Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GDV60_RS23045 Genome accession   NZ_CP045254
Coordinates   5218342..5219562 (+) Length   406 a.a.
NCBI ID   WP_159908853.1    Uniprot ID   A0AAJ4BNA5
Organism   Pseudomonas sp. DTU12.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5213342..5224562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GDV60_RS23020 (GDV60_23650) nadC 5214775..5215623 (+) 849 WP_159908847.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GDV60_RS23025 (GDV60_23655) - 5215690..5215995 (-) 306 WP_159908849.1 DUF6388 family protein -
  GDV60_RS23035 (GDV60_23665) - 5216244..5217650 (-) 1407 WP_159908851.1 O-antigen ligase -
  GDV60_RS23040 (GDV60_23670) - 5217728..5218138 (-) 411 WP_029291989.1 pilin -
  GDV60_RS23045 (GDV60_23675) pilC 5218342..5219562 (+) 1221 WP_159908853.1 type II secretion system F family protein Machinery gene
  GDV60_RS23050 (GDV60_23680) pilD 5219565..5220437 (+) 873 WP_159908855.1 A24 family peptidase Machinery gene
  GDV60_RS23055 (GDV60_23685) coaE 5220434..5221057 (+) 624 WP_159908857.1 dephospho-CoA kinase -
  GDV60_RS23060 (GDV60_23690) yacG 5221054..5221257 (+) 204 WP_092238375.1 DNA gyrase inhibitor YacG -
  GDV60_RS23065 (GDV60_23695) - 5221254..5221469 (-) 216 WP_029292000.1 hypothetical protein -
  GDV60_RS23070 (GDV60_23700) - 5221533..5222222 (-) 690 WP_029292001.1 energy-coupling factor ABC transporter permease -
  GDV60_RS23075 (GDV60_23705) - 5222219..5222710 (-) 492 WP_169390310.1 FAD/FMN-containing dehydrogenase -
  GDV60_RS23080 (GDV60_23710) - 5222789..5223418 (+) 630 WP_029292005.1 DUF1780 domain-containing protein -
  GDV60_RS23085 (GDV60_23715) - 5223458..5223631 (+) 174 WP_104910930.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 43967.71 Da        Isoelectric Point: 10.1358

>NTDB_id=392961 GDV60_RS23045 WP_159908853.1 5218342..5219562(+) (pilC) [Pseudomonas sp. DTU12.1]
MNDVTVSPSATYAWEGTLRNGRKVSGETRGHSPAVIKAQLRRQGITPVRVHVRSRLLCGLGNPITPADIALFTRQLATLL
KAGIPLLQAFDMIGEGFDSRAMRTMVQGLKQEIAAGNSLTAALSKQPQCFNPLYCSLIAAGEQAGTLETLLERVATHLEK
SQRLKARIKKAMTYPLVVVLVAMLVSSILLIHVVPQFESLFAGIDTPLPRFTLLVIGLSEFMQGAWWMLLLTAIASAWGI
RRAYRRHNGLRLWLDTGLLKLPLAGTLLKKTAVARYARTLATTFAAGVPLAQALDSVAAAAGNERFTQAITRMRQDVAIG
MQLNRTMTASGLFPGMAIQMTAIGEESGALDSMLEKVADHYETDVDNLVDNLSSLMEPLIMVVLGSIVGALVIAMYLPIF
QLGTAF

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=392961 GDV60_RS23045 WP_159908853.1 5218342..5219562(+) (pilC) [Pseudomonas sp. DTU12.1]
ATGAATGATGTCACTGTGAGCCCCTCCGCCACCTATGCTTGGGAAGGCACGTTGCGCAATGGCCGCAAGGTGTCCGGCGA
AACCCGTGGCCATAGCCCGGCCGTGATCAAGGCACAACTGCGCCGGCAAGGCATAACCCCGGTTCGGGTACACGTGCGCT
CCAGACTCCTTTGCGGCCTGGGCAACCCAATCACCCCAGCCGATATTGCGCTGTTCACCCGCCAACTGGCGACCCTGCTG
AAGGCGGGAATTCCCTTGCTACAAGCCTTCGACATGATTGGCGAAGGCTTTGACAGCCGGGCCATGCGCACAATGGTCCA
AGGGCTGAAGCAGGAGATTGCCGCCGGCAACAGCCTGACCGCTGCGCTATCGAAACAGCCGCAGTGCTTCAACCCCCTGT
ATTGCAGCCTGATCGCGGCCGGCGAACAGGCCGGCACCCTGGAGACCTTGCTGGAGCGGGTAGCCACCCACCTGGAGAAG
AGCCAGCGCCTCAAGGCCCGGATCAAGAAGGCCATGACCTATCCGCTGGTGGTCGTGCTCGTGGCGATGCTGGTCAGCAG
CATCTTGCTGATCCACGTCGTGCCCCAATTCGAAAGCCTGTTCGCCGGCATCGACACACCGCTGCCACGCTTTACCCTGC
TGGTCATCGGGCTGTCCGAATTCATGCAAGGCGCATGGTGGATGCTATTGCTGACCGCAATCGCAAGTGCCTGGGGAATA
CGCCGCGCCTACCGCCGACACAACGGTTTGCGCCTGTGGCTGGACACGGGTTTGTTGAAACTGCCGCTGGCAGGCACACT
GCTGAAAAAAACCGCAGTCGCCCGCTACGCTCGCACACTCGCCACCACCTTTGCCGCTGGCGTACCGCTGGCACAGGCGC
TGGACTCAGTGGCCGCAGCCGCCGGCAACGAGCGGTTCACACAGGCAATCACGCGTATGCGCCAGGACGTGGCAATAGGA
ATGCAATTGAATCGAACCATGACCGCCAGCGGCCTGTTCCCTGGCATGGCGATCCAGATGACAGCCATCGGCGAAGAATC
AGGAGCGCTGGACAGCATGCTGGAGAAGGTCGCCGATCATTATGAAACGGACGTCGACAACCTGGTGGACAACCTCAGCA
GCCTGATGGAGCCGCTGATCATGGTGGTGCTCGGCAGCATTGTCGGGGCACTGGTAATCGCCATGTACCTGCCGATCTTC
CAGCTTGGCACGGCGTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(277-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.828

97.537

0.564
  pilC Acinetobacter baylyi ADP1

51.768

97.537

0.505
  pilC Acinetobacter baumannii D1279779

50.758

97.537

0.495
  pilC Legionella pneumophila strain ERS1305867

46.814

100

0.47
  pilC Vibrio cholerae strain A1552

40.704

98.03

0.399
  pilG Neisseria gonorrhoeae MS11

39.34

97.044

0.382
  pilC Vibrio campbellii strain DS40M4

38.5

98.522

0.379
  pilG Neisseria meningitidis 44/76-A

39.108

93.842

0.367

Multiple sequence alignment