Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F0U83_RS04590 Genome accession   NZ_CP043869
Coordinates   977678..979390 (-) Length   570 a.a.
NCBI ID   WP_138988747.1    Uniprot ID   A0A5P1R9S8
Organism   Neptunomonas concharum strain JCM17730     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 972678..984390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0U83_RS04565 (F0U83_04565) - 973802..974743 (+) 942 WP_138988742.1 Nudix family hydrolase -
  F0U83_RS04570 (F0U83_04570) - 974724..974942 (-) 219 WP_138988743.1 DNA gyrase inhibitor YacG -
  F0U83_RS04575 (F0U83_04575) coaE 974939..975553 (-) 615 WP_138988744.1 dephospho-CoA kinase -
  F0U83_RS04580 (F0U83_04580) pilD 975562..976428 (-) 867 WP_138988745.1 prepilin peptidase Machinery gene
  F0U83_RS04585 (F0U83_04585) pilC 976436..977659 (-) 1224 WP_138988746.1 type II secretion system F family protein Machinery gene
  F0U83_RS04590 (F0U83_04590) pilB 977678..979390 (-) 1713 WP_138988747.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F0U83_RS04595 (F0U83_04595) comP 979630..980055 (+) 426 WP_138988748.1 pilin Machinery gene
  F0U83_RS17215 (F0U83_04600) comP 980127..980564 (+) 438 WP_138988749.1 pilin Machinery gene
  F0U83_RS04605 (F0U83_04605) - 980580..980924 (+) 345 WP_138988750.1 hypothetical protein -
  F0U83_RS04610 (F0U83_04610) nadC 980970..981809 (-) 840 WP_138988751.1 carboxylating nicotinate-nucleotide diphosphorylase -
  F0U83_RS04615 (F0U83_04615) ampD 981899..982456 (+) 558 WP_246077868.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F0U83_RS04620 (F0U83_04620) - 982525..983574 (+) 1050 WP_138988752.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62938.20 Da        Isoelectric Point: 5.9565

>NTDB_id=386306 F0U83_RS04590 WP_138988747.1 977678..979390(-) (pilB) [Neptunomonas concharum strain JCM17730]
MQPQQPLSGLARRLVKDGVFSAEVAREAIEKARASEKPFITHIIDERLVSAHRAASAASDEFGLPLFDLAAMNIESVPQG
LVSDKLINKHHAIPLIKRDSRIYVALADPTNIQALDEFKFHTGVTTEGVIVEEDKLRKFIESFLDNQGSALDELDDADLD
SLEVDDGSVPEEEITSSEDAAKDAPIVRFVNKILLDCIKKGASDIHFEPYEKTYRIRSRIDGILQEIAKPPHNLSSRLSA
RLKVMSQMDISEKRMPQDGRIKMKISKTRAIDFRVNTLPTLWGEKIVLRILDPSSAKMGVDALGFNPVQKELYMGTLHKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLTFAEALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSSPETLTRLRNMGVPSFNIATSVSLIIAQRLARRLCESCKKPVSIPEAALIEEGF
GPEQLKNLHLHEANPDGCSKCNRGYKGRVGIYEMLPMTPEIAEVIMNNGTSLDIIRVAKQQGFRTLRDTGLDKVAEGLTS
LDEMNRVIKT

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=386306 F0U83_RS04590 WP_138988747.1 977678..979390(-) (pilB) [Neptunomonas concharum strain JCM17730]
GTGCAACCACAGCAACCCCTTAGCGGTTTAGCACGACGCTTAGTAAAAGACGGTGTTTTTTCGGCAGAGGTTGCCCGCGA
GGCGATCGAAAAAGCCAGGGCTTCGGAAAAGCCTTTTATCACCCATATAATTGATGAACGCTTAGTCAGTGCCCATCGAG
CTGCCAGTGCTGCATCCGATGAGTTCGGCTTGCCCCTGTTTGATTTAGCGGCCATGAATATCGAATCGGTACCTCAAGGA
TTAGTCTCTGACAAACTGATTAACAAGCATCACGCCATCCCTCTAATCAAACGGGATAGCCGTATCTATGTAGCGCTGGC
AGACCCCACCAATATTCAGGCATTGGATGAGTTTAAGTTCCACACTGGCGTCACCACCGAAGGGGTCATTGTTGAGGAGG
ATAAGCTTCGTAAATTTATTGAGTCCTTCTTAGATAACCAAGGCTCGGCACTGGATGAGCTGGATGATGCCGACCTAGAT
TCTCTCGAAGTCGATGACGGCAGCGTACCTGAAGAAGAGATCACAAGCAGTGAAGATGCCGCCAAAGATGCGCCTATTGT
CCGTTTTGTAAATAAAATTTTATTAGATTGTATTAAAAAAGGCGCTTCGGATATTCATTTTGAGCCTTATGAGAAAACCT
ACCGTATACGAAGCCGTATCGATGGTATTTTGCAAGAGATCGCCAAGCCGCCTCATAATCTGTCTAGCAGACTTTCGGCA
CGGCTCAAGGTGATGTCACAAATGGATATCTCTGAGAAAAGGATGCCTCAGGATGGCCGTATCAAGATGAAAATTTCTAA
AACACGAGCCATCGACTTCCGTGTTAATACCTTGCCCACCCTTTGGGGTGAAAAGATCGTACTACGTATTCTCGACCCAT
CCAGTGCTAAGATGGGGGTCGACGCACTGGGCTTTAACCCAGTACAAAAAGAGCTGTATATGGGCACGCTACACAAGCCC
CAAGGGATGATTTTGGTGACCGGGCCAACCGGTAGTGGTAAAACCGTCTCGCTTTATACGGGCCTTAATATCCTAAATAC
CGAAGAGCGTAATATCTCCACCGCAGAAGACCCAGTGGAGATCAACTTAGAAGGTATTAACCAAGTCCACGTTAATGCTA
AAGTAGGTTTAACCTTTGCTGAGGCGCTTCGTTCATTCCTGCGTCAGGATCCGGATGTTGTAATGGTAGGTGAGATCAGG
GATTTGGAAACGGCCGAAATTGCCATTAAAGCCGCACAAACCGGCCATATGGTGTTATCAACACTGCACACCAACAGTTC
ACCCGAAACCCTGACCCGTTTACGTAATATGGGTGTGCCTTCGTTTAATATTGCCACATCCGTAAGCTTAATTATTGCGC
AACGTCTTGCTAGGCGCTTGTGCGAAAGCTGCAAAAAGCCGGTCAGTATCCCAGAAGCGGCCTTGATTGAAGAGGGTTTC
GGCCCTGAGCAGCTAAAAAACCTGCACCTGCATGAGGCTAACCCTGATGGCTGTAGCAAATGTAACCGTGGCTATAAGGG
CCGAGTGGGTATCTATGAGATGCTACCCATGACACCCGAAATTGCCGAAGTTATTATGAACAATGGCACATCGCTGGATA
TTATTCGCGTCGCCAAACAGCAGGGCTTTAGAACCCTGCGGGATACCGGCTTAGATAAAGTCGCCGAAGGGCTTACCAGC
CTTGATGAAATGAATCGTGTAATTAAAACCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P1R9S8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.333

98.947

0.577
  pilB Acinetobacter baylyi ADP1

57.829

98.596

0.57
  pilB Legionella pneumophila strain ERS1305867

54.674

99.474

0.544
  pilB Vibrio cholerae strain A1552

51.053

100

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

51.237

99.298

0.509
  pilB Vibrio campbellii strain DS40M4

50.265

99.474

0.5
  pilF Neisseria gonorrhoeae MS11

48.75

98.246

0.479

Multiple sequence alignment