Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   F0Q09_RS06340 Genome accession   NZ_CP043576
Coordinates   1398683..1399945 (-) Length   420 a.a.
NCBI ID   WP_080756735.1    Uniprot ID   -
Organism   Pseudomonas putida strain T25-27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1393683..1404945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0Q09_RS06325 - 1395349..1396950 (-) 1602 WP_003249151.1 SPOR domain-containing protein -
  F0Q09_RS06330 aroB 1396966..1398063 (-) 1098 WP_003249149.1 3-dehydroquinate synthase -
  F0Q09_RS06335 aroK 1398160..1398678 (-) 519 WP_003249147.1 shikimate kinase AroK -
  F0Q09_RS06340 pilQ 1398683..1399945 (-) 1263 WP_080756735.1 type IV pilus secretin PilQ Machinery gene
  F0Q09_RS06345 - 1399869..1400870 (-) 1002 WP_047603626.1 pilus assembly protein PilP -
  F0Q09_RS06350 - 1400882..1401418 (-) 537 WP_004575490.1 PilN domain-containing protein -
  F0Q09_RS06355 pilM 1401415..1402296 (-) 882 WP_047603628.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45718.41 Da        Isoelectric Point: 4.9839

>NTDB_id=385269 F0Q09_RS06340 WP_080756735.1 1398683..1399945(-) (pilQ) [Pseudomonas putida strain T25-27]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQVLDAQLQPLRRELLTIHHAKAADLAQLLLASLEGGSISTGSLSVDERT
NTLVVYLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=385269 F0Q09_RS06340 WP_080756735.1 1398683..1399945(-) (pilQ) [Pseudomonas putida strain T25-27]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTCG
CCAGTGATGCGGTACAAGGCAGCGTCACTTTGCGCTTGCAGGACGTGCCGTGGGATCAGGCGCTCGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGCCAAGTGCTGGATGCGCAGCTGCAGCCATTGCGTCGGGAGCTGCTGACCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCTGGCAAGTCTGGAGGGTGGCAGCATCTCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGTTGGTGGCGCAGTTGGACGTGCCGGTGCG
TCAGGTAGCGATTGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAGCGAGCCGGCAGCAGTATCGGCCTG
GGTTTGTTGCGCGGTGACGTGTTGCTGGATCTGGAACTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACCAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACTCCGGACAACAAGGTG
ATCATGACCGTCCGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(126-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.617
  comQ Acinetobacter baylyi ADP1

42.889

100

0.46
  pilQ Acinetobacter baumannii strain A118

40.781

100

0.448
  pilQ Acinetobacter baumannii D1279779

40.781

100

0.448
  pilQ Legionella pneumophila strain ERS1305867

43.269

99.048

0.429
  pilQ Legionella pneumophila strain Lp02

43.269

99.048

0.429
  pilQ Vibrio campbellii strain DS40M4

41.014

100

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.169

98.81

0.417
  pilQ Vibrio cholerae strain A1552

42.169

98.81

0.417

Multiple sequence alignment