Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FJD32_RS20625 Genome accession   NZ_CP043902
Coordinates   4615068..4616777 (+) Length   569 a.a.
NCBI ID   WP_140907784.1    Uniprot ID   -
Organism   Shewanella sp. LC6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4610068..4621777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJD32_RS20605 (FJD32_020910) nadC 4610361..4611242 (+) 882 WP_140907504.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FJD32_RS20610 (FJD32_020915) - 4611773..4612672 (+) 900 WP_088585327.1 IS982 family transposase -
  FJD32_RS25315 (FJD32_020920) - 4612863..4613276 (+) 414 WP_088586230.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FJD32_RS20620 - 4613324..4614250 (-) 927 WP_181159898.1 O-antigen ligase family protein -
  FJD32_RS20625 (FJD32_020930) pilB 4615068..4616777 (+) 1710 WP_140907784.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FJD32_RS20630 (FJD32_020935) pilC 4616884..4618155 (+) 1272 WP_140907785.1 type II secretion system F family protein Machinery gene
  FJD32_RS20635 (FJD32_020940) pilD 4618309..4619235 (+) 927 WP_140907786.1 A24 family peptidase Machinery gene
  FJD32_RS20640 (FJD32_020945) coaE 4619373..4619990 (+) 618 WP_047534370.1 dephospho-CoA kinase -
  FJD32_RS20645 (FJD32_020950) zapD 4619983..4620717 (+) 735 WP_037413632.1 cell division protein ZapD -
  FJD32_RS20650 (FJD32_020955) yacG 4620820..4621029 (+) 210 WP_037413634.1 DNA gyrase inhibitor YacG -
  FJD32_RS20655 (FJD32_020960) mutT 4621116..4621511 (+) 396 WP_140907787.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62698.12 Da        Isoelectric Point: 6.0579

>NTDB_id=386416 FJD32_RS20625 WP_140907784.1 4615068..4616777(+) (pilB) [Shewanella sp. LC6]
MPSAGLHLGLSTLFIRKGLLNEEQMASAITKSRQNKQTLVTTLVQTKLVSARSIAELCYEEYGTPLLDLAEFDVSAIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEEFQFSAGLHAEAILVEEDKLAKALEKVLEEDITGLDLSGMDEAA
LAGIEITDTDKRNEDNAGESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQPEQIPEHELQRLGF
SDAQIAQGFITYKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAAQAKAQGMRDLRQSGLLKVLQGVTSI
AEVNRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=386416 FJD32_RS20625 WP_140907784.1 4615068..4616777(+) (pilB) [Shewanella sp. LC6]
ATGCCATCCGCTGGCCTTCACCTGGGATTATCCACCCTTTTCATCCGTAAAGGTCTGCTTAATGAAGAGCAGATGGCGAG
TGCCATCACCAAATCCCGCCAAAATAAACAAACCCTAGTCACCACCTTAGTGCAAACTAAGCTCGTGTCGGCTCGCAGTA
TTGCCGAGCTTTGCTATGAGGAATATGGCACCCCGCTGTTGGATTTAGCCGAATTTGATGTTAGCGCCATCCCTGAAGAG
TTTCTTAATAAAAAACTGATTGAAAAGCACCGCTGCTTACCGCTATTTAAGCGCGGCAATCGCCTCTATATTGCCACCTC
AGATCCTACCAATATTGCCGCCCTAGAAGAGTTTCAATTTAGTGCAGGCCTACATGCCGAAGCCATTTTGGTGGAAGAAG
ATAAGCTCGCTAAGGCCCTTGAAAAGGTACTCGAAGAAGATATTACTGGCCTTGATTTAAGCGGAATGGATGAAGCAGCG
CTTGCTGGTATCGAAATTACCGATACCGATAAACGCAATGAAGACAATGCTGGTGAATCCAGTGACGATGCCCCTATCGT
CATTTATATCAATAAGATACTAACCGATGCTATTCGTAAGGGCGCTTCAGACTTACATTTTGAGCCCTATGAAAAACGCT
ATCGTATCCGTTTTCGTATCGATGGTATTTTGCATGAAGTCTCCGAGCCGCCAATCAGCCTAGCCGGACGACTCTCAGCG
CGCTTAAAGGTAATGTCAAAGCTGGATATTGCCGAGCGTCGCGTCCCCCAGGATGGCCGGATTAAAATGAAGCTATCCCG
CACTAAGTCTATCGACTTTCGGGTAAGTACCCTGCCGACCCTGTGGGGCGAAAAAATTGTAATGCGGATATTGGATTCTT
CTTCCGCACAGCTCGGTATCGAAAAACTCGGCTACGAACCCGATCAAGAAAAGCTCTACCTTGAGATGCTAGCCAAACCC
CAAGGCATGATATTAGTGACCGGCCCAACAGGTTCGGGTAAAACCGTTTCGCTCTATACAGGCTTAAATATCCTCAATAC
CGCCGAGCGTAATATTTCCACCGCCGAAGATCCGGTGGAAATTAACCTTGAGGGGGTAAACCAAGTCCATATTAATTTAA
AAGCAGGTTTAACCTTCGCCTCAGCACTACGTTCGTTTTTGCGCCAAGACCCCGATGTGGTGATGGTCGGGGAAATCCGT
GACCTTGAAACCGCCGAAATTGCCATTAAAGCCGCACAAACAGGCCATTTAGTATTATCAACACTGCATACTAACTCGGC
GGCAGAAACGCTCACCCGTTTAATTAATATGGGCGTACCCGGCTATAATATTGCCAGCTCAGTCAATTTAATTATTGCTC
AGCGACTTGCACGGCGACTTTGCCCAGAATGCAAACAACCCGAACAAATACCCGAACATGAGCTACAACGTTTAGGCTTT
AGTGATGCGCAAATCGCCCAAGGGTTTATCACCTATAAACCTGTGGGCTGCGAGCATTGCTCCGGAGGCTATAAAGGACG
GGTAGGCATTTACGAAGTGATGAAGATGTCAGATGAAATTGCCCGTACTATTATGGAAGGGGGTAATTCATTACAAATTG
CTGCCCAAGCCAAGGCCCAAGGCATGCGCGATCTGCGTCAATCAGGGCTACTAAAAGTGCTCCAAGGTGTAACCAGTATT
GCCGAAGTGAATCGGGTAACCAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.893

98.594

0.591
  pilB Acinetobacter baumannii D1279779

59.266

95.782

0.568
  pilB Legionella pneumophila strain ERS1305867

56.94

98.77

0.562
  pilB Vibrio parahaemolyticus RIMD 2210633

52.014

100

0.522
  pilB Vibrio cholerae strain A1552

53.153

97.54

0.518
  pilB Vibrio campbellii strain DS40M4

51.237

99.473

0.51
  pilF Neisseria gonorrhoeae MS11

49.117

99.473

0.489
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.464

98.418

0.388
  pilF Thermus thermophilus HB27

38.393

98.418

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

41

87.873

0.36

Multiple sequence alignment