Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GB930_RS19810 Genome accession   NZ_CP045311
Coordinates   4347855..4349570 (-) Length   571 a.a.
NCBI ID   WP_185450531.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain Aer_OnIF1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4342855..4354570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GB930_RS19780 (GB930_19820) mutT 4343530..4343943 (+) 414 WP_242762034.1 8-oxo-dGTP diphosphatase MutT -
  GB930_RS19785 (GB930_19825) yacG 4344005..4344199 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  GB930_RS19790 (GB930_19830) zapD 4344209..4344931 (-) 723 WP_025202564.1 cell division protein ZapD -
  GB930_RS19795 (GB930_19835) coaE 4344969..4345583 (-) 615 WP_242762039.1 dephospho-CoA kinase -
  GB930_RS19800 (GB930_19840) pilD 4345603..4346475 (-) 873 WP_042007258.1 A24 family peptidase Machinery gene
  GB930_RS19805 (GB930_19845) pilC 4346498..4347739 (-) 1242 WP_010635960.1 type II secretion system F family protein Machinery gene
  GB930_RS19810 (GB930_19850) pilB 4347855..4349570 (-) 1716 WP_185450531.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GB930_RS19815 (GB930_19855) - 4349561..4350001 (-) 441 WP_010635961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GB930_RS19820 (GB930_19860) nadC 4350323..4351177 (-) 855 WP_242762041.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GB930_RS19825 (GB930_19865) - 4351182..4351652 (-) 471 WP_095591545.1 retropepsin-like aspartic protease -
  GB930_RS19830 (GB930_19870) ampD 4351776..4352348 (+) 573 WP_242762042.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GB930_RS19835 (GB930_19875) pdhR 4352624..4353388 (+) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62871.40 Da        Isoelectric Point: 5.4363

>NTDB_id=393398 GB930_RS19810 WP_185450531.1 4347855..4349570(-) (pilB) [Aeromonas dhakensis strain Aer_OnIF1]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMESDQDALGMEEIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=393398 GB930_RS19810 WP_185450531.1 4347855..4349570(-) (pilB) [Aeromonas dhakensis strain Aer_OnIF1]
CTGCCCATAATGACATCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAAATCCTCGACAGCA
AGGCGCTGGCCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCCGACCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTGCACACCGAGGCCTTGCTGGTCG
AGGAGAATAAGCTGACCGCCGCCATCAGCAAGCTTATGGAGAGCGATCAGGACGCCCTTGGCATGGAGGAGATCGACGAG
TCCGAGATTTCCGAGCTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCAATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAAA
CCAAGTACCGTATTCGCTTTCGGATAGACGGAATCCTGCATGAGGTAGCAACGCCGCCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGACTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAGAT
GTCGCGCAACAAGTCGATGGATATGCGGGTCAATACCCTACCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCG
ACTCTTCCGCTGCGCGCCTCAATATCGAACAGCTCGGCTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCGCTCTCT
AAGCCTCAGGGCATGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATCCT
CAACACCACAGAGGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCTGGGGTCAATCAGGTACAGGTCA
ACCCCAAGGCCGGCCTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCAGATGTGGTGATGGTCGGGGAA
ATACGGGATCTTGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCTACATTGCACACCAA
CTCGGCGGCCGAGACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCA
TGGCTCAGCGACTGGCACGCAAGCTGTGCGACCACTGCAAGGCTCCCGAAGTGGTGCCGGAAGCTGAACTGCTGGAGTTG
GGGTTCACCCAACAGCAGCTGGCCGCCGGCTTGCGGCTGTTCAAGCCGGTCGGCTGCAAGGAGTGTTCTGGCGGCTACAA
GGGACGGGTCGGCATCTACGAAATCCTGTTGATGTCCGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGC
AGATTGCGGCTATCGCCCAGAAGGAGGGCATGCGCACCTTGCGGACCTCCGGTCTTGAGAAAGCCCGACTGGGGGTGACC
AGCCTGGCCGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

54.783

100

0.552
  pilB Legionella pneumophila strain ERS1305867

54.594

99.124

0.541
  pilB Vibrio cholerae strain A1552

54.159

98.949

0.536
  pilB Vibrio campbellii strain DS40M4

51.767

99.124

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.424

0.503
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

96.848

0.375
  pilF Thermus thermophilus HB27

40.53

92.469

0.375

Multiple sequence alignment