Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F1538_RS09070 Genome accession   NZ_CP043771
Coordinates   1822233..1822682 (-) Length   149 a.a.
NCBI ID   WP_006996184.1    Uniprot ID   A0AAV2U2D0
Organism   Haemophilus influenzae biotype aegyptius strain HE15/F3028     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1817233..1827682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1538_RS09050 (F1538_09060) rho 1817622..1818884 (+) 1263 WP_005666690.1 transcription termination factor Rho -
  F1538_RS09055 (F1538_09065) pilD 1818939..1819631 (-) 693 WP_013525531.1 A24 family peptidase Machinery gene
  F1538_RS09060 (F1538_09070) pilC 1819628..1820845 (-) 1218 WP_013525532.1 type II secretion system F family protein Machinery gene
  F1538_RS09065 (F1538_09075) pilB 1820842..1822236 (-) 1395 WP_013525533.1 GspE/PulE family protein Machinery gene
  F1538_RS09070 (F1538_09080) pilA 1822233..1822682 (-) 450 WP_006996184.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  F1538_RS09075 (F1538_09085) ampD 1822797..1823357 (+) 561 WP_006996183.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F1538_RS09080 (F1538_09090) corC 1823980..1824879 (+) 900 WP_013525534.1 CNNM family magnesium/cobalt transport protein CorC -
  F1538_RS09085 (F1538_09095) lnt 1824902..1826431 (+) 1530 WP_013525535.1 apolipoprotein N-acyltransferase -
  F1538_RS09090 (F1538_09100) rsmE 1826481..1827218 (+) 738 WP_006996180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15542.86 Da        Isoelectric Point: 8.0922

>NTDB_id=385826 F1538_RS09070 WP_006996184.1 1822233..1822682(-) (pilA) [Haemophilus influenzae biotype aegyptius strain HE15/F3028]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITAAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=385826 F1538_RS09070 WP_006996184.1 1822233..1822682(-) (pilA) [Haemophilus influenzae biotype aegyptius strain HE15/F3028]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCGCAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTTACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

99.329

100

0.993
  pilA Haemophilus influenzae Rd KW20

91.275

100

0.913
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

42.177

98.658

0.416
  pilA2 Legionella pneumophila strain ERS1305867

42.177

98.658

0.416
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376

Multiple sequence alignment