Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOB40_RS12755 Genome accession   NZ_CP044060
Coordinates   2815142..2816848 (+) Length   568 a.a.
NCBI ID   WP_150389833.1    Uniprot ID   -
Organism   Aeromonas veronii strain FDAARGOS_632     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2810142..2821848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB40_RS12725 (FOB40_12725) pdhR 2811264..2812028 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FOB40_RS12730 (FOB40_12730) ampD 2812307..2812882 (-) 576 WP_150389828.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FOB40_RS12735 (FOB40_12735) - 2813013..2813483 (+) 471 WP_150389829.1 TIGR02281 family clan AA aspartic protease -
  FOB40_RS12740 (FOB40_12740) nadC 2813487..2814362 (+) 876 WP_150389831.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FOB40_RS12750 (FOB40_12750) - 2814678..2815142 (+) 465 WP_407643249.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FOB40_RS12755 (FOB40_12755) pilB 2815142..2816848 (+) 1707 WP_150389833.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  FOB40_RS12760 (FOB40_12760) pilC 2816978..2818219 (+) 1242 WP_150389834.1 type II secretion system F family protein Machinery gene
  FOB40_RS12765 (FOB40_12765) pilD 2818281..2819153 (+) 873 WP_150389836.1 A24 family peptidase Machinery gene
  FOB40_RS12770 (FOB40_12770) coaE 2819167..2819781 (+) 615 WP_150389838.1 dephospho-CoA kinase -
  FOB40_RS12775 (FOB40_12775) zapD 2819819..2820541 (+) 723 WP_005355104.1 cell division protein ZapD -
  FOB40_RS12780 (FOB40_12780) yacG 2820551..2820745 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  FOB40_RS12785 (FOB40_12785) mutT 2820823..2821227 (-) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62577.93 Da        Isoelectric Point: 5.3215

>NTDB_id=387260 FOB40_RS12755 WP_150389833.1 2815142..2816848(+) (pilB) [Aeromonas veronii strain FDAARGOS_632]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEEIDESEI
SDLEVSDENSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=387260 FOB40_RS12755 WP_150389833.1 2815142..2816848(+) (pilB) [Aeromonas veronii strain FDAARGOS_632]
ATGACTTCTAGTCCTAACAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCTCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGTTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTACATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTAGAGGAAAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAAGAGATCGACGAATCCGAGATT
TCGGATCTCGAGGTCTCCGACGAAAACAGCCGGCTGGACGAGAGCGTCAATACCACCGATGACGATGCCCCTATCGTCAA
GTACATCAACAAAATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGACGGCATTCTGCATGAAATTGCCACACCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCGCGGCTCGATATAGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTCGACGACCGGCAGAAGGCACAGTACCTGCGCGCCCTCTCCAAGCCACAA
GGAATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCCACCGCAGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATACGGGAT
CTAGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCTTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTGTGCGACCACTGCAAGGCACCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAACTGGCGGCCGGGCTTAGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGCGCCAACTCCCTGCAAATCGCCG
CTATCGCCCAGAAGGAAGGGATGCGAACCCTGCGTACCTCAGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGCCTGGCA
GAGGTCAACCGGGTTACCACCAATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.965

100

0.562
  pilB Acinetobacter baumannii D1279779

56.085

99.824

0.56
  pilB Legionella pneumophila strain ERS1305867

56.722

95.599

0.542
  pilB Vibrio cholerae strain A1552

54.937

98.063

0.539
  pilB Vibrio parahaemolyticus RIMD 2210633

51.773

99.296

0.514
  pilB Vibrio campbellii strain DS40M4

51.51

99.12

0.511
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilF Thermus thermophilus HB27

40.53

92.958

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

38.336

97.359

0.373

Multiple sequence alignment