Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   F0Q02_RS03930 Genome accession   NZ_CP043573
Coordinates   864587..865804 (-) Length   405 a.a.
NCBI ID   WP_003058543.1    Uniprot ID   B7WUY7
Organism   Comamonas testosteroni strain R5-28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 859587..870804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0Q02_RS03900 - 860400..861335 (+) 936 WP_003058555.1 ATP-binding protein -
  F0Q02_RS03905 - 861348..861818 (+) 471 WP_019043048.1 NUDIX domain-containing protein -
  F0Q02_RS03910 - 861934..862146 (-) 213 WP_003058551.1 DNA gyrase inhibitor YacG -
  F0Q02_RS03915 zapD 862245..863000 (-) 756 WP_003058549.1 cell division protein ZapD -
  F0Q02_RS03920 coaE 863088..863714 (-) 627 WP_003058547.1 dephospho-CoA kinase -
  F0Q02_RS03925 - 863718..864587 (-) 870 WP_003058545.1 prepilin peptidase -
  F0Q02_RS03930 pilC 864587..865804 (-) 1218 WP_003058543.1 type II secretion system F family protein Machinery gene
  F0Q02_RS03935 pilB 865828..867570 (-) 1743 WP_003058542.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F0Q02_RS03945 - 867825..868754 (-) 930 WP_003058538.1 polyprenyl synthetase family protein -
  F0Q02_RS03950 rplU 869097..869408 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  F0Q02_RS03955 rpmA 869425..869682 (+) 258 WP_003058534.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44562.30 Da        Isoelectric Point: 9.4547

>NTDB_id=385227 F0Q02_RS03930 WP_003058543.1 864587..865804(-) (pilC) [Comamonas testosteroni strain R5-28]
MASAASKGIKEYLFEWEGKDRNGKIVRGETRAGGENQIQAMLRRQGVTPSKIKKRRTRGGKKIKPKDIALFTRQLATMMK
AGVPLLQSFDIVGRGNTNPNVTKLLNDIRSDVETGTSLSAAFRKFPLYFDSLYCNLVEAGEAAGILESLLDRLATYMEKT
EAIKSKIKSALMYPTSVMIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMGISEYFVQYWWLIFGVLGGGIYFFM
QAWKRNERVQQFMDRTMLKLPIFGVLIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNYLYKNATDRIQSEVSTGT
SLTVAMANANIFPSMVLQMCAIGEESGAIDHMLGKAADFYESEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=385227 F0Q02_RS03930 WP_003058543.1 864587..865804(-) (pilC) [Comamonas testosteroni strain R5-28]
ATGGCAAGCGCAGCGTCCAAGGGAATCAAGGAATATCTCTTCGAGTGGGAAGGCAAGGACCGCAACGGCAAGATCGTGCG
CGGCGAAACGCGTGCCGGGGGGGAGAACCAGATCCAGGCCATGCTGCGCCGCCAGGGCGTGACTCCGTCCAAGATCAAGA
AGCGCAGGACCCGCGGCGGCAAGAAGATCAAGCCCAAGGACATTGCACTCTTCACGCGCCAACTGGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCTTTCGACATCGTGGGCCGCGGCAATACCAACCCCAATGTCACCAAGCTGCTCAACGA
CATCCGCTCCGATGTGGAGACCGGCACCTCGCTGAGCGCTGCCTTTCGCAAGTTTCCGCTCTATTTCGACAGTCTCTACT
GCAACCTGGTGGAGGCCGGCGAGGCCGCAGGTATTCTGGAATCGCTGCTGGACCGTCTTGCCACCTATATGGAGAAGACG
GAAGCCATCAAGTCCAAGATCAAGTCCGCGCTGATGTACCCCACATCGGTGATGATCGTGGCCTTTGTCGTGGTGACCGT
GATCATGATCTTCGTGATTCCGGCCTTCAAGGAGGTCTTCACCTCCTTCGGCGCCGACCTGCCCGCACCCACGCTGCTGG
TGATGGGCATCAGTGAGTACTTTGTGCAGTACTGGTGGCTGATCTTCGGCGTGCTGGGCGGCGGCATCTATTTCTTCATG
CAGGCCTGGAAGCGCAACGAGCGCGTGCAGCAGTTCATGGACCGGACCATGCTCAAACTGCCCATCTTCGGCGTGCTGAT
CGAGAAATCCTGCGTGGCCCGCTGGACGCGCACGCTGTCCACCATGTTTGCAGCCGGCGTGCCGCTGGTCGAGGCACTGG
ACTCCGTGGGAGGCGCCTCGGGCAACTACCTCTACAAAAACGCCACCGACAGGATTCAGTCGGAGGTCTCCACGGGCACC
AGCCTCACGGTTGCCATGGCCAATGCCAATATCTTCCCTTCCATGGTGCTGCAGATGTGCGCCATCGGCGAGGAATCGGG
CGCCATCGACCATATGCTGGGCAAGGCCGCCGACTTCTATGAAAGCGAAGTGGACGAAATGGTGGCGGGTCTGTCCAGCC
TGATGGAGCCCATCATCATCGTCTTCCTGGGCACCTTGATCGGCGGCATCGTGGTGTCCATGTATCTGCCTATCTTCAAG
CTGGGTCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7WUY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.208

99.753

0.541
  pilG Neisseria gonorrhoeae MS11

51.889

98.025

0.509
  pilG Neisseria meningitidis 44/76-A

51.637

98.025

0.506
  pilC Legionella pneumophila strain ERS1305867

51.01

97.778

0.499
  pilC Acinetobacter baylyi ADP1

50

98.272

0.491
  pilC Acinetobacter baumannii D1279779

48.615

98.025

0.477
  pilC Vibrio cholerae strain A1552

40.554

98.025

0.398
  pilC Vibrio campbellii strain DS40M4

38.308

99.259

0.38
  pilC Thermus thermophilus HB27

36.386

99.753

0.363

Multiple sequence alignment