Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HHJ43_RS19360 Genome accession   NZ_CP051751
Coordinates   4049132..4050517 (+) Length   461 a.a.
NCBI ID   WP_024248108.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-301     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4044132..4055517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ43_RS19335 (HHJ43_19340) aroP 4044679..4046052 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  HHJ43_RS19340 (HHJ43_19345) ampE 4046095..4046949 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  HHJ43_RS19345 (HHJ43_19350) ampD 4046946..4047497 (-) 552 WP_096986659.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HHJ43_RS19350 (HHJ43_19355) nadC 4047585..4048478 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HHJ43_RS19355 (HHJ43_19360) pilA 4048682..4049122 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  HHJ43_RS19360 (HHJ43_19365) pilB 4049132..4050517 (+) 1386 WP_024248108.1 type II secretion system protein GspE Machinery gene
  HHJ43_RS19365 (HHJ43_19370) hofC 4050507..4051709 (+) 1203 WP_096986660.1 protein transport protein HofC -
  HHJ43_RS19370 (HHJ43_19375) guaC 4051744..4052787 (-) 1044 WP_096986661.1 GMP reductase -
  HHJ43_RS24135 - 4052943..4052987 (-) 45 WP_120795372.1 protein YacM -
  HHJ43_RS19375 (HHJ43_19380) coaE 4053012..4053632 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  HHJ43_RS19380 (HHJ43_19385) zapD 4053632..4054375 (+) 744 WP_001194731.1 cell division protein ZapD -
  HHJ43_RS19385 (HHJ43_19390) yacG 4054385..4054582 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  HHJ43_RS19390 (HHJ43_19395) mutT 4054671..4055069 (-) 399 WP_089574228.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50692.38 Da        Isoelectric Point: 6.4158

>NTDB_id=387948 HHJ43_RS19360 WP_024248108.1 4049132..4050517(+) (pilB) [Escherichia coli strain SCU-301]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQEEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLEMHARQAGMHTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=387948 HHJ43_RS19360 WP_024248108.1 4049132..4050517(+) (pilB) [Escherichia coli strain SCU-301]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTAGAACGTTACAGTCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAATCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCATTGCAACAAC
CACAGGGATTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATAAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCTGCGAAACGCTGGTGCGTTTACAGCAAATGGGCGTTGCTCGCTGGATGCTTTCATCGGCACTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGAGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAATGCACGCCCGACA
GGCGGGTATGCATACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Legionella pneumophila strain ERS1305867

49.738

82.863

0.412
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.312

83.514

0.403
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

84.599

0.395
  pilB Vibrio cholerae strain A1552

46.891

83.731

0.393
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilF Neisseria gonorrhoeae MS11

44.675

83.514

0.373
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Thermus thermophilus HB27

41.032

88.286

0.362