Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FSC05_RS01395 Genome accession   NZ_CP044450
Coordinates   300952..303096 (+) Length   714 a.a.
NCBI ID   WP_163143400.1    Uniprot ID   -
Organism   Acinetobacter indicus strain MMS9-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 295952..308096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC05_RS01365 (FSC05_01375) - 296074..296688 (+) 615 WP_075167936.1 IS607 family transposase -
  FSC05_RS01370 (FSC05_01380) - 296685..297842 (+) 1158 WP_163140776.1 RNA-guided endonuclease InsQ/TnpB family protein -
  FSC05_RS01375 (FSC05_01385) pilM 297991..299013 (+) 1023 WP_163143396.1 pilus assembly protein PilM Machinery gene
  FSC05_RS01380 (FSC05_01390) pilN 299013..299648 (+) 636 WP_005180516.1 PilN domain-containing protein Machinery gene
  FSC05_RS01385 (FSC05_01395) pilO 299645..300364 (+) 720 WP_005180513.1 type 4a pilus biogenesis protein PilO Machinery gene
  FSC05_RS01390 (FSC05_01400) pilP 300364..300891 (+) 528 WP_045795346.1 pilus assembly protein PilP Machinery gene
  FSC05_RS01395 (FSC05_01405) pilQ 300952..303096 (+) 2145 WP_163143400.1 type IV pilus secretin PilQ Machinery gene
  FSC05_RS01400 (FSC05_01410) aroK 303133..303675 (+) 543 WP_075167858.1 shikimate kinase AroK -
  FSC05_RS01405 (FSC05_01415) aroB 303698..304780 (+) 1083 WP_160232278.1 3-dehydroquinate synthase -
  FSC05_RS01410 (FSC05_01420) - 304793..305629 (+) 837 WP_160232279.1 hypothetical protein -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77476.71 Da        Isoelectric Point: 4.8968

>NTDB_id=390071 FSC05_RS01395 WP_163143400.1 300952..303096(+) (pilQ) [Acinetobacter indicus strain MMS9-2]
MNNLFRQFSMGAVAMAVMQVANAQVSITNIVPMQIPGQGTEIRVMFNGLPPQPAAYQLEQPSRLVLDFDKAQQSLKQASI
PVATREASSVDVSSDAQRARLTVNLAEAGAFTTRVEGNTFILKINSTQAAATPVAVQQTAQGISNIGFQRGAEGEGQVVI
DLAGSNTPVDVQQQGSKIIVRTLGNKIPAHLARRLNVNDFATPVSTIDAQNDGGNGVITIQSAGSYEYMAYQAENKLTIS
LKRPVDKNPFKPRAAQSYTGKKISLDFQDIEVRRVLQLLADFTGINMVAADTVQGNITLRLKDVPWDQALDIILKTKNLD
KRRNGNVIWIAPVAELIKAEEEEAKAIAQSVKLAPLQTEYIQLNYAKAADIEKLITEGRNSKNSNNNSNNNSSTNNIDPL
GDSVGSLLSPRGTVSVDPRTNTLIINDTAQKIDQIRGMIDLLDVQVKQVMVEARVVRASTEFAKEMGVKWGILSQGITNN
NHLLVGGSDTTLWDLRTAEIDGTTGIPTYDIQRPDNLNVDLGVENAAGRIAFGLISLSDFMLDLELSALQADGYGEVIST
PKVLTADKQKATVESGLEVPYQSTEGSGANATATTEFKDVVLKLDVTPSITPDGKVQMDLDISSDSIAGVTPMGEYYLNK
NRLNTNVVVENGETVVLGGIFEQETLNQQTKVPFLGDIPYLGRLFRKDIKSDNKRELLIFVTPRIVNDSVSRNH

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=390071 FSC05_RS01395 WP_163143400.1 300952..303096(+) (pilQ) [Acinetobacter indicus strain MMS9-2]
ATGAACAATTTGTTCCGTCAGTTTTCAATGGGCGCTGTTGCGATGGCCGTCATGCAAGTGGCCAATGCGCAGGTCAGCAT
TACCAATATTGTACCAATGCAAATTCCAGGACAAGGTACTGAAATTCGTGTCATGTTCAATGGCTTGCCACCTCAGCCTG
CAGCCTATCAGCTTGAGCAGCCATCACGTTTGGTGCTGGATTTTGATAAAGCCCAGCAGAGCTTAAAACAGGCCAGCATT
CCGGTTGCGACCCGGGAAGCCAGCTCGGTTGATGTCAGTTCCGATGCCCAGCGTGCACGTTTAACGGTTAATCTGGCTGA
AGCGGGTGCTTTTACTACCCGTGTCGAAGGTAATACTTTTATTCTTAAAATTAACAGCACTCAGGCAGCCGCAACACCAG
TAGCTGTACAGCAGACGGCTCAAGGCATATCGAATATCGGCTTTCAGCGTGGTGCAGAAGGGGAAGGTCAGGTGGTAATT
GACCTGGCAGGCAGCAATACCCCGGTCGATGTGCAACAGCAAGGCAGTAAAATTATTGTCCGTACTTTGGGCAATAAAAT
TCCTGCGCATCTGGCACGCCGTTTAAACGTCAATGATTTTGCCACGCCTGTATCTACCATTGATGCGCAAAATGATGGTG
GCAATGGCGTAATTACCATTCAGTCGGCGGGAAGCTATGAATATATGGCGTATCAGGCGGAAAATAAACTGACCATTAGC
CTGAAACGTCCGGTAGATAAGAACCCGTTCAAACCGCGTGCAGCACAAAGCTATACCGGTAAAAAGATTTCTTTAGATTT
CCAGGATATTGAAGTGCGTCGTGTGCTGCAGTTACTGGCTGATTTTACCGGAATTAACATGGTTGCCGCCGATACGGTAC
AAGGCAATATCACCTTACGTCTCAAAGATGTGCCGTGGGATCAGGCACTAGATATTATTTTAAAAACCAAAAATCTGGAT
AAACGCCGTAATGGCAATGTGATCTGGATTGCACCCGTCGCAGAGCTGATCAAGGCTGAAGAAGAAGAAGCTAAAGCGAT
TGCACAAAGCGTCAAGCTGGCGCCGCTGCAAACCGAATATATCCAGTTAAATTATGCCAAGGCTGCTGATATTGAAAAAC
TGATTACTGAAGGACGTAATTCAAAAAATAGTAATAATAATTCCAATAACAATAGTAGTACTAATAATATAGATCCTTTG
GGTGATAGTGTTGGTAGTCTGCTCAGCCCACGTGGTACGGTGTCGGTTGATCCACGGACCAATACTTTGATTATTAATGA
TACTGCGCAGAAGATTGATCAGATTCGTGGCATGATTGACTTGCTGGATGTACAGGTCAAACAGGTCATGGTTGAGGCAC
GGGTTGTACGTGCATCAACAGAGTTTGCTAAAGAGATGGGGGTGAAATGGGGGATATTATCTCAAGGTATAACCAATAAC
AACCATTTGCTTGTAGGTGGTAGTGATACAACGCTCTGGGATTTAAGAACCGCTGAAATTGATGGCACTACAGGAATTCC
AACTTACGATATTCAGCGTCCAGATAACTTAAATGTTGATTTGGGTGTAGAGAATGCAGCGGGTCGTATTGCATTTGGTT
TGATTAGTTTATCTGACTTTATGCTTGATCTTGAATTGTCAGCACTTCAAGCAGATGGTTATGGCGAGGTTATTTCAACG
CCTAAAGTCCTAACAGCGGATAAACAAAAGGCGACAGTAGAGTCTGGTTTGGAAGTGCCATATCAATCTACCGAAGGTTC
GGGGGCTAATGCAACGGCTACAACTGAATTTAAAGATGTTGTATTAAAGCTGGATGTCACACCAAGTATTACACCTGATG
GTAAAGTGCAAATGGATTTGGATATATCGAGTGACAGTATCGCAGGGGTAACACCGATGGGTGAGTATTACCTGAATAAA
AATCGTTTAAATACGAATGTGGTGGTTGAAAATGGCGAAACTGTTGTTCTTGGTGGTATTTTTGAACAGGAAACACTAAA
TCAGCAAACTAAAGTGCCATTCCTGGGTGATATTCCGTATTTAGGCCGTTTATTCCGTAAAGATATTAAATCGGACAATA
AACGTGAGTTGCTGATTTTTGTGACACCACGAATTGTTAATGACAGTGTTTCAAGAAATCATTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(359-448)

Secretin and TonB N terminus short domain (STN)

(290-332)

Bacterial type II and III secretion system protein (Secretin)

(548-706)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

76.838

100

0.776
  pilQ Acinetobacter baumannii strain A118

76.699

100

0.775
  comQ Acinetobacter baylyi ADP1

74.198

100

0.745
  pilQ Pseudomonas aeruginosa PAK

39.689

99.16

0.394
  pilQ Legionella pneumophila strain Lp02

37.712

99.16

0.374
  pilQ Legionella pneumophila strain ERS1305867

37.712

99.16

0.374

Multiple sequence alignment