Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GA646_RS13465 Genome accession   NZ_CP045127
Coordinates   2644263..2645969 (+) Length   568 a.a.
NCBI ID   WP_075167609.1    Uniprot ID   A0AAW8Z0C9
Organism   Acinetobacter indicus strain XG03     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2639263..2650969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA646_RS13425 nusA 2639931..2641415 (-) 1485 WP_005181786.1 transcription termination factor NusA -
  GA646_RS13430 rimP 2641448..2641972 (-) 525 WP_005181784.1 ribosome maturation factor RimP -
  GA646_RS13455 secG 2642841..2643170 (-) 330 WP_016659654.1 preprotein translocase subunit SecG -
  GA646_RS13460 tpiA 2643184..2643975 (-) 792 WP_160255746.1 triose-phosphate isomerase -
  GA646_RS13465 pilB 2644263..2645969 (+) 1707 WP_075167609.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GA646_RS13470 pilC 2646000..2647226 (+) 1227 WP_160255747.1 type II secretion system F family protein Machinery gene
  GA646_RS13475 pilD 2647226..2648086 (+) 861 WP_160255748.1 prepilin peptidase Machinery gene
  GA646_RS13480 coaE 2648097..2648705 (+) 609 WP_005181773.1 dephospho-CoA kinase -
  GA646_RS13485 - 2648702..2649622 (-) 921 WP_160255749.1 DMT family transporter -
  GA646_RS13490 rlmB 2649640..2650392 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GA646_RS13495 - 2650498..2650821 (-) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63787.16 Da        Isoelectric Point: 4.8301

>NTDB_id=392102 GA646_RS13465 WP_075167609.1 2644263..2645969(+) (pilB) [Acinetobacter indicus strain XG03]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAARKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDDDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=392102 GA646_RS13465 WP_075167609.1 2644263..2645969(+) (pilB) [Acinetobacter indicus strain XG03]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCAAGAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACTATTGCTGAAAAAATTTCCTATGAATTTGGTGAGCCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGGATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTGGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATGACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAATTACTAGTCGATGCAATCCGTATGGGCGCCTCGGATTTGCACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTTTTGCGCCAGATTGCCAATCCTCCGTTACAACTGGCCACACGCCTTGCCTCACGTTTA
AAAGTCATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCATTGCCGACCCTGTTTGGAGAAAAAATCGTACTACGTATTCTCGATCCATCTAGCG
CCATGCTGGGGATTGATGCACTTGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTACTGATTACCGGTCCGACCGGTTCCGGTAAAACCGTGTCTTTATATACCGGCTTAAATATCCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGGATTAATCAGGTCAACGTCAATCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGCCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCTATTAAAGCTGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCTGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCTTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTGTGTCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAACTCGGTTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTAATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGTAATGCCTTGCAAATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.806
  pilB Acinetobacter baylyi ADP1

77.895

100

0.782
  pilB Legionella pneumophila strain ERS1305867

50.97

99.824

0.509
  pilF Neisseria gonorrhoeae MS11

51.429

98.592

0.507
  pilB Vibrio cholerae strain A1552

49.647

99.648

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.372

98.063

0.484
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.434

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

33.659

100

0.364

Multiple sequence alignment