Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIV04_RS12225 Genome accession   NZ_CP045338
Coordinates   2755331..2757016 (+) Length   561 a.a.
NCBI ID   WP_152469277.1    Uniprot ID   -
Organism   Vibrio sp. THAF190c     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2750331..2762016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV04_RS12200 (FIV04_12255) pdhR 2751842..2752609 (-) 768 WP_032550147.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FIV04_RS12205 (FIV04_12260) ampD 2753058..2753606 (-) 549 WP_152469275.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FIV04_RS12215 (FIV04_12265) nadC 2753755..2754645 (+) 891 WP_032550149.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FIV04_RS12220 (FIV04_12270) pilA 2754899..2755330 (+) 432 WP_152469276.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FIV04_RS12225 (FIV04_12275) pilB 2755331..2757016 (+) 1686 WP_152469277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIV04_RS12230 (FIV04_12280) pilC 2757063..2758292 (+) 1230 WP_172974246.1 type II secretion system F family protein Machinery gene
  FIV04_RS12235 (FIV04_12285) pilD 2758352..2759221 (+) 870 WP_152469278.1 A24 family peptidase Machinery gene
  FIV04_RS12240 (FIV04_12290) coaE 2759225..2759833 (+) 609 WP_152469279.1 dephospho-CoA kinase -
  FIV04_RS12245 (FIV04_12295) zapD 2759866..2760606 (+) 741 WP_032550155.1 cell division protein ZapD -
  FIV04_RS12250 (FIV04_12300) yacG 2760694..2760891 (+) 198 WP_032550156.1 DNA gyrase inhibitor YacG -
  FIV04_RS12260 (FIV04_12305) rplS 2761292..2761645 (-) 354 WP_032549758.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62390.87 Da        Isoelectric Point: 5.3509

>NTDB_id=393493 FIV04_RS12225 WP_152469277.1 2755331..2757016(+) (pilB) [Vibrio sp. THAF190c]
MLTNLPTVLRQANHLSLTQEQAVVEHLQASGGSTPEALIAHGLFSADDLAEYTAHIFGLPLISLADYDYELLCEQLGLRE
LITQHQVIPIAKSASTLTLASADPTDLQAEDNFRFATGLQIELVVANYHTLEGAIRRLYGKVITNGDSNRKEITQDELAH
LVEISDDEVSTVEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEYYRVRLRCDGILIETQQPAAHLSRRLSARLKI
LSKLDIAERRLPQDGRIKLRLNQEIAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDILGYSEQQKTLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSERNISTAEDPVEINLCGVNQVQVQPKIGFGFAEALRSFLRQDPDIVMVGEIRDIET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLTHMGIEHFNLASSLSLIIAQRLARRLCSHCKIEDDDQTIYHHHNIDANHR
FFKANPNGCNECNQGYSGRVGIYEVMPFTDTLKASLISHPNAIEVEALAKQEGMQTLRDSGLEKLLSGVTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=393493 FIV04_RS12225 WP_152469277.1 2755331..2757016(+) (pilB) [Vibrio sp. THAF190c]
GTGTTAACCAACCTCCCCACTGTCCTTCGTCAGGCTAACCACCTTAGCCTGACTCAAGAACAAGCCGTAGTGGAGCACTT
GCAAGCTTCTGGTGGCTCAACGCCAGAAGCTTTAATCGCGCATGGCTTGTTTAGCGCCGATGACCTTGCCGAATACACAG
CACATATCTTTGGTTTACCTTTAATTTCGCTAGCGGATTACGATTACGAGCTGTTATGCGAACAGCTCGGGCTGCGCGAA
CTCATCACTCAGCACCAAGTCATACCGATCGCCAAGTCAGCATCTACGCTGACCTTAGCCTCTGCCGACCCAACTGACCT
ACAAGCTGAAGATAACTTTCGTTTTGCCACTGGACTTCAGATTGAACTGGTGGTTGCAAACTATCACACACTTGAAGGTG
CCATTCGGCGGCTTTACGGCAAAGTAATTACCAATGGTGATAGCAATCGTAAAGAGATAACCCAAGATGAACTCGCTCAC
TTAGTGGAAATTTCTGACGATGAGGTCAGCACTGTCGAAGACCTTAGCCAAGATGACTCGCCCGTCAGTCGCTTTATCAA
TCAAATCCTAGTCGATGCGGTGCGTAAAGGGGCATCTGATATCCATTTCGAGCCTTATGAAGAGTACTACCGAGTCCGCC
TACGGTGCGATGGCATCTTAATCGAAACCCAACAACCGGCAGCGCACCTAAGTCGACGACTGTCTGCTCGCCTGAAGATC
CTATCCAAGCTTGATATCGCGGAGCGACGTTTACCTCAAGATGGTCGGATTAAGTTAAGGCTCAATCAAGAGATCGCTAT
CGATATGCGCGTCTCTACTCTGCCAACACTTTGGGGTGAAAAAATTGTGCTTCGTCTACTCGATAGCAGCGCTGCCAATC
TTGATATCGACATCCTAGGCTATAGTGAGCAACAAAAGACACTCTACCTTAATGCTCTGAAACGCCCACAAGGCATGATT
CTTATGACTGGGCCTACCGGTAGTGGCAAAACGGTATCGCTTTATACTGGACTTCGAATCCTCAATACCTCAGAAAGAAA
CATCTCTACCGCTGAAGACCCAGTTGAGATTAACCTTTGCGGCGTCAATCAAGTGCAAGTACAACCAAAGATAGGCTTTG
GATTTGCTGAAGCATTGCGATCTTTTCTGCGCCAAGATCCGGATATCGTGATGGTTGGTGAAATACGCGATATTGAAACA
GCCGAGATTGCGATTAAGGCTTCCCAAACCGGTCACTTAGTGCTCTCAACGCTACACACCAATTCAGCAGCAGAGACGGT
TACACGCCTTACTCACATGGGGATTGAGCATTTTAACTTGGCTTCATCATTGAGCCTCATCATTGCTCAACGTCTCGCTC
GACGACTATGCTCACATTGCAAAATTGAAGACGATGACCAAACCATTTATCACCATCACAACATTGATGCTAACCATAGG
TTCTTTAAAGCAAACCCGAACGGCTGTAACGAGTGCAATCAAGGGTATTCAGGCCGTGTGGGTATCTATGAAGTGATGCC
GTTCACTGACACGCTGAAAGCAAGCCTCATATCTCACCCAAATGCGATTGAGGTAGAAGCCCTAGCTAAACAAGAAGGCA
TGCAAACACTCCGAGATTCGGGCTTAGAGAAATTGTTGTCCGGGGTCACTAGCTATCAAGAATTGCAACGCGTCCTCTAT
CTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

71.886

100

0.72
  pilB Vibrio parahaemolyticus RIMD 2210633

69.697

100

0.697
  pilB Vibrio campbellii strain DS40M4

69.697

100

0.697
  pilB Acinetobacter baumannii D1279779

49.46

99.109

0.49
  pilB Legionella pneumophila strain ERS1305867

49.716

94.296

0.469
  pilB Acinetobacter baylyi ADP1

49.62

93.761

0.465
  pilF Neisseria gonorrhoeae MS11

42.908

100

0.431
  pilF Thermus thermophilus HB27

38.061

99.287

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.989

95.722

0.364

Multiple sequence alignment