Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FYK34_RS13930 Genome accession   NZ_CP043473
Coordinates   2943879..2945582 (-) Length   567 a.a.
NCBI ID   WP_149297374.1    Uniprot ID   -
Organism   Chromobacterium paludis strain IIBBL 257-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2938879..2950582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYK34_RS13905 (FYK34_13905) yacG 2940086..2940280 (-) 195 WP_149297365.1 DNA gyrase inhibitor YacG -
  FYK34_RS13910 (FYK34_13910) zapD 2940286..2941044 (-) 759 WP_149297367.1 cell division protein ZapD -
  FYK34_RS13915 (FYK34_13915) coaE 2941081..2941695 (-) 615 WP_149297368.1 dephospho-CoA kinase -
  FYK34_RS13920 (FYK34_13920) pilD 2941680..2942600 (-) 921 WP_149297370.1 A24 family peptidase Machinery gene
  FYK34_RS13925 (FYK34_13925) pilC 2942600..2943817 (-) 1218 WP_149297372.1 type II secretion system F family protein Machinery gene
  FYK34_RS13930 (FYK34_13930) pilF 2943879..2945582 (-) 1704 WP_149297374.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FYK34_RS13935 (FYK34_13935) - 2945777..2946922 (-) 1146 WP_149297376.1 porin -
  FYK34_RS13940 (FYK34_13940) comE 2947495..2947815 (-) 321 WP_149297378.1 helix-hairpin-helix domain-containing protein Machinery gene
  FYK34_RS13945 (FYK34_13945) - 2947867..2948688 (-) 822 WP_149297380.1 inner membrane protein YpjD -
  FYK34_RS13950 (FYK34_13950) ffh 2948802..2950157 (+) 1356 WP_149297382.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62170.84 Da        Isoelectric Point: 5.8497

>NTDB_id=384393 FYK34_RS13930 WP_149297374.1 2943879..2945582(-) (pilF) [Chromobacterium paludis strain IIBBL 257-1]
MEATAGISGLGRALVQHNMLLQQDADAIQKHAAASHISFVEQLILSKKMSAREVAVFASRIFGYPLLDMSALDIEQLPKG
LVDEELIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEIVVVEDDKLSKLVGRYQDSPAAALKELEGEDFG
DLEFADPEAQADAPQVEVDDAPVVKFIHKMLLDGIGVGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRIK
VISKLDISEKRVPQDGRLKLVISKTRAIDFRVSTLPTLYGEKIVMRILDSSSASLDIEQLGFEPEQKQLLLDAIARPYGM
VLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALRAFLRQDPDVIMVGEIRDFE
TADIAIKAAQTGHMVFSTLHTNNAPATLTRLLNMGVAPFNIASSVLLIMAQRLARRLCNHCKKPLDIPQEALLRAGFAQE
ELDGSWHPYGPVGCDECRNTGYKGRVGIYEVMPISDAMTRLIMKNGTAMDISDLALQEGMVNLRHAGLLKVKKGMTSLAE
IEAVTND

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=384393 FYK34_RS13930 WP_149297374.1 2943879..2945582(-) (pilF) [Chromobacterium paludis strain IIBBL 257-1]
ATGGAAGCAACGGCAGGAATTTCCGGCCTCGGACGGGCCTTGGTTCAGCACAATATGTTGCTGCAGCAAGATGCGGACGC
CATTCAAAAACACGCGGCGGCCTCGCATATCAGTTTTGTCGAGCAGTTGATACTGAGCAAGAAAATGAGCGCGCGCGAGG
TGGCGGTATTCGCTTCGCGCATTTTCGGTTATCCCTTGCTGGACATGAGCGCGCTGGATATCGAGCAGCTTCCCAAAGGC
TTGGTGGATGAAGAGCTGATCGCCAGCCGCCGTCTGCTGCCGCTGCTCAAACGCGGCAACAAGTTGTATATCGGCACCTC
CGATCCGACGCAGACCTCTGCCTACCACGCCATTACTTTCAAAACCGGCTTGACAGTGGAGATCGTTGTCGTCGAAGACG
ACAAGTTGTCCAAGCTGGTGGGGCGTTACCAGGACAGCCCGGCCGCCGCCTTGAAAGAGCTCGAAGGCGAGGATTTTGGC
GACTTGGAGTTCGCCGATCCGGAAGCGCAGGCGGACGCCCCGCAAGTGGAGGTGGACGATGCCCCGGTGGTCAAGTTCAT
CCACAAGATGCTGCTGGACGGCATAGGCGTGGGCGCGTCCGACATCCATTTCGAACCCTATGAAAAGTATTACCGCATCC
GCTACCGCGTGGACGGCGTATTGAAGGAGGTGGCGCAGCCTCCCTTGCTGCTGAAGGAAAAAATCGCTTCCCGCATCAAG
GTGATTTCCAAGCTGGACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGCTTGAAGCTGGTGATTTCCAAGACCCGAGC
CATCGATTTCCGGGTCAGCACGCTGCCGACGCTATATGGCGAGAAAATCGTGATGCGGATTCTGGACTCCTCGTCGGCGT
CGCTGGATATCGAACAACTGGGCTTCGAACCAGAGCAAAAACAACTGTTGTTGGACGCCATCGCGCGGCCCTATGGCATG
GTTTTGGTCACCGGGCCGACCGGCAGCGGCAAAACAGTGTCGCTGTATACCTGCCTGAATATTTTGAACAAGCCGGACAC
CAATATCTCCACGGCGGAGGATCCGGTGGAAATCAACCTGCCGGGCATCAATCAAGTCAATGTCAATGAAAAGGCGGGCC
TGACCTTCGCTTCCGCGTTGCGCGCCTTTCTGCGGCAAGATCCGGATGTGATCATGGTGGGCGAAATCCGCGACTTCGAG
ACCGCCGACATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGTTCTCCACCTTGCACACCAATAACGCGCCGGCCAC
GTTGACGCGGCTGTTGAACATGGGCGTCGCGCCGTTCAATATCGCCAGTTCGGTGCTGCTGATCATGGCGCAGCGTCTGG
CGCGCCGCTTGTGCAACCATTGCAAAAAGCCGTTGGATATTCCGCAGGAGGCCCTGCTGCGCGCCGGCTTCGCCCAGGAG
GAGCTGGATGGCAGCTGGCATCCCTACGGGCCGGTGGGATGCGATGAGTGCCGCAATACCGGGTACAAGGGCCGGGTCGG
CATCTACGAGGTCATGCCTATCAGCGACGCGATGACGCGCTTGATCATGAAAAACGGCACCGCGATGGATATCTCCGATC
TGGCCTTGCAGGAGGGCATGGTGAATTTGCGCCATGCGGGCCTGCTCAAGGTGAAGAAGGGCATGACCTCGCTGGCGGAA
ATCGAAGCGGTCACCAACGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.915

99.471

0.566
  pilB Acinetobacter baumannii D1279779

54.281

100

0.559
  pilB Legionella pneumophila strain ERS1305867

54.545

100

0.55
  pilB Acinetobacter baylyi ADP1

54.965

99.471

0.547
  pilB Vibrio cholerae strain A1552

51.079

98.06

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

48.841

98.942

0.483
  pilB Vibrio campbellii strain DS40M4

47.509

99.118

0.471
  pilF Thermus thermophilus HB27

38.899

99.295

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.454

93.298

0.377

Multiple sequence alignment