Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FIU96_RS03215 Genome accession   NZ_CP045367
Coordinates   699456..700520 (+) Length   354 a.a.
NCBI ID   WP_152437317.1    Uniprot ID   -
Organism   Marinobacter sp. THAF39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 694456..705520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU96_RS03200 (FIU96_03245) rpmE 694893..695114 (+) 222 WP_058090079.1 50S ribosomal protein L31 -
  FIU96_RS03205 (FIU96_03250) - 695331..696596 (+) 1266 WP_061332389.1 malic enzyme-like NAD(P)-binding protein -
  FIU96_RS03210 (FIU96_03255) - 696689..699217 (-) 2529 WP_152437316.1 penicillin-binding protein 1A -
  FIU96_RS03215 (FIU96_03260) pilM 699456..700520 (+) 1065 WP_152437317.1 pilus assembly protein PilM Machinery gene
  FIU96_RS03220 (FIU96_03265) - 700520..701083 (+) 564 WP_106693178.1 PilN domain-containing protein -
  FIU96_RS03225 (FIU96_03270) - 701083..701694 (+) 612 WP_072678328.1 type 4a pilus biogenesis protein PilO -
  FIU96_RS03230 (FIU96_03275) - 701697..702239 (+) 543 WP_061332384.1 pilus assembly protein PilP -
  FIU96_RS03235 (FIU96_03280) pilQ 702258..704372 (+) 2115 WP_152437318.1 type IV pilus secretin PilQ Machinery gene
  FIU96_RS03240 (FIU96_03285) aroK 704496..705080 (+) 585 WP_138438137.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38090.23 Da        Isoelectric Point: 4.1974

>NTDB_id=393671 FIU96_RS03215 WP_152437317.1 699456..700520(+) (pilM) [Marinobacter sp. THAF39]
MFGLLGKKSSAVLGVDVSSSSVKLLELSRHGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTGVKQVAV
AVSGSAVITKLIQMDGGLNEFEMEDQITLEADQYIPYPLDEVAIDFEVQGPSESNPDQVDVLLAACRKENVDIREDALEI
AGLTTKIVDVEAYALERAYKLIEPQLESQGEELVVAIVDIGATMTTLSVLAEGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYEPEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FADMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=393671 FIU96_RS03215 WP_152437317.1 699456..700520(+) (pilM) [Marinobacter sp. THAF39]
GTGTTCGGATTGCTAGGAAAAAAATCCAGTGCAGTGCTGGGCGTGGATGTCAGCTCCAGCTCGGTCAAACTTCTGGAACT
GTCAAGGCATGGCGACCGTTACAAGGTTGAAAGCTATGCGGTTGAGCCGTTACCGGCCAACGCAGTTGTCGAGAAAAACA
TCACTGATGTAGAAGCGGTGGGTGAGGTGCTCAAGCGAGTGGCCTCTAAATCCCGTACCGGCGTTAAGCAGGTTGCCGTG
GCTGTGTCAGGTTCTGCAGTTATCACCAAGCTCATCCAGATGGATGGCGGCCTCAACGAATTCGAGATGGAAGACCAGAT
CACCCTCGAGGCCGATCAGTACATCCCGTATCCGCTGGATGAAGTAGCTATCGATTTTGAGGTGCAGGGTCCGTCTGAGT
CGAATCCGGACCAGGTCGACGTGCTTCTGGCTGCGTGCAGAAAAGAGAACGTGGATATCCGTGAGGACGCTCTCGAGATT
GCCGGCCTGACCACCAAGATTGTCGATGTCGAAGCTTATGCACTGGAAAGGGCGTACAAACTGATCGAACCCCAATTGGA
GTCCCAGGGCGAAGAGCTGGTGGTTGCCATCGTCGATATCGGCGCAACCATGACCACGCTGAGTGTGCTGGCTGAGGGCA
AGACAGTTTATACCCGAGAGCAGATTTTCGGGGGCAAGCAGCTTACTGAGGAAATTCAGCGCCGCTACGGCTTATCACTT
GAAGAAGCCGGCCTGGCCAAGAAGCAGGGCGGGCTCCCGGACGACTATGAACCGGAGGTGCTTACCCCCTTCCGTGAAGC
GGTTGTGCAGCAGGTTGCACGCGCTCTCCAGTTTTTCTTTGGTGCCAGCCAGTACAACGCCGTTGATTACGTCGTTCTTG
CGGGCGGTACGGCATCCATTCAGGGCCTGACCGAGATGGTGGAGGAGAAGACCGGCACGCCAACTCTGGTTGCGAATCCA
TTCGCCGACATGGCCGTGGGTTCCAGAGTGAATGCATCAGCCCTGAGCAATGATGCTCCATCGTTGATGATTGCCTGCGG
CCTGGCAATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.672

100

0.537
  comM Acinetobacter nosocomialis M2

53.672

100

0.537
  comM Acinetobacter baylyi ADP1

51.412

100

0.514
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.455

Multiple sequence alignment