTTCCAGTCGGAAGTGGCTACACAAACCACCAAGACGCCGTTCCT
GGGGGATATTCCCTACCTCGGTCGCCTGTTCAAGCGCACGGAACACATCGATGAGCGCAGCGAATTGCTGATTTTCATCA
CACCCAAGATCATCAAGAACGACTTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(387-449)

AMIN domain (AMIN)

(56-137)

Bacterial type II and III secretion system protein (Secretin)

(544-699)

Secretin and TonB N terminus short domain (STN)

(313-359)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

47.546

100

0.482
  pilQ Acinetobacter baumannii strain A118

39.641

100

0.408
  pilQ Acinetobacter baumannii D1279779

39.641

100

0.408
  comQ Acinetobacter baylyi ADP1

40.17

100

0.403
  pilQ Legionella pneumophila strain ERS1305867

39.911

95.739

0.382
  pilQ Legionella pneumophila strain Lp02

39.763

95.739

0.381

Multiple sequence alignment