Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HHJ43_RS19355 Genome accession   NZ_CP051751
Coordinates   4048682..4049122 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain SCU-301     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4043682..4054122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ43_RS19335 (HHJ43_19340) aroP 4044679..4046052 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  HHJ43_RS19340 (HHJ43_19345) ampE 4046095..4046949 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  HHJ43_RS19345 (HHJ43_19350) ampD 4046946..4047497 (-) 552 WP_096986659.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HHJ43_RS19350 (HHJ43_19355) nadC 4047585..4048478 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HHJ43_RS19355 (HHJ43_19360) pilA 4048682..4049122 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  HHJ43_RS19360 (HHJ43_19365) pilB 4049132..4050517 (+) 1386 WP_024248108.1 type II secretion system protein GspE Machinery gene
  HHJ43_RS19365 (HHJ43_19370) hofC 4050507..4051709 (+) 1203 WP_096986660.1 protein transport protein HofC -
  HHJ43_RS19370 (HHJ43_19375) guaC 4051744..4052787 (-) 1044 WP_096986661.1 GMP reductase -
  HHJ43_RS24135 - 4052943..4052987 (-) 45 WP_120795372.1 protein YacM -
  HHJ43_RS19375 (HHJ43_19380) coaE 4053012..4053632 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=387947 HHJ43_RS19355 WP_000360914.1 4048682..4049122(+) (pilA) [Escherichia coli strain SCU-301]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=387947 HHJ43_RS19355 WP_000360914.1 4048682..4049122(+) (pilA) [Escherichia coli strain SCU-301]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGTATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363