Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GBN67_RS13925 Genome accession   NZ_CP045051
Coordinates   2880111..2881823 (+) Length   570 a.a.
NCBI ID   WP_234963132.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ312     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2875111..2886823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN67_RS13880 (GBN67_13895) nusA 2875695..2877179 (-) 1485 WP_183173596.1 transcription termination factor NusA -
  GBN67_RS13885 (GBN67_13900) rimP 2877219..2877743 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  GBN67_RS13915 (GBN67_13930) secG 2878697..2879026 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  GBN67_RS13920 (GBN67_13935) tpiA 2879040..2879831 (-) 792 WP_010325769.1 triose-phosphate isomerase -
  GBN67_RS13925 (GBN67_13940) pilB 2880111..2881823 (+) 1713 WP_234963132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GBN67_RS13930 (GBN67_13945) pilC 2881854..2883080 (+) 1227 WP_010325767.1 type II secretion system F family protein Machinery gene
  GBN67_RS13935 (GBN67_13950) pilD 2883080..2883940 (+) 861 WP_234963133.1 prepilin peptidase Machinery gene
  GBN67_RS13940 (GBN67_13955) coaE 2883947..2884546 (+) 600 WP_062035173.1 dephospho-CoA kinase -
  GBN67_RS13945 (GBN67_13960) - 2884561..2885478 (-) 918 WP_029574926.1 DMT family transporter -
  GBN67_RS13950 (GBN67_13965) rlmB 2885511..2886148 (-) 638 Protein_2703 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GBN67_RS13955 (GBN67_13970) - 2886290..2886613 (-) 324 WP_125280995.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63573.92 Da        Isoelectric Point: 5.0082

>NTDB_id=391542 GBN67_RS13925 WP_234963132.1 2880111..2881823(+) (pilB) [Acinetobacter johnsonii strain AJ312]
MSVSNGLPKFTGFIRRLVEDGRVTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSIDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=391542 GBN67_RS13925 WP_234963132.1 2880111..2881823(+) (pilB) [Acinetobacter johnsonii strain AJ312]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAACCATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATTCTCTATAT
CGCTACCAGCAATCCAACCAACATGGATGCAATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCCAATGACGAAGAAGATGACTCGCCTCAAGGGGATGAAGCACCGATCGT
CAAATACATCAATAAATTACTGATTGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGAGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAAGATCCTGATATTGTGATGGTGGGTGAGATTCGC
GACCTTGAAACAGCTGAAATCGCAATTAAAGCTGCACAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGTAATATGGGGGTTCCATCTTTTAACATTGCGACCTCGGTCAATTTAGTGATTGCAC
AGCGTTTGGCACGCCGTTTATGTAGCCAATGCAAAGCCGCTGTCGACATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCATAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTTATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCTGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.404

100

0.814
  pilB Acinetobacter baylyi ADP1

76.842

100

0.768
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.67

97.895

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

50.87

90.702

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.572

100

0.375
  pilF Thermus thermophilus HB27

36.602

100

0.367

Multiple sequence alignment