Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FIV02_RS04035 Genome accession   NZ_CP045349
Coordinates   880893..881309 (+) Length   138 a.a.
NCBI ID   WP_152487868.1    Uniprot ID   -
Organism   Pseudomonas sp. THAF187a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 880893..893755 880893..881309 within 0


Gene organization within MGE regions


Location: 880893..893755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV02_RS04035 (FIV02_03995) pilA 880893..881309 (+) 417 WP_152487868.1 pilin Machinery gene
  FIV02_RS04040 (FIV02_04000) - 881326..883236 (+) 1911 WP_152487869.1 hypothetical protein -
  FIV02_RS04045 (FIV02_04005) - 883236..884090 (+) 855 WP_152487870.1 glycosyltransferase family 2 protein -
  FIV02_RS04050 (FIV02_04010) - 884212..885114 (+) 903 WP_152487871.1 glycosyltransferase -
  FIV02_RS04055 (FIV02_04015) - 885171..886388 (+) 1218 WP_152487872.1 lipopolysaccharide biosynthesis protein -
  FIV02_RS25165 - 886768..887424 (+) 657 WP_371923266.1 class I SAM-dependent methyltransferase -
  FIV02_RS04065 (FIV02_04025) - 887684..888421 (+) 738 WP_172979125.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  FIV02_RS04070 (FIV02_04030) - 888418..889281 (+) 864 WP_152487875.1 glycosyltransferase family 2 protein -
  FIV02_RS04075 (FIV02_04035) - 889278..890393 (-) 1116 WP_152487876.1 glycosyltransferase family 4 protein -
  FIV02_RS04080 (FIV02_04040) - 890444..892372 (-) 1929 WP_152487877.1 acyltransferase family protein -
  FIV02_RS04085 (FIV02_04045) - 892445..893755 (-) 1311 WP_152487878.1 nucleotide sugar dehydrogenase -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14446.50 Da        Isoelectric Point: 5.6081

>NTDB_id=393529 FIV02_RS04035 WP_152487868.1 880893..881309(+) (pilA) [Pseudomonas sp. THAF187a]
MQYARGFTLIELMIVVAIIGILAAIALPAYQNYVAKSQLAAGLSDIAGGRPSYEALVVTENVSSFNLSDIGLVSETSRCT
VSMNYDSADGTGDITCTVKGSPIVNGKTIQLVRSSSGAWKCQVDASISAIYHPQGCAH

Nucleotide


Download         Length: 417 bp        

>NTDB_id=393529 FIV02_RS04035 WP_152487868.1 880893..881309(+) (pilA) [Pseudomonas sp. THAF187a]
ATGCAATACGCACGAGGCTTTACGCTGATCGAGCTGATGATTGTTGTGGCAATAATTGGTATTTTGGCGGCCATTGCCTT
GCCGGCCTATCAAAATTATGTAGCCAAGTCCCAGTTGGCTGCTGGTCTATCGGATATTGCCGGTGGTCGTCCGTCTTATG
AGGCTTTAGTGGTAACCGAGAATGTCAGCAGCTTTAACCTGAGCGATATTGGCTTAGTGAGTGAGACCTCACGCTGTACC
GTCAGTATGAATTATGACAGCGCCGACGGTACTGGCGACATCACTTGTACGGTTAAAGGCAGCCCGATAGTCAATGGTAA
AACTATTCAGTTAGTACGCTCCAGTTCCGGCGCTTGGAAATGCCAAGTGGATGCCTCTATCTCCGCTATTTATCATCCCC
AAGGTTGCGCCCATTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.201

100

0.486
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.39

100

0.464
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.985

99.275

0.457
  pilA Pseudomonas aeruginosa PAK

39.869

100

0.442
  pilA Vibrio cholerae C6706

37.762

100

0.391
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.762

100

0.391
  pilA Vibrio cholerae strain A1552

37.762

100

0.391
  pilA2 Legionella pneumophila str. Paris

37.313

97.101

0.362

Multiple sequence alignment