Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GBN68_RS01430 Genome accession   NZ_CP045101
Coordinates   305681..307393 (-) Length   570 a.a.
NCBI ID   WP_234955121.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ210     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 300681..312393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN68_RS01400 (GBN68_01400) - 300778..301101 (+) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -
  GBN68_RS01405 (GBN68_01405) rlmB 301244..301993 (+) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GBN68_RS01410 (GBN68_01410) - 302029..302944 (+) 916 Protein_265 DMT family transporter -
  GBN68_RS01415 (GBN68_01415) coaE 302958..303557 (-) 600 WP_234955114.1 dephospho-CoA kinase -
  GBN68_RS01420 (GBN68_01420) pilD 303564..304424 (-) 861 WP_234955116.1 prepilin peptidase Machinery gene
  GBN68_RS01425 (GBN68_01425) pilC 304424..305650 (-) 1227 WP_234955119.1 type II secretion system F family protein Machinery gene
  GBN68_RS01430 (GBN68_01430) pilB 305681..307393 (-) 1713 WP_234955121.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GBN68_RS01435 (GBN68_01435) tpiA 307673..308464 (+) 792 WP_234955123.1 triose-phosphate isomerase -
  GBN68_RS01440 (GBN68_01440) secG 308478..308807 (+) 330 WP_168159357.1 preprotein translocase subunit SecG -
  GBN68_RS01470 (GBN68_01470) rimP 309759..310283 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  GBN68_RS01475 (GBN68_01475) nusA 310323..311807 (+) 1485 WP_062035165.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63502.84 Da        Isoelectric Point: 5.1412

>NTDB_id=391900 GBN68_RS01430 WP_234955121.1 305681..307393(-) (pilB) [Acinetobacter johnsonii strain AJ210]
MSVSNGLPKFTGFIRRLVEDGRVTAENMQQAMLSAKKAQQDIVPYLIANHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLNLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=391900 GBN68_RS01430 WP_234955121.1 305681..307393(-) (pilB) [Acinetobacter johnsonii strain AJ210]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGCAAACCATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATTCTCTATAT
CGCTACCAGCAATCCAACCAACATGGATGCGATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCCCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCTGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAAACTTAGAGCAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCTCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCCTCAGACTTACACTTTGAGCCTTATGAAAAATCTT
ACCGTGTTCGTTACCGTGTAGATGGGGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGGATTAAATTAAAACTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCCACCTTGTTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAGGATCCTGATATTGTAATGGTGGGTGAGATTCGT
GACCTTGAAACGGCTGAAATCGCGATTAAAGCCGCACAGACAGGCCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACCCTGACACGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACCTCAGTCAATTTGGTAATTGCAC
AGCGTTTGGCACGCCGGTTATGTAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGATTTCAAAGTTTATCAACCGGTGGGATGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.053

100

0.811
  pilB Acinetobacter baylyi ADP1

76.667

100

0.767
  pilB Legionella pneumophila strain ERS1305867

53.745

93.684

0.504
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio cholerae strain A1552

48.75

98.246

0.479
  pilB Vibrio campbellii strain DS40M4

47.849

97.895

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

49.811

92.632

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.733

100

0.377
  pilF Thermus thermophilus HB27

36.602

100

0.367

Multiple sequence alignment