Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOB75_RS15925 Genome accession   NZ_CP044062
Coordinates   3122921..3124606 (-) Length   561 a.a.
NCBI ID   WP_150334549.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FDAARGOS_667     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3117921..3129606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB75_RS15895 (FOB75_15895) rplS 3118445..3118798 (+) 354 WP_005462554.1 50S ribosomal protein L19 -
  FOB75_RS15900 (FOB75_15900) yacG 3119093..3119287 (-) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  FOB75_RS15905 (FOB75_15905) zapD 3119356..3120096 (-) 741 WP_023624252.1 cell division protein ZapD -
  FOB75_RS15910 (FOB75_15910) coaE 3120124..3120738 (-) 615 WP_021450019.1 dephospho-CoA kinase -
  FOB75_RS15915 (FOB75_15915) pilD 3120739..3121608 (-) 870 WP_150334548.1 A24 family peptidase Machinery gene
  FOB75_RS15920 (FOB75_15920) pilC 3121673..3122896 (-) 1224 WP_005462561.1 type II secretion system F family protein Machinery gene
  FOB75_RS15925 (FOB75_15925) pilB 3122921..3124606 (-) 1686 WP_150334549.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOB75_RS15930 (FOB75_15930) - 3124611..3125036 (-) 426 WP_025533029.1 type IV pilin protein -
  FOB75_RS15935 (FOB75_15935) nadC 3125299..3126186 (-) 888 WP_025523107.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FOB75_RS15940 (FOB75_15940) ampD 3126279..3126830 (+) 552 WP_025536234.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FOB75_RS15945 (FOB75_15945) pdhR 3127236..3128003 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62417.27 Da        Isoelectric Point: 5.6724

>NTDB_id=387315 FOB75_RS15925 WP_150334549.1 3122921..3124606(-) (pilB) [Vibrio parahaemolyticus strain FDAARGOS_667]
MQTNLSTVLRQKGLLTFSQEESLIEQVKASGISMPEALLSSGLFTSSELAEHLRSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLADFRELSTAIRRLYGRSLSHEKSGLKEINQEELAS
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPNHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETIIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCKQPQEHTVQLQHLGIQTTDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDESLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=387315 FOB75_RS15925 WP_150334549.1 3122921..3124606(-) (pilB) [Vibrio parahaemolyticus strain FDAARGOS_667]
GTGCAAACTAACCTATCAACAGTTCTTCGCCAAAAAGGGTTACTGACATTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCAGGGTTATTCACGTCAAGCGAACTCGCTGAGCACTTAA
GATCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTACGCTTCGCTTTGCCAACAGCTCGGCCTACGTGAA
TTAATCACACGACATAATGCACTCCCACTCCACCGTACTCCTTCAACTTTATTATTAGCGGTTGCCGACCCCACGAATCA
ACAAGCTGAAGATGATTTCCGCTTTGCCACTGGTTTACAGGTTGAGTTGGTGTTGGCGGATTTTCGCGAACTCAGTACAG
CAATTCGACGCTTATATGGTCGTTCATTGAGTCATGAAAAGTCTGGGTTAAAAGAGATCAACCAAGAAGAACTAGCGAGT
CTGGTGGATGTCGGTGCAGATGAAATCGACAATATCGAAGACTTGAGCCAAGACGAATCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCTGTACGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGAGTTCGTC
TACGTTGCGATGGCATTTTGATCGAAACTCAGCAACCGCCAAATCACTTGAGTCGTCGCTTATCAGCTCGTATAAAAATT
CTCTCTAAACTTGATATTGCCGAGCGACGTTTACCACAAGATGGGCGAATTAAACTCAAACTGAATCAAGACACCGCGAT
TGATATGCGGGTATCCACTCTACCTACCTTGTTTGGAGAGAAGATCGTACTACGACTGCTCGATAGCAGCTCCGCATCAC
TGGATATCGACAAGCTTGGTTATAGTGAGCAACAAAAACAGCTGTATTTAGAGGCTCTGCGTCGTCCACAAGGTATGATT
CTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCGTTGTACACTGGGCTAAATATTCTCAATAAACCAGAGATCAA
CATCTCCACAGCGGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAACCTAAGATTGGTTTTG
GCTTTGCAGAAGCACTACGTTCTTTTCTGCGTCAAGATCCCGATGTGGTCATGGTGGGTGAAATTCGAGACCTAGATACT
GCGGAAATCGCCATTAAGGCGTCCCAAACCGGTCACTTAGTGCTCTCTACTTTGCATACCAACTCTGCAGCTGAAACCAT
CATTCGCTTATCAAACATGGGCGTGGAAAGTTTTAACCTCGCCTCTTCACTTAGCTTGATTATTGCCCAACGCCTAGCTC
GCAAACTTTGTCCATATTGTAAACAACCGCAAGAGCATACCGTTCAACTTCAGCACCTCGGCATCCAAACAACTGACAAC
ATCTTTAAAGCCAATCCAGATGGTTGCAACGAATGTACCCATGGTTATTCTGGCCGAACGGGTATCTATGAAGTCATGCG
CTTTGATGAATCTCTATCCGAAGCTCTTATTAAAGGAGCCTCAGTACATGAACTGGAAAAGCTTGCCATTGCGAATGGCA
TGAGCACCTTGCAAATGTCCGGAATAGAAAAACTCAAGCAAGGCATCACAAGCTTTAGTGAGTTACAACGCGTGCTCTAT
TTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

98.574

100

0.986
  pilB Vibrio campbellii strain DS40M4

90.196

100

0.902
  pilB Vibrio cholerae strain A1552

73.31

100

0.734
  pilB Acinetobacter baumannii D1279779

51.521

93.761

0.483
  pilB Legionella pneumophila strain ERS1305867

48.983

96.435

0.472
  pilB Acinetobacter baylyi ADP1

50.677

92.157

0.467
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

37.437

100

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.861

100

0.373
  pilF Thermus thermophilus HB27

36.396

100

0.367

Multiple sequence alignment