Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG/mmsA   Type   Machinery gene
Locus tag   HPC60_RS12920 Genome accession   NZ_CP053671
Coordinates   2537305..2539305 (-) Length   666 a.a.
NCBI ID   WP_023190222.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain G121     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2532305..2544305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPC60_RS12895 (HPC60_07645) - 2532778..2533392 (+) 615 WP_023190218.1 TetR/AcrR family transcriptional regulator -
  HPC60_RS12900 (HPC60_07650) - 2533471..2534199 (-) 729 WP_025017062.1 pseudouridine synthase -
  HPC60_RS12905 (HPC60_07655) - 2534295..2534930 (-) 636 WP_003132036.1 hypothetical protein -
  HPC60_RS12910 (HPC60_07660) mnmE 2535141..2536508 (+) 1368 WP_023190220.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  HPC60_RS12915 (HPC60_07665) - 2536559..2537305 (-) 747 WP_023190221.1 hypothetical protein -
  HPC60_RS12920 (HPC60_07670) recG/mmsA 2537305..2539305 (-) 2001 WP_023190222.1 ATP-dependent DNA helicase RecG Machinery gene
  HPC60_RS12925 (HPC60_07675) - 2539443..2539787 (-) 345 WP_012898719.1 zinc ribbon domain-containing protein YjdM -
  HPC60_RS12930 (HPC60_07680) gap 2539857..2540867 (-) 1011 WP_003131244.1 type I glyceraldehyde-3-phosphate dehydrogenase -
  HPC60_RS12935 (HPC60_07685) - 2541148..2541669 (-) 522 WP_003131246.1 DUF536 domain-containing protein -

Sequence

Protein


Download         Length: 666 a.a.        Molecular weight: 74888.54 Da        Isoelectric Point: 5.6980

>NTDB_id=393404 HPC60_RS12920 WP_023190222.1 2537305..2539305(-) (recG/mmsA) [Lactococcus lactis subsp. lactis strain G121]
MKLTDSVQFLKGVGPKSVENFHKLGIFTVQDLLLYFPFRYEDFASRSVFELVDGEKATIIGTVVTPANVQYYGFKRNRLS
FKIKEGEAVVAVSFFNQPYLADKVEVGKEIAVYGKWEQAKQQLMGMKVVAQVDDGLEPVYHLTAGMKQSQLVKVIHQVFE
SDLLSELPENLPEFLIKKYRLMNRQEAVLAMHFPEDTEAHKQALRRVKFEELFMFQLKLQALKNKEKSGRAGLEVVFEQG
EIDQKISELPFELTDAQLSALSEILTDMKSPYHMNRLLQGDVGSGKTVVASLAMYAACLANFQAAIMVPTEILARQHFAN
LKALFPELQISLLVSGLKVAERREILADLVSGHTHMVVGTHALIQEGVDFYNLGLVITDEQHRFGVNQRKILREKGQNPD
VLMMTATPIPRTLAITAFGDMDVSIIDELPKGRQPITTRWVKHEQFSEVLSWIKAEVAKDSQVYFISPLIEESETLDLKN
AEALFAELNEYFGLFAKIGLLHGKMKNDEKDQIMQEFKAKKLDILVSTTVIEVGVDVPNATIMVIMDADRFGLSQLHQLR
GRVGRGTKKSYAILVANPKSDSGKQRMKIMTETQNGFILAEEDLKMRGSGEIFGLRQSGLPEFIAADLVNDYNILEVARK
EAVEIFKNADEFPDLIAQVNVDGGFD

Nucleotide


Download         Length: 2001 bp        

>NTDB_id=393404 HPC60_RS12920 WP_023190222.1 2537305..2539305(-) (recG/mmsA) [Lactococcus lactis subsp. lactis strain G121]
ATGAAATTAACAGATTCAGTGCAATTTTTAAAAGGTGTGGGACCGAAATCTGTGGAAAATTTCCATAAGTTAGGAATTTT
CACGGTTCAAGATTTATTGCTTTATTTCCCTTTTCGTTATGAAGATTTCGCAAGTCGTTCGGTTTTTGAACTTGTTGACG
GAGAAAAAGCAACAATTATTGGAACGGTAGTGACACCAGCTAATGTCCAGTATTACGGATTTAAACGTAATCGACTTTCA
TTTAAAATTAAAGAAGGTGAGGCCGTGGTTGCTGTCAGCTTTTTTAATCAACCTTATTTGGCTGACAAAGTGGAAGTTGG
TAAGGAAATTGCAGTTTACGGAAAATGGGAGCAGGCAAAGCAACAACTGATGGGGATGAAAGTTGTGGCTCAGGTGGACG
ATGGCTTAGAACCTGTTTATCATTTGACAGCTGGTATGAAACAAAGTCAATTGGTCAAAGTAATTCATCAGGTTTTTGAA
TCAGACCTTCTATCAGAATTACCTGAGAACTTACCAGAATTTTTGATTAAAAAATATCGTCTGATGAACCGGCAAGAAGC
CGTTTTGGCTATGCATTTTCCTGAAGATACGGAAGCTCATAAGCAGGCTTTACGTCGTGTAAAATTTGAAGAATTATTCA
TGTTTCAGTTGAAATTACAGGCGTTAAAAAATAAAGAAAAGTCGGGCCGAGCAGGATTAGAAGTTGTTTTTGAGCAAGGA
GAAATTGATCAGAAGATTTCTGAATTACCTTTTGAACTGACAGATGCTCAATTAAGCGCTTTGTCAGAGATTCTGACGGA
TATGAAATCTCCTTATCACATGAATCGACTTTTGCAAGGTGATGTGGGTTCTGGTAAAACAGTTGTTGCTTCCCTTGCCA
TGTACGCGGCTTGTTTGGCGAATTTTCAAGCAGCAATTATGGTACCAACGGAAATCCTTGCCCGTCAGCATTTTGCAAAC
TTGAAAGCTCTTTTTCCAGAATTGCAAATTTCACTTTTGGTTTCGGGTTTGAAAGTGGCAGAGCGCCGCGAGATTTTGGC
TGATTTAGTTAGTGGTCATACACATATGGTAGTTGGAACTCATGCACTAATTCAAGAAGGGGTCGATTTTTATAATTTGG
GTCTTGTGATTACAGATGAACAACACCGTTTCGGAGTTAATCAGCGAAAAATTTTACGAGAAAAAGGGCAAAATCCAGAT
GTTCTAATGATGACAGCTACACCCATTCCAAGAACGCTTGCGATTACGGCTTTTGGGGATATGGATGTTTCAATTATTGA
TGAACTACCCAAGGGACGTCAACCCATTACTACAAGGTGGGTCAAACATGAACAATTTTCCGAAGTTTTAAGTTGGATAA
AAGCAGAGGTTGCTAAAGATTCACAGGTTTATTTTATTTCACCTTTAATTGAGGAAAGTGAAACGTTGGACCTGAAAAAC
GCAGAAGCTCTTTTTGCGGAATTAAATGAATACTTTGGATTATTTGCCAAAATTGGTCTTTTACATGGAAAAATGAAAAA
TGATGAAAAAGACCAAATCATGCAAGAATTTAAGGCTAAAAAATTAGATATTTTGGTTTCAACAACAGTGATTGAAGTGG
GAGTTGACGTTCCTAATGCTACCATTATGGTGATTATGGATGCTGACCGCTTTGGACTTTCGCAGCTTCACCAATTGCGT
GGTCGTGTTGGTCGGGGAACAAAAAAATCTTATGCCATTTTAGTAGCAAATCCTAAGTCCGATTCAGGAAAACAACGGAT
GAAAATTATGACAGAAACACAAAATGGTTTTATTTTAGCCGAAGAAGATTTGAAAATGCGTGGTTCTGGAGAGATTTTTG
GACTTCGTCAGTCAGGACTTCCAGAATTTATTGCGGCTGATTTAGTCAATGATTATAATATTTTAGAAGTGGCTAGAAAA
GAAGCAGTTGAAATTTTTAAAAATGCAGATGAGTTTCCTGATTTGATTGCTCAAGTTAATGTTGACGGGGGCTTTGATTG
A

Domains

Predicted by InterProScan.

RecG wedge domain (RecG_wedge)

(7-157)

DEAD/DEAH box helicase (DEAD)

(254-411)

Helicase conserved C-terminal domain (Helicase_C)

(456-566)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(593-651)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG/mmsA Streptococcus pneumoniae R6

66.047

96.847

0.64
  recG/mmsA Streptococcus pneumoniae R36A

66.047

96.847

0.64
  recG Bacillus subtilis subsp. subtilis str. 168

50.698

96.847

0.491
  recG Neisseria meningitidis strain C311

40.332

99.399

0.401

Multiple sequence alignment