Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GBK02_RS05205 Genome accession   NZ_CP045202
Coordinates   1064307..1066022 (-) Length   571 a.a.
NCBI ID   WP_203468688.1    Uniprot ID   A0A974SUS1
Organism   Dechloromonas sp. TW-R-39-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1059307..1071022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBK02_RS05180 (GBK02_05160) aspS 1059461..1061260 (-) 1800 WP_203468684.1 aspartate--tRNA ligase -
  GBK02_RS05185 (GBK02_05165) - 1061278..1061892 (-) 615 WP_203469305.1 DUF502 domain-containing protein -
  GBK02_RS05190 (GBK02_05170) - 1061892..1062146 (-) 255 WP_203468685.1 FmdB family zinc ribbon protein -
  GBK02_RS05195 (GBK02_05175) pilD 1062217..1063065 (-) 849 WP_203468686.1 A24 family peptidase Machinery gene
  GBK02_RS05200 (GBK02_05180) pilC 1063065..1064291 (-) 1227 WP_203468687.1 type II secretion system F family protein Machinery gene
  GBK02_RS05205 (GBK02_05185) pilB 1064307..1066022 (-) 1716 WP_203468688.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GBK02_RS05210 (GBK02_05190) - 1066101..1067348 (-) 1248 WP_239003179.1 HlyC/CorC family transporter -
  GBK02_RS05215 (GBK02_05195) - 1067376..1068215 (-) 840 WP_203468690.1 inner membrane protein YpjD -
  GBK02_RS05220 (GBK02_05200) ffh 1068245..1069603 (+) 1359 WP_203468691.1 signal recognition particle protein -
  GBK02_RS05225 (GBK02_05205) - 1069604..1070215 (-) 612 WP_203468692.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62068.67 Da        Isoelectric Point: 5.7925

>NTDB_id=392661 GBK02_RS05205 WP_203468688.1 1064307..1066022(-) (pilB) [Dechloromonas sp. TW-R-39-2]
MAATPQTQPLSGLARALVQAGRLKEAEAEQLLAQANTEKHSLIEQIIASQKASAIEIARFVADTFGYPLLDLAAFDEAHI
PETAIDRKLIATHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEHPKLAPLVAKYSESAADTLKNLASD
DINLDFLDEESSAKADEAAGQEIDDAPVVKFIQKMLLDAINEGASDIHFEPYEKYYRIRFRVDGILREIATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMRLVLSKNRAIDFRVSTLPTLQGEKIVLRILDPTSATLGIEALGYEPEQKIALLDAVN
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGVNISTAEDPAEINLAGINQVNVDDKAGLTFPVALKAFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLMNMGVPTFNIASSILLITAQRLARRLCTCKKPMTVPEQAMLDAG
FKPEELDGSWTLYGPGGCDRCKGSGYKGRVGIYQVMPVSEAMQRLIMSGATALDLALLAKNEGVKDLRQSGLLKVKQGVT
SLDEVLTTTNL

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=392661 GBK02_RS05205 WP_203468688.1 1064307..1066022(-) (pilB) [Dechloromonas sp. TW-R-39-2]
ATGGCTGCAACTCCTCAAACACAACCCCTCAGTGGTCTGGCACGCGCGCTGGTACAAGCCGGACGACTCAAGGAGGCGGA
GGCAGAGCAACTGCTGGCCCAGGCGAATACTGAAAAACATTCGCTGATCGAACAAATCATTGCCAGCCAAAAGGCATCTG
CCATTGAGATCGCACGATTTGTTGCTGACACATTCGGCTATCCGCTACTGGATCTTGCTGCCTTCGACGAAGCACACATT
CCGGAAACAGCAATTGATCGCAAGTTAATTGCGACCCACAAAGTCATTCCGCTCAACAAACGCGGCAATCGTCTTTCGGT
TGCCATTGCCGACCCGACCAATTTACGCGCGCTGGACGAAATCCGCTTCCAGACTGGCCTGGCCGTCGACCCTATTGTTG
TAGAACACCCCAAGCTTGCTCCGCTTGTCGCGAAATACTCGGAGTCTGCTGCCGATACTTTAAAAAACCTGGCCAGCGAC
GACATCAATCTCGACTTCTTGGATGAAGAGAGCAGCGCCAAAGCAGACGAAGCCGCAGGGCAAGAAATCGACGACGCCCC
CGTCGTCAAATTCATCCAGAAAATGCTGCTTGATGCGATCAACGAAGGGGCGTCGGACATTCATTTCGAGCCCTACGAGA
AATACTACCGAATTCGCTTCCGGGTCGACGGCATTCTTCGCGAAATCGCCACCCCCCCGCTGGCCATCAAGGAAAAGATC
GCTTCGCGCATCAAGGTCATTTCGCGTTTGAATATTGCAGAAAAGCGCGTCCCGCAAGACGGCCGCATGCGCTTGGTCCT
GTCAAAAAATCGGGCCATCGATTTTCGGGTCAGTACGCTACCCACCCTTCAGGGTGAGAAAATCGTCTTGCGTATTCTCG
ACCCAACGTCTGCCACGCTTGGTATTGAGGCACTGGGTTATGAGCCGGAGCAGAAAATAGCTCTGCTTGATGCGGTCAAC
CGGCCATACGGCATGGTTTTGGTCACAGGTCCTACCGGCTCCGGCAAGACTGTCTCGCTGTACACCTGCCTGAACATCCT
CAACAAGGATGGCGTCAACATTTCTACGGCGGAGGATCCGGCCGAAATCAATTTGGCGGGCATCAATCAGGTCAATGTAG
ACGACAAAGCCGGACTCACCTTTCCTGTTGCCCTGAAAGCCTTCCTTCGCCAGGATCCGGACGTCATCATGGTGGGTGAA
ATCCGGGATCTCGAAACTGCTGAAATTGCAATCAAAGCAGCACAGACGGGGCACATGGTGCTCTCGACGCTACACACCAA
CGATGCGCCACAAACACTGACACGACTGATGAATATGGGTGTTCCCACATTCAACATCGCGTCGAGCATCTTGTTGATTA
CCGCACAACGCCTGGCACGCAGGCTTTGCACCTGCAAGAAACCCATGACCGTCCCCGAGCAGGCAATGCTCGATGCAGGC
TTTAAGCCGGAAGAGCTGGATGGTTCATGGACGCTTTATGGGCCGGGAGGCTGTGATCGCTGCAAAGGCAGCGGCTATAA
AGGCCGGGTCGGCATTTATCAGGTCATGCCAGTTTCGGAGGCCATGCAGCGCTTGATCATGAGCGGTGCCACGGCGCTGG
ATCTGGCCCTCCTGGCTAAAAATGAGGGGGTCAAGGACTTGCGTCAGTCCGGACTGCTCAAGGTCAAGCAAGGGGTTACC
TCGCTTGATGAAGTATTGACCACGACCAATCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.371

100

0.545
  pilB Acinetobacter baumannii D1279779

53.659

100

0.539
  pilB Legionella pneumophila strain ERS1305867

52.448

100

0.525
  pilF Neisseria gonorrhoeae MS11

51.237

99.124

0.508
  pilB Vibrio cholerae strain A1552

49.03

99.299

0.487
  pilB Vibrio campbellii strain DS40M4

48.663

98.249

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

48.233

99.124

0.478
  pilF Thermus thermophilus HB27

38.25

100

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.833

98.599

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

41.153

88.091

0.363

Multiple sequence alignment