Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GBN93_RS01575 Genome accession   NZ_CP045103
Coordinates   333441..335153 (-) Length   570 a.a.
NCBI ID   WP_234965190.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ413     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328441..340153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN93_RS01545 (GBN93_01560) - 328540..328863 (+) 324 WP_125280995.1 pyrimidine/purine nucleoside phosphorylase -
  GBN93_RS01550 (GBN93_01565) rlmB 329005..329754 (+) 750 WP_234965185.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GBN93_RS01555 (GBN93_01570) - 329787..330704 (+) 918 WP_234965186.1 DMT family transporter -
  GBN93_RS01560 (GBN93_01575) coaE 330718..331317 (-) 600 WP_234965187.1 dephospho-CoA kinase -
  GBN93_RS01565 (GBN93_01580) pilD 331324..332184 (-) 861 WP_234965188.1 prepilin peptidase Machinery gene
  GBN93_RS01570 (GBN93_01585) pilC 332184..333410 (-) 1227 WP_234965189.1 type II secretion system F family protein Machinery gene
  GBN93_RS01575 (GBN93_01590) pilB 333441..335153 (-) 1713 WP_234965190.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GBN93_RS01580 (GBN93_01595) tpiA 335433..336224 (+) 792 WP_234965191.1 triose-phosphate isomerase -
  GBN93_RS01585 (GBN93_01600) secG 336238..336567 (+) 330 WP_004692710.1 preprotein translocase subunit SecG -
  GBN93_RS01615 (GBN93_01630) rimP 337521..338045 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  GBN93_RS01620 (GBN93_01635) nusA 338085..339569 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63586.91 Da        Isoelectric Point: 5.0056

>NTDB_id=391942 GBN93_RS01575 WP_234965190.1 333441..335153(-) (pilB) [Acinetobacter johnsonii strain AJ413]
MSVSNGLPKFTGFIRRLVEDGRVTAENMQQAMLSAKKAQQDIVPYLIENYKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPNQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=391942 GBN93_RS01575 WP_234965190.1 333441..335153(-) (pilB) [Acinetobacter johnsonii strain AJ413]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTAATTGAAAATTATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGAGATCTGGTCGAAGACAAACTCATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCAATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCCAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCACCGATCGT
CAAATACATCAATAAATTACTGATTGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAAATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACATTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAAGATCCTGATATTGTAATGGTGGGTGAGATTCGC
GACCTTGAAACAGCTGAAATCGCAATTAAAGCCGCGCAAACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGTAATATGGGGGTTCCATCTTTTAACATTGCGACCTCGGTCAATTTAGTGATTGCAC
AGCGTTTGGCACGCCGTTTATGTAGCCAATGCAAAGCCGCTGTCGACATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTTATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCTGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.842

100

0.768
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.67

97.895

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.572

100

0.375
  pilF Thermus thermophilus HB27

36.602

100

0.367

Multiple sequence alignment