Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FR932_RS19285 Genome accession   NZ_CP044399
Coordinates   4272176..4273879 (-) Length   567 a.a.
NCBI ID   WP_019442597.1    Uniprot ID   A0A5J6WS90
Organism   Moritella marina ATCC 15381 strain MP-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4267176..4278879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR932_RS19260 (FR932_19265) mutT 4268471..4268863 (+) 393 WP_019442602.1 8-oxo-dGTP diphosphatase MutT -
  FR932_RS19265 (FR932_19270) yacG 4268910..4269095 (-) 186 WP_019442601.1 DNA gyrase inhibitor YacG -
  FR932_RS19270 (FR932_19275) coaE 4269129..4269734 (-) 606 WP_019442600.1 dephospho-CoA kinase -
  FR932_RS19275 (FR932_19280) pilD 4269784..4270674 (-) 891 WP_019442599.1 A24 family peptidase Machinery gene
  FR932_RS19280 (FR932_19285) pilC 4270791..4272044 (-) 1254 WP_019442598.1 type II secretion system F family protein Machinery gene
  FR932_RS19285 (FR932_19290) pilB 4272176..4273879 (-) 1704 WP_019442597.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FR932_RS19290 (FR932_19295) - 4273886..4274332 (-) 447 WP_019442596.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FR932_RS19295 (FR932_19300) nadC 4274756..4275598 (-) 843 WP_019442595.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FR932_RS19300 (FR932_19305) - 4275623..4276129 (-) 507 WP_019442594.1 retropepsin-like aspartic protease -
  FR932_RS19305 (FR932_19310) ampD 4276306..4276872 (+) 567 WP_019442593.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FR932_RS19310 (FR932_19315) ampE 4276884..4277732 (+) 849 WP_019442592.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62861.20 Da        Isoelectric Point: 5.2006

>NTDB_id=389760 FR932_RS19285 WP_019442597.1 4272176..4273879(-) (pilB) [Moritella marina ATCC 15381 strain MP-1]
MQHKHNASGLAHSLVAHAYLAVEEIPQLLQSAKLEGKAFTTLLVDSNIIDSQVLAKLLEREYGVPLLDLDTFKLEEIPSN
LLNEKLIEKHHALPIYVQGQTLYLAMSDPTNVSALEEFAFSFSLHTEVLLVDELQLQKALESVLENDIDALGDLNDTDID
DLEVDNSESRLDQENKDSNDDAPIVKFVNKILLDAIKKGASDIHFEPYEFKYRVRFRIDGILHEMVSPPVNLAMRFSARL
KVMAQLDIAERRIPQDGRIKLKLSKNKSVDLRVSTLPTMWGEKVVMRILDSNQASLNIDILGYDNKQKDLYLKALQKPQG
MILITGPTGSGKTVSLYSGLNILNTVERNISTAEDPIEINLSGINQVQINLKAGLTFPTALRSFLRQDPDVVMVGEIRDI
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLSNMGVPAYNIGSSVSLIIAQRLGRRLCNECKQPEDIPAFELAKLGFNQ
QQIDLGITPFKAIGCKQCTKGYKGRVGIYEVMPMSDKIARMILTGSNSLELGSQAQQEGMDTLRQSALQKVIDGVTSLLE
VERITSH

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=389760 FR932_RS19285 WP_019442597.1 4272176..4273879(-) (pilB) [Moritella marina ATCC 15381 strain MP-1]
ATGCAACACAAGCATAACGCGAGCGGCCTGGCACACAGCCTGGTCGCCCATGCATACCTCGCTGTAGAAGAGATCCCCCA
GCTTCTGCAAAGCGCTAAACTGGAAGGTAAAGCCTTCACAACCCTACTCGTCGACAGTAATATCATCGACAGTCAGGTAT
TAGCCAAATTACTCGAGCGTGAATACGGTGTGCCCTTACTTGATTTAGATACCTTTAAGCTCGAAGAAATCCCCAGCAAC
CTGCTCAATGAAAAACTCATCGAAAAACACCATGCACTGCCTATCTATGTGCAAGGACAAACCTTATATCTGGCCATGTC
CGATCCGACTAATGTCAGCGCATTAGAAGAATTTGCCTTTAGCTTTAGCTTACATACCGAAGTATTACTGGTTGATGAAC
TGCAATTACAAAAAGCCTTAGAAAGCGTATTAGAAAATGACATTGATGCCCTAGGCGATCTTAACGATACCGATATCGAT
GACTTGGAAGTTGATAATAGCGAATCACGCTTAGATCAAGAAAATAAAGACAGTAATGACGATGCCCCGATTGTTAAATT
TGTGAATAAGATCCTCTTGGATGCGATCAAAAAAGGCGCATCCGACATTCACTTTGAACCCTATGAATTTAAATACCGTG
TACGTTTTCGTATCGATGGGATCTTACATGAAATGGTATCGCCACCGGTAAACTTAGCCATGCGTTTTTCAGCGCGGTTA
AAAGTCATGGCACAACTTGATATCGCCGAACGGCGGATCCCGCAAGATGGCCGTATCAAATTAAAATTATCGAAAAACAA
ATCGGTTGATCTACGCGTTAGCACCCTGCCAACGATGTGGGGTGAAAAAGTGGTAATGCGGATCTTAGATTCTAACCAAG
CTAGCCTCAATATCGATATTCTTGGTTATGACAATAAGCAAAAAGATCTTTACCTTAAAGCATTACAAAAGCCACAAGGC
ATGATCTTGATCACAGGCCCCACAGGTAGTGGTAAAACAGTTTCACTTTACAGTGGACTGAATATTCTCAACACCGTTGA
GCGCAACATCTCCACTGCCGAGGATCCGATCGAAATCAACCTCTCCGGTATCAACCAGGTACAAATCAACCTAAAAGCTG
GATTGACCTTCCCCACCGCATTGCGATCATTTTTACGTCAAGATCCAGATGTGGTGATGGTCGGTGAGATCCGCGATATT
GAAACAGCAGAGATTGCCATCAAAGCGGCGCAAACCGGTCACTTAGTCTTATCAACATTACATACCAACTCCGCTGCAGA
GACACTAACGCGTTTATCAAATATGGGGGTACCTGCTTACAACATTGGTTCGTCCGTGAGTTTGATCATCGCTCAGCGCC
TAGGTCGTCGACTCTGTAATGAATGCAAACAACCTGAAGATATTCCAGCGTTCGAATTAGCTAAACTTGGTTTTAACCAA
CAGCAGATTGATCTAGGTATTACGCCGTTTAAAGCGATCGGCTGTAAGCAATGTACCAAAGGTTATAAAGGGCGCGTGGG
CATCTACGAAGTAATGCCGATGTCAGATAAGATCGCCCGCATGATCTTAACCGGCAGCAACTCCTTGGAACTCGGGTCGC
AAGCTCAGCAAGAAGGCATGGATACCTTACGCCAATCAGCACTGCAAAAAGTCATCGACGGCGTCACTAGCTTGCTCGAA
GTCGAACGTATTACCAGTCATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6WS90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.465

100

0.526
  pilB Acinetobacter baumannii D1279779

52.575

99.295

0.522
  pilB Acinetobacter baylyi ADP1

52.313

99.118

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

50.98

98.942

0.504
  pilB Vibrio cholerae strain A1552

52.533

94.004

0.494
  pilB Vibrio campbellii strain DS40M4

51.287

95.944

0.492
  pilF Neisseria gonorrhoeae MS11

46.786

98.765

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

37.52

100

0.406
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.011

99.295

0.377
  pilF Thermus thermophilus HB27

36.852

98.589

0.363

Multiple sequence alignment