Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GA646_RS13475 Genome accession   NZ_CP045127
Coordinates   2647226..2648086 (+) Length   286 a.a.
NCBI ID   WP_160255748.1    Uniprot ID   A0AAW8Z0U2
Organism   Acinetobacter indicus strain XG03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2642226..2653086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA646_RS13455 secG 2642841..2643170 (-) 330 WP_016659654.1 preprotein translocase subunit SecG -
  GA646_RS13460 tpiA 2643184..2643975 (-) 792 WP_160255746.1 triose-phosphate isomerase -
  GA646_RS13465 pilB 2644263..2645969 (+) 1707 WP_075167609.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GA646_RS13470 pilC 2646000..2647226 (+) 1227 WP_160255747.1 type II secretion system F family protein Machinery gene
  GA646_RS13475 pilD 2647226..2648086 (+) 861 WP_160255748.1 prepilin peptidase Machinery gene
  GA646_RS13480 coaE 2648097..2648705 (+) 609 WP_005181773.1 dephospho-CoA kinase -
  GA646_RS13485 - 2648702..2649622 (-) 921 WP_160255749.1 DMT family transporter -
  GA646_RS13490 rlmB 2649640..2650392 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GA646_RS13495 - 2650498..2650821 (-) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32047.31 Da        Isoelectric Point: 7.5592

>NTDB_id=392104 GA646_RS13475 WP_160255748.1 2647226..2648086(+) (pilD) [Acinetobacter indicus strain XG03]
MQEFIHYLIQHPTALYFVVGIFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIMDESKISLSQPASTCPKCHSP
IHWYQNIPVISWLLLRGKCGQCQNPISIRYPFIELLTAVCALTVVAVFGPTLQMLFGLVLTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSASSAIWGYIIGFLCLWVVYYLFKLVTGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGDAIMKLYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=392104 GA646_RS13475 WP_160255748.1 2647226..2648086(+) (pilD) [Acinetobacter indicus strain XG03]
ATGCAAGAATTCATTCATTATCTGATTCAACACCCAACTGCGCTGTATTTCGTAGTTGGGATTTTTAGTTTATGTATTGG
CAGTTTTCTGAATGTGGTGATTTACCGCACGCCAAAAATGATGGAACAGGAATGGCGCAATGACTGTCAAATGCTGCTGC
ATCCTGAACAGGCCATCATGGATGAAAGTAAAATCAGCCTCAGTCAGCCAGCCTCGACCTGTCCGAAATGCCACAGCCCG
ATTCACTGGTATCAGAATATTCCAGTAATCAGCTGGCTGCTTCTACGTGGCAAATGTGGCCAATGCCAAAACCCGATCAG
TATTCGTTATCCTTTTATTGAACTGCTGACCGCAGTGTGTGCCTTAACCGTAGTCGCGGTTTTTGGCCCGACCCTGCAAA
TGCTGTTTGGCCTGGTGTTGACCTATGTGTTAATTGCCCTGACCTTTATTGATTTTGACACCCAGCTGCTGCCTGACCGC
TATACCCTCCCCCTGGCTGCACTCGGCTTGGGGGTGAACAGCTATGCCCTGTACACCTCCGCTAGCTCTGCCATTTGGGG
ATATATCATTGGCTTTTTATGTCTGTGGGTGGTGTATTACCTGTTTAAGCTGGTGACTGGCAAAGAAGGTATGGGCTATG
GCGATTTTAAATTGCTGGCCGCCTTAGGTGCCTGGATGGGCCCGCTGATGCTGCCGCTGATTGTGTTGCTATCTTCTCTG
GTTGGTGCAATCATTGGCATTATTTTGCTGAAAATCCGCAAAGAGAACCAGCCTTTTGCCTTTGGCCCTTATATTGCCAT
TGCCGGCTGGATCGCCTTTTTGTGGGGTGATGCAATCATGAAGCTTTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

79.298

99.65

0.79
  pilD Acinetobacter baumannii D1279779

78.947

99.65

0.787
  pilD Vibrio cholerae strain A1552

55.056

93.357

0.514
  pilD Vibrio campbellii strain DS40M4

52.809

93.357

0.493
  pilD Neisseria gonorrhoeae MS11

47.328

91.608

0.434

Multiple sequence alignment