Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GDV60_RS02850 Genome accession   NZ_CP045254
Coordinates   649102..650433 (+) Length   443 a.a.
NCBI ID   WP_092232908.1    Uniprot ID   A0AAW5A4N3
Organism   Pseudomonas sp. DTU12.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644102..655433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GDV60_RS02830 (GDV60_02915) pilM 646699..647352 (+) 654 WP_133714080.1 type IV pilus biogenesis protein PilM -
  GDV60_RS02835 (GDV60_02920) - 647352..647903 (+) 552 WP_029296063.1 PilN domain-containing protein -
  GDV60_RS02840 (GDV60_02925) - 647900..648520 (+) 621 WP_104911417.1 type 4a pilus biogenesis protein PilO -
  GDV60_RS02845 (GDV60_02930) - 648517..649032 (+) 516 WP_159906210.1 pilus assembly protein PilP -
  GDV60_RS02850 (GDV60_02935) pilQ 649102..650433 (+) 1332 WP_092232908.1 type IV pilus secretin PilQ Machinery gene
  GDV60_RS02855 (GDV60_02940) aroK 650438..650956 (+) 519 WP_029296072.1 shikimate kinase AroK -
  GDV60_RS02860 (GDV60_02945) aroB 651104..652204 (+) 1101 WP_092232911.1 3-dehydroquinate synthase -
  GDV60_RS02865 (GDV60_02950) - 652215..653789 (+) 1575 WP_104911419.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 48006.91 Da        Isoelectric Point: 4.8365

>NTDB_id=392949 GDV60_RS02850 WP_092232908.1 649102..650433(+) (pilQ) [Pseudomonas sp. DTU12.1]
MTEAVPKRIDLIQLPPPGASPLGAGRGAYSGDRLSLNFQDIEVRAVLQQIADVAGLNLVAGDDVQGSITLRLKDVPWDQA
LDLVLQAKGLDKRVTANVLLVAPAEELAARELLALESRKLMTELAPLRRELLQVNYAKAAELAKLFQSVSGPEGVADERG
SVTVDDRTNNIIAYQTGERLEELRRIVNQLDIPVRQVMIEARIVEANVDYDKSVGVRWGGLLRNKGHWSGGGVGKSPSGE
GEPPDSSPFVDLGVVNASSGLGIAFITDNVLLDLELTAMEKGGNGEVISQPKVVTSNKETARILKGTEIPYQEATAEGAT
SVSFKEASLSLEVTPQITPDDQVIMEVRVTKDEPDYLNKLNDVPPIKKNEVTAKVLVRDGETIVLGGVFSKNQSKVVDKV
PFLGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=392949 GDV60_RS02850 WP_092232908.1 649102..650433(+) (pilQ) [Pseudomonas sp. DTU12.1]
ATGACTGAGGCTGTGCCCAAGCGGATTGACCTGATTCAGTTGCCACCCCCTGGTGCTTCGCCTTTGGGCGCCGGACGAGG
GGCCTATAGCGGTGACAGGCTCTCTCTCAACTTCCAGGACATCGAGGTCCGCGCAGTCCTGCAGCAGATTGCCGACGTGG
CTGGCCTGAACCTGGTGGCCGGTGATGATGTGCAGGGCTCGATCACCCTGCGGCTCAAGGACGTGCCCTGGGATCAGGCC
CTGGACCTGGTGCTGCAAGCCAAGGGGCTCGACAAGCGGGTGACGGCCAATGTGCTGCTGGTGGCGCCGGCTGAGGAACT
GGCCGCCCGTGAGCTGCTGGCCTTGGAGTCGCGCAAGCTGATGACCGAGCTGGCGCCCTTGCGGCGGGAGCTGCTGCAAG
TCAATTACGCCAAGGCGGCCGAGTTGGCCAAGCTGTTCCAGTCGGTGAGCGGGCCTGAGGGTGTGGCCGATGAGCGTGGT
TCGGTGACGGTAGATGACCGTACCAACAACATCATTGCCTACCAGACGGGGGAGCGCCTGGAGGAGCTGCGGCGCATCGT
CAATCAATTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATAGTCGAGGCCAATGTCGACTATGACAAAAGCG
TGGGCGTGCGCTGGGGTGGGTTGTTGCGCAATAAAGGTCATTGGAGCGGGGGTGGGGTTGGTAAAAGCCCGTCCGGCGAG
GGCGAGCCGCCCGATAGCTCGCCGTTCGTTGACCTGGGCGTGGTCAACGCAAGCTCTGGTCTGGGCATCGCGTTTATCAC
CGATAACGTGCTGCTGGACCTGGAACTCACAGCCATGGAAAAAGGCGGCAATGGCGAGGTGATCTCCCAGCCCAAGGTGG
TCACTTCCAACAAGGAGACCGCGCGGATTCTCAAGGGCACCGAGATCCCCTACCAGGAAGCCACTGCCGAGGGTGCGACG
TCGGTGTCGTTCAAGGAGGCCTCGCTTTCTTTGGAGGTGACGCCGCAAATCACCCCGGATGACCAGGTCATCATGGAGGT
CCGGGTGACCAAGGATGAGCCGGACTACCTGAACAAACTCAACGATGTGCCGCCGATCAAGAAGAACGAGGTCACGGCCA
AGGTTCTGGTCAGGGACGGCGAGACCATCGTCCTGGGCGGGGTTTTCTCCAAAAACCAAAGCAAGGTGGTAGATAAAGTG
CCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTCGTTGCCCAAAGAAAATCCGAGCTGCTGGT
ATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)

Bacterial type II and III secretion system protein (Secretin)

(278-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.863

98.871

0.691
  comQ Acinetobacter baylyi ADP1

43.556

100

0.442
  pilQ Acinetobacter baumannii strain A118

42.026

100

0.44
  pilQ Acinetobacter baumannii D1279779

42.026

100

0.44
  pilQ Vibrio campbellii strain DS40M4

44.681

95.485

0.427
  pilQ Legionella pneumophila strain Lp02

44.681

95.485

0.427
  pilQ Legionella pneumophila strain ERS1305867

44.681

95.485

0.427
  pilQ Vibrio cholerae strain A1552

45.215

94.357

0.427
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

45.215

94.357

0.427

Multiple sequence alignment