Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F9230_RS15625 Genome accession   NZ_CP045099
Coordinates   3263563..3265275 (+) Length   570 a.a.
NCBI ID   WP_168159359.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3258563..3270275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9230_RS15580 (F9230_15630) nusA 3259145..3260629 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  F9230_RS15585 (F9230_15635) rimP 3260669..3261193 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  F9230_RS15615 (F9230_15665) secG 3262149..3262478 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  F9230_RS15620 (F9230_15670) tpiA 3262492..3263283 (-) 792 WP_168159358.1 triose-phosphate isomerase -
  F9230_RS15625 (F9230_15675) pilB 3263563..3265275 (+) 1713 WP_168159359.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F9230_RS15630 (F9230_15680) pilC 3265306..3266532 (+) 1227 WP_159152946.1 type II secretion system F family protein Machinery gene
  F9230_RS15635 (F9230_15685) pilD 3266532..3267392 (+) 861 WP_218288793.1 prepilin peptidase Machinery gene
  F9230_RS15640 (F9230_15690) coaE 3267399..3267998 (+) 600 WP_224216451.1 dephospho-CoA kinase -
  F9230_RS15645 (F9230_15695) - 3268013..3268930 (-) 918 WP_029574926.1 DMT family transporter -
  F9230_RS15650 (F9230_15700) rlmB 3268963..3269712 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  F9230_RS15655 (F9230_15705) - 3269855..3270178 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63608.87 Da        Isoelectric Point: 4.8942

>NTDB_id=391877 F9230_RS15625 WP_168159359.1 3263563..3265275(+) (pilB) [Acinetobacter johnsonii strain AJ14]
MSVSNGLPKFTGFIRRLVEDGRVTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDPQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNQCKTAVDIPEQSLLEMGF
TSTDIQDPDFKVYQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=391877 F9230_RS15625 WP_168159359.1 3263563..3265275(+) (pilB) [Acinetobacter johnsonii strain AJ14]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAAGCTCAGCAAGACATCGTCCCTTACCTGATTGAAAATCATAAACTCTCTC
CACAAATGATCGCTGAGACGATTTCTTTAGAATTTGGCGAGCCCGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCTACCAGCAATCCAACCAATATGGATGCGATGGACGCAATTCGCTTCAATTCTAAAATGAATATTGAAGCAATTATTG
TTGAACATCCCCAGCTTGAAAAACTGATCGAACAAAATTTTGCGCAGGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAACAAGACCCTCAGAACTCCAATGACGAAGAAGACGATTCACCTCAAGGCGATGAAGCACCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGATTTACACTTTGAGCCTTATGAGAAATCTT
ACCGTGTCCGTTATCGTGTAGATGGGGTTCTGCGTCACATTGCCAGCCCACCACTACAACTGGCGACTCGTCTTGCTTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGTCGTATTAAATTAAAGCTGTCAAA
AAGTAAAGCCATTGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGTGAAAAATTGGTTCTGCGTATTCTCGATCCAT
CTAGTGCTATGCTAGGGATCGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTGTTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTGTCGTTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACTAATATCTCAACAGCGGAAGACCCTGTTGAAATTAACTTAGAAGGTATTAATCAGGTCAATGTAAACAATA
AAGTCGGCCTCACCTTTTCTGCTGCACTAAAGTCCTTCTTACGTCAGGACCCTGATATTGTGATGGTCGGTGAGATTCGC
GACCTTGAAACGGCTGAAATCGCAATTAAAGCTGCACAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACCCTGACACGCTTACGCAATATGGGGGTTCCCTCTTTTAACATTGCAACCTCGGTCAATTTGGTGATTGCAC
AACGTTTAGCGCGCCGCTTATGTAACCAATGCAAAACCGCTGTTGATATTCCCGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCAACAGATATCCAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTACTGAGTGCCGTGAAGGTTATAAAGG
GCGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCGTTGCAGA
TTGCTGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.842

100

0.768
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.849

97.895

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.41

100

0.374
  pilF Thermus thermophilus HB27

36.778

100

0.368

Multiple sequence alignment