Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FSC10_RS01500 Genome accession   NZ_CP044463
Coordinates   306227..307936 (-) Length   569 a.a.
NCBI ID   WP_163170779.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain HZE23-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 301227..312936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC10_RS01470 (FSC10_01495) - 301311..301634 (+) 324 WP_004810087.1 pyrimidine/purine nucleoside phosphorylase -
  FSC10_RS01475 (FSC10_01500) rlmB 301787..302536 (+) 750 WP_004810085.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FSC10_RS01480 (FSC10_01505) - 302543..303466 (+) 924 WP_163170776.1 DMT family transporter -
  FSC10_RS01485 (FSC10_01510) coaE 303481..304080 (-) 600 WP_163164686.1 dephospho-CoA kinase -
  FSC10_RS16630 (FSC10_01515) pilD 304082..304945 (-) 864 WP_163170777.1 prepilin peptidase Machinery gene
  FSC10_RS01495 (FSC10_01520) pilC 304963..306195 (-) 1233 WP_163170778.1 type II secretion system F family protein Machinery gene
  FSC10_RS01500 (FSC10_01525) pilB 306227..307936 (-) 1710 WP_163170779.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FSC10_RS01505 (FSC10_01530) tpiA 308219..309010 (+) 792 WP_163170780.1 triose-phosphate isomerase -
  FSC10_RS01510 (FSC10_01535) secG 309024..309353 (+) 330 WP_004810073.1 preprotein translocase subunit SecG -
  FSC10_RS01540 (FSC10_01565) rimP 310415..310939 (+) 525 WP_163164694.1 ribosome maturation factor RimP -
  FSC10_RS01545 (FSC10_01570) nusA 310975..312459 (+) 1485 WP_004810069.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63494.71 Da        Isoelectric Point: 4.9211

>NTDB_id=390156 FSC10_RS01500 WP_163170779.1 306227..307936(-) (pilB) [Acinetobacter schindleri strain HZE23-1]
MTAIQGSPRFTGFIRRLVDEGVISAEDMQGALSSAKKDKIDIVPYLIEHHRLSPTKIAETISLEFGEPLFDISAYDPALI
VREDFDEKIITKHRVLPIFKNSNILFIATSNPTNIEAIDAVRFATKLNIETIIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDIDGSNANQDTEEENKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLATRLASR
LKVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKKPADIPRQSLLEMGFS
DDDLQNPEFNIFEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLLKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=390156 FSC10_RS01500 WP_163170779.1 306227..307936(-) (pilB) [Acinetobacter schindleri strain HZE23-1]
ATGACAGCAATTCAGGGATCTCCAAGATTTACGGGGTTTATTCGTCGACTTGTCGATGAAGGGGTTATATCTGCAGAAGA
TATGCAGGGTGCTTTGAGCAGTGCCAAAAAAGATAAAATTGATATTGTTCCCTATCTGATTGAACATCACCGCCTCTCGC
CAACCAAAATCGCAGAAACGATTTCTCTTGAATTCGGTGAACCGCTATTTGATATCAGTGCCTATGATCCTGCCCTGATC
GTACGCGAAGATTTTGATGAAAAGATCATTACCAAACACCGCGTGTTGCCAATCTTTAAAAATAGCAATATTCTGTTTAT
CGCGACCAGCAACCCGACCAATATAGAAGCAATTGATGCCGTTCGTTTTGCGACCAAACTCAATATTGAAACCATCATTG
TTGAACATAATAAACTCGAAAAACTGATCGAGCAGAATTTTACTGAAGAAAGCACCTTTGACTTTGATGATGATTTTGAT
CTAGATGTCGATATTGACGGTTCAAATGCCAATCAAGACACTGAAGAAGAAAATAAAGGTGATGAAGCACCAATTGTTAA
ATATATTAATAAGTTACTGATTGATGCAATCCGTATGGGTGCATCTGATTTACACTTTGAACCCTATGAAAAAATGTACC
GTGTGCGTTACCGTGTCGACGGGGTCTTGCGTCAAATCGCCACGCCACCTTTACAGCTTGCAACACGTCTGGCTTCACGT
CTTAAAGTTATGTCACAAATGGATATTTCCGAGAAGCGTATACCTCAAGATGGCCGGATCAAGTTAAAACTCTCTAAAAA
TAAGGCCATCGATTTCCGTGTCAACTCACTCCCCACCCTGTTTGGGGAAAAACTGGTACTGCGTATTCTGGATCCATCGA
GTGCCATGCTGGGTATTGATGCACTGGGGTATGAGCCTGATCAAAAAGCCTTGTTTATGGAAGCATTAGACAAACCACAG
GGCATGCTCTTGATCACCGGTCCGACAGGCTCTGGTAAAACCGTATCTCTATATACCGGTTTAAATATCCTGAATCGTGA
AGATACCAATATTTCAACTGCTGAAGACCCCGTTGAAATTAACCTGCAAGGGATCAACCAGGTTAACGTCAATAATAAAG
TCGGCCTAACCTTCTCTGCTGCCTTGAAATCATTCCTGCGTCAGGACCCTGATATTGTCATGGTCGGTGAGATCCGTGAC
CTGGAAACTGCAGAGATTGCGATCAAGGCAGCACAAACAGGTCATATGGTGATGTCAACACTACATACCAACAGCGCACC
AGAAACCTTGACCCGTTTACGGAATATGGGTGTGCCATCATTTAATATTGCCACCTCGGTGAATTTGGTGATCGCTCAGC
GTTTGGCGCGTCGCCTTTGTCCACATTGCAAAAAACCGGCAGATATTCCACGTCAGAGCCTGCTTGAAATGGGCTTTAGC
GATGATGATTTACAGAATCCGGAATTTAATATTTTTGAACCTGTCGGATGTAACGAATGTCGCGAAGGCTACAAAGGTCG
AGTCGGTATTTATGAAGTCATGAAAGTGACACCAGAGATTTCACGTATTATTATGGAAGATGGCAATGCAATTGAGATTG
CTGAAGCTTCCGCACGCGCAGGTTTTAACAATTTACGCCGATCAGGGTTATTAAAGGTAATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGTGTTACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80

100

0.801
  pilB Acinetobacter baylyi ADP1

75.965

100

0.761
  pilB Legionella pneumophila strain ERS1305867

52.037

94.903

0.494
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.372

97.891

0.483
  pilB Vibrio cholerae strain A1552

47.972

99.649

0.478
  pilB Vibrio campbellii strain DS40M4

48.282

97.188

0.469
  pilF Thermus thermophilus HB27

37.522

100

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

34.967

100

0.376

Multiple sequence alignment