Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   FSC05_RS01385 Genome accession   NZ_CP044450
Coordinates   299645..300364 (+) Length   239 a.a.
NCBI ID   WP_005180513.1    Uniprot ID   N9KYM2
Organism   Acinetobacter indicus strain MMS9-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 294645..305364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC05_RS01365 (FSC05_01375) - 296074..296688 (+) 615 WP_075167936.1 IS607 family transposase -
  FSC05_RS01370 (FSC05_01380) - 296685..297842 (+) 1158 WP_163140776.1 RNA-guided endonuclease InsQ/TnpB family protein -
  FSC05_RS01375 (FSC05_01385) pilM 297991..299013 (+) 1023 WP_163143396.1 pilus assembly protein PilM Machinery gene
  FSC05_RS01380 (FSC05_01390) pilN 299013..299648 (+) 636 WP_005180516.1 PilN domain-containing protein Machinery gene
  FSC05_RS01385 (FSC05_01395) pilO 299645..300364 (+) 720 WP_005180513.1 type 4a pilus biogenesis protein PilO Machinery gene
  FSC05_RS01390 (FSC05_01400) pilP 300364..300891 (+) 528 WP_045795346.1 pilus assembly protein PilP Machinery gene
  FSC05_RS01395 (FSC05_01405) pilQ 300952..303096 (+) 2145 WP_163143400.1 type IV pilus secretin PilQ Machinery gene
  FSC05_RS01400 (FSC05_01410) aroK 303133..303675 (+) 543 WP_075167858.1 shikimate kinase AroK -
  FSC05_RS01405 (FSC05_01415) aroB 303698..304780 (+) 1083 WP_160232278.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 239 a.a.        Molecular weight: 27087.71 Da        Isoelectric Point: 5.1311

>NTDB_id=390069 FSC05_RS01385 WP_005180513.1 299645..300364(+) (pilO) [Acinetobacter indicus strain MMS9-2]
MSLDQYEEMNQDPVAAPKKKMTLDKFLQQWNTLDPNNYGSWPFSVKLTIWIFIIILIAFLGYFLAIKPKLDAISNARAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLQQLPKETEIPGLVEDVNVTGVNSGLRFKNIRLENEIKQEFFIEQP
ISIEATGDYHAFGSFVSGTAALPRIVTMHDFTITAGENKEKKTDIPQITYVAKAKTYRYVGNTDAQAGNSTTPATGAAQ

Nucleotide


Download         Length: 720 bp        

>NTDB_id=390069 FSC05_RS01385 WP_005180513.1 299645..300364(+) (pilO) [Acinetobacter indicus strain MMS9-2]
ATGAGTCTCGATCAATATGAAGAAATGAATCAGGATCCTGTTGCTGCGCCAAAGAAAAAAATGACGCTGGATAAATTCCT
GCAGCAATGGAATACCTTAGATCCGAATAACTATGGCAGCTGGCCGTTTTCCGTCAAGCTGACCATCTGGATCTTTATCA
TTATTTTAATTGCCTTCCTGGGCTATTTCCTGGCCATCAAGCCGAAGCTGGATGCAATTTCCAATGCGCGTGCACAAGAG
CAGAACCTGCTGAATGAGTTTCGTGAAAAAGATTCCAAACTGCGTAACTTGCAGCAATATCAGGCGCAGTTACAGGAAAT
GGAAGCCAACTTTAACCAGCAGCTACAACAGTTGCCAAAAGAAACCGAAATTCCAGGTCTGGTTGAAGATGTGAACGTTA
CGGGTGTGAACTCAGGCCTAAGATTTAAAAATATCCGTCTGGAAAATGAAATCAAGCAAGAGTTCTTTATCGAGCAGCCA
ATTTCGATTGAAGCCACCGGTGATTATCACGCTTTTGGTTCTTTTGTGAGTGGTACAGCTGCTTTACCGCGAATTGTGAC
CATGCATGATTTCACTATTACTGCTGGTGAAAACAAGGAAAAGAAAACCGATATTCCACAAATTACCTATGTCGCGAAAG
CCAAGACCTATCGCTATGTCGGGAATACCGATGCACAGGCCGGTAATAGTACGACTCCAGCCACAGGAGCAGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9KYM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

69.748

99.582

0.695
  comO Acinetobacter baylyi ADP1

67.213

100

0.686

Multiple sequence alignment