Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F1C79_RS30070 Genome accession   NZ_CP043626
Coordinates   6457170..6458873 (-) Length   567 a.a.
NCBI ID   WP_151189417.1    Uniprot ID   A0A9X7N5V5
Organism   Pseudomonas denitrificans (nom. rej.) strain BG1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6452170..6463873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1C79_RS30035 (F1C79_30050) - 6452678..6453142 (+) 465 WP_231708957.1 FAD/FMN-containing dehydrogenase -
  F1C79_RS30040 (F1C79_30055) - 6453139..6453828 (+) 690 WP_151189415.1 energy-coupling factor ABC transporter permease -
  F1C79_RS30045 (F1C79_30060) - 6453936..6454151 (+) 216 WP_081517369.1 hypothetical protein -
  F1C79_RS30050 (F1C79_30065) yacG 6454201..6454404 (-) 204 WP_017516736.1 DNA gyrase inhibitor YacG -
  F1C79_RS30055 (F1C79_30070) coaE 6454401..6455009 (-) 609 WP_081517370.1 dephospho-CoA kinase -
  F1C79_RS30060 (F1C79_30075) - 6455074..6455945 (-) 872 Protein_5982 A24 family peptidase -
  F1C79_RS30065 (F1C79_30080) pilC 6455949..6457166 (-) 1218 WP_151189416.1 type II secretion system F family protein Machinery gene
  F1C79_RS30070 (F1C79_30085) pilB 6457170..6458873 (-) 1704 WP_151189417.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F1C79_RS30075 (F1C79_30090) comP 6459102..6459503 (+) 402 WP_151189418.1 pilin Machinery gene
  F1C79_RS30080 (F1C79_30095) - 6460109..6461461 (+) 1353 WP_167523263.1 sulfatase-like hydrolase/transferase -
  F1C79_RS30090 (F1C79_30105) galE 6461895..6462911 (-) 1017 WP_151189420.1 UDP-glucose 4-epimerase GalE -
  F1C79_RS30095 (F1C79_30110) nadC 6463020..6463868 (-) 849 WP_151189421.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62529.70 Da        Isoelectric Point: 5.7235

>NTDB_id=385550 F1C79_RS30070 WP_151189417.1 6457170..6458873(-) (pilB) [Pseudomonas denitrificans (nom. rej.) strain BG1]
MNDQVVLSGLARQLVHSELLDEKSAQQAQLQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGVAYCDLSGIDRDAIPKE
LISEKLIRQHRVIPLWRRGNKLFVGLSDPTNHQAITDVQFSTGLTTEAILVEDDKLGDMIEKIFESASGGLDDLGDVDLE
ALDIESGNAEPKDDVIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRLSAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDASSAQMGIDALGYEEEQKQLYLNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVAAFNLATSTNLIIAQRLARKLCPHCKKEHDVPQEALLHEGFP
ADRIGTFKLYSPVGCDNCKGGYKGRLGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=385550 F1C79_RS30070 WP_151189417.1 6457170..6458873(-) (pilB) [Pseudomonas denitrificans (nom. rej.) strain BG1]
ATGAACGATCAAGTCGTGCTCTCGGGTCTCGCCCGTCAACTGGTGCATTCAGAATTACTGGACGAGAAGAGCGCCCAGCA
GGCGCAACTGCAGGCGCAACGGAACAAGTTGTCGCTGGTCACCTACCTTGTGCAGAACAAGCTGGTGAAGAGCCGCCCGT
TGACGGAGTTGGCTGCAGACCAATTTGGCGTCGCTTACTGCGATTTATCAGGTATCGACCGGGACGCGATTCCGAAGGAG
TTGATCAGCGAGAAGCTGATCCGCCAGCATCGGGTGATCCCGCTCTGGCGCCGAGGCAACAAGCTGTTCGTCGGCTTATC
CGACCCGACCAACCATCAGGCAATTACAGACGTTCAGTTCAGCACCGGGCTGACGACCGAAGCCATCCTGGTCGAGGATG
ACAAGCTTGGCGATATGATCGAGAAGATCTTCGAGAGCGCCTCTGGTGGGCTCGACGACTTGGGCGACGTGGACCTCGAA
GCCCTCGACATCGAAAGCGGTAATGCAGAACCCAAAGATGACGTCATTGGCGGCGATGCCGATGATGCGCCAGTGGTCCG
CTTCGTCAACAAGATGTTGCTGGACGCTATTAAGGGGGGATCATCCGACCTGCACTTCGAGCCCTATGAAAAGATCTATC
GTGTGCGCTTCCGTACCGACGGCATGCTCCACGAGGTTGCCAAACCACCTATCCAGCTCGCCAGCCGTCTCTCAGCGCGC
CTGAAGGTCATGGCCGGCCTGGATATCTCCGAGCGCCGCAAGCCACAGGACGGTCGGATCAAGATGCGCGTGTCCAAGAC
CAAATCCATCGACTTCCGCGTCAACACCCTTCCGACACTGTGGGGCGAGAAGATCGTGATGCGAATCCTGGACGCATCCA
GCGCACAGATGGGGATCGATGCACTGGGCTATGAGGAAGAACAGAAGCAACTCTACCTCAACGCCTTGAAGCAACCACAA
GGCATGATCCTAGTGACCGGTCCCACTGGCTCCGGCAAAACGGTGTCGCTCTATACCGGCCTGAACATCCTCAACACCCC
GGACATCAATATTTCCACGGCGGAAGACCCGGTAGAGATCAACCTGGAAGGGATCAACCAGGTCAACGTCAACCCCAAGC
AGGGCATGGACTTCGCCCAGGCGCTGCGCGCGTTCCTGCGGCAGGACCCTGACGTGATCATGGTCGGTGAGATCCGTGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCACAGACTGGCCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
AGAAACCCTGACACGCCTGCTGAACATGGGCGTTGCCGCGTTCAACCTGGCGACGTCCACCAACCTCATCATCGCCCAGC
GCCTGGCACGTAAGCTCTGCCCCCATTGCAAGAAAGAGCATGACGTCCCGCAGGAAGCGCTCCTGCATGAAGGTTTCCCG
GCGGACCGGATCGGGACGTTCAAGCTGTACTCGCCCGTCGGGTGTGACAATTGCAAGGGCGGATACAAGGGACGCTTGGG
TATTTATGAAGTGGTTAAAATCACGCCAGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATTCAGATCGCCGAAC
AGGCCCGCAAAGAAGGCTTCAACGATCTGCGCACTTCGGGACTGCTGAAGGCCATGCAAGGCATCACCAGTCTCGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.624

99.471

0.573
  pilB Acinetobacter baumannii D1279779

57.624

99.471

0.573
  pilB Legionella pneumophila strain ERS1305867

54.321

100

0.543
  pilB Vibrio cholerae strain A1552

51.064

99.471

0.508
  pilF Neisseria gonorrhoeae MS11

49.912

100

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

52.126

95.414

0.497
  pilB Vibrio campbellii strain DS40M4

51.206

95.062

0.487
  pilF Thermus thermophilus HB27

37.658

97.884

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

39.808

91.711

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.298

0.365

Multiple sequence alignment