Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOB45_RS20945 Genome accession   NZ_CP044086
Coordinates   2933021..2934706 (+) Length   561 a.a.
NCBI ID   WP_150347955.1    Uniprot ID   -
Organism   Pseudomonas luteola strain FDAARGOS_637     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2928021..2939706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB45_RS20925 (FOB45_20925) ampD 2928619..2929179 (-) 561 WP_150347952.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FOB45_RS20930 (FOB45_20930) - 2929282..2931477 (-) 2196 WP_150347953.1 DUF1631 domain-containing protein -
  FOB45_RS20940 (FOB45_20940) - 2932292..2932687 (-) 396 WP_244322630.1 pilin -
  FOB45_RS28650 - 2932691..2932801 (-) 111 Protein_2740 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FOB45_RS20945 (FOB45_20945) pilB 2933021..2934706 (+) 1686 WP_150347955.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOB45_RS20950 (FOB45_20950) pilC 2934838..2936055 (+) 1218 WP_150347956.1 type II secretion system F family protein Machinery gene
  FOB45_RS20955 (FOB45_20955) pilD 2936055..2936924 (+) 870 WP_150347957.1 A24 family peptidase Machinery gene
  FOB45_RS20960 (FOB45_20960) coaE 2936924..2937538 (+) 615 WP_150347958.1 dephospho-CoA kinase -
  FOB45_RS20965 (FOB45_20965) yacG 2937531..2937734 (+) 204 WP_010797204.1 DNA gyrase inhibitor YacG -
  FOB45_RS20970 (FOB45_20970) - 2937825..2938298 (-) 474 WP_010797203.1 hypothetical protein -
  FOB45_RS20975 (FOB45_20975) - 2938502..2938984 (+) 483 WP_125887166.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62142.35 Da        Isoelectric Point: 5.5952

>NTDB_id=387567 FOB45_RS20945 WP_150347955.1 2933021..2934706(+) (pilB) [Pseudomonas luteola strain FDAARGOS_637]
MNDLAPLSGLARQLVLAELLDEKTAQSAVAQSKVSKVPLVTHLVQNRLVKSRAIAELSAEQFGLAFLDLSAVDKESLPKE
IVSEKLMQQHHFLPLAKRGNKLFIGISDPTNQQAINDIQFSTGLTAEPILVEDDKLHNAIDQYLDNGTGGLDLGDIDLDI
EINTEEEKKSEVDADDAPVVRFVNKMLLDAIKRGSSDLHFEPYEKMYRVRFRTDGVMHEMARPPIQLAPRISARLKVMAS
MDISERRKPQDGRVKLKVSKNKSIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEERQKELYMEALSQPQGMILVT
GPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRDLETAEI
AIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPKCKKPHDVPKETLLSEGFPESQIGK
FTLYSPVGCEECNNGYRGRVGIYEVVKITSAMQNLIMEEGNSIEIARKAREEGFNDLRTSGLLKAMQGVTSLEEVNRVTK
D

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=387567 FOB45_RS20945 WP_150347955.1 2933021..2934706(+) (pilB) [Pseudomonas luteola strain FDAARGOS_637]
ATGAATGATTTAGCGCCTCTCTCTGGTCTTGCCCGTCAGCTTGTTTTAGCCGAGCTACTCGATGAAAAAACGGCTCAAAG
CGCAGTTGCGCAGAGCAAGGTCAGTAAAGTCCCGCTGGTGACGCACCTAGTCCAGAATCGCCTCGTTAAGAGTCGGGCAA
TTGCGGAGCTTTCTGCTGAACAATTCGGCTTGGCGTTTTTAGACCTGAGTGCCGTTGATAAGGAGTCCCTGCCCAAGGAG
ATCGTGAGCGAGAAATTGATGCAGCAGCATCACTTCCTGCCGCTCGCCAAACGCGGCAATAAGCTCTTTATCGGTATATC
TGACCCAACCAATCAGCAAGCGATCAATGACATTCAGTTCAGCACAGGGCTGACTGCCGAACCCATACTGGTTGAGGATG
ACAAGCTTCATAATGCCATTGATCAGTACCTGGATAACGGCACCGGTGGGCTGGATCTTGGTGATATTGATCTTGATATC
GAGATCAATACAGAAGAAGAGAAAAAGTCTGAAGTAGACGCGGACGATGCCCCAGTTGTTCGCTTCGTCAATAAAATGCT
GCTCGATGCCATTAAGCGCGGCTCTTCTGATTTGCACTTCGAGCCGTACGAAAAGATGTACCGAGTACGCTTCCGTACCG
ATGGCGTCATGCATGAGATGGCGCGCCCGCCTATTCAACTGGCCCCACGCATTTCGGCTCGCCTGAAGGTCATGGCGAGC
ATGGATATCTCCGAACGCCGCAAGCCGCAAGACGGTCGGGTAAAACTCAAGGTTTCCAAAAACAAGTCCATCGATTTCCG
TGTGAACACGCTTCCGACCCTTTGGGGCGAGAAGATCGTGATGCGAATTCTCGACCCCTCTAGTGCTCAGATGGGTATTG
ATGCATTGGGCTATGAAGAGCGCCAGAAAGAGCTATATATGGAAGCACTTAGCCAACCACAGGGCATGATTCTGGTAACC
GGCCCTACCGGCTCCGGCAAGACCGTATCGCTATACACGGGCTTGAATATCCTAAATACGGTCGACATCAATATCTCGAC
GGCAGAAGACCCGGTAGAGATCAACCTGGAGGGTATTAACCAGGTTAACGTCAATCCGCGTCAAGGAATGGACTTTGCTC
AGGCGCTTAGGGCGTTTCTCCGTCAAGATCCTGACGTGATCATGGTGGGCGAGATCCGCGACCTTGAAACGGCAGAGATT
GCAATCAAGGCTGCACAAACGGGCCACATGGTTATGTCGACGCTGCATACCAACAGCGCGGCAGAAACACTGACGCGCCT
GCGGAATATGGGCGTGCCTGCTTTTAACATCGCCACATCAGTCAACCTAATCATTGCTCAGCGGCTGGCACGCAAGCTGT
GCCCTAAATGCAAAAAGCCGCATGATGTGCCAAAAGAAACCTTGCTGAGTGAAGGCTTCCCTGAAAGCCAGATCGGCAAG
TTTACGCTGTACTCCCCTGTGGGTTGCGAGGAGTGCAACAACGGCTACCGTGGCCGTGTGGGCATCTATGAAGTCGTGAA
GATCACATCTGCCATGCAGAACCTGATCATGGAAGAAGGCAACTCGATCGAGATTGCCCGTAAGGCGCGTGAAGAAGGCT
TTAATGACCTACGGACATCTGGCCTGCTCAAGGCCATGCAAGGTGTAACCAGCCTGGAAGAAGTAAACCGCGTTACCAAA
GACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(185-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.719

100

0.588
  pilB Acinetobacter baylyi ADP1

58.467

100

0.585
  pilB Legionella pneumophila strain ERS1305867

53.439

100

0.54
  pilB Vibrio cholerae strain A1552

50.089

100

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

50

99.109

0.496
  pilB Vibrio campbellii strain DS40M4

48.845

100

0.49
  pilF Neisseria gonorrhoeae MS11

49.013

99.287

0.487
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.662

95.9

0.371
  pilF Thermus thermophilus HB27

37.143

99.822

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

40.039

92.157

0.369

Multiple sequence alignment