Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FY043_RS25845 Genome accession   NZ_CP043396
Coordinates   5475294..5476562 (-) Length   422 a.a.
NCBI ID   WP_082426354.1    Uniprot ID   -
Organism   Pseudomonas monteilii strain 170620603RE     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5470294..5481562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FY043_RS25830 (FY043_26315) - 5471947..5473560 (-) 1614 WP_164525171.1 SPOR domain-containing protein -
  FY043_RS25835 (FY043_26320) aroB 5473576..5474673 (-) 1098 WP_060498049.1 3-dehydroquinate synthase -
  FY043_RS25840 (FY043_26325) aroK 5474771..5475289 (-) 519 WP_028698445.1 shikimate kinase AroK -
  FY043_RS25845 (FY043_26330) pilQ 5475294..5476562 (-) 1269 WP_082426354.1 type IV pilus secretin PilQ Machinery gene
  FY043_RS25850 (FY043_26335) - 5476486..5477487 (-) 1002 WP_104835405.1 pilus assembly protein PilP -
  FY043_RS25855 (FY043_26340) - 5477499..5478035 (-) 537 WP_060498051.1 PilN domain-containing protein -
  FY043_RS25860 (FY043_26345) pilM 5478032..5478913 (-) 882 WP_104833799.1 type IV pilus biogenesis protein PilM -
  FY043_RS25865 (FY043_26350) - 5479105..5481558 (+) 2454 WP_164525172.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45952.68 Da        Isoelectric Point: 4.6649

>NTDB_id=383317 FY043_RS25845 WP_082426354.1 5475294..5476562(-) (pilQ) [Pseudomonas monteilii strain 170620603RE]
MKMLMKWVLAALLPIPLASAMPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVAGDDVQGNVTLRLQDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAAAFAAQPFDAPVGLEPDAQVQPLRRELIPIHHAKAAELVELLLTSLADDGTLTARGSLAVDE
RTNTLVAYQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYSENLRPGQELFVDLGVERAGSSL
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=383317 FY043_RS25845 WP_082426354.1 5475294..5476562(-) (pilQ) [Pseudomonas monteilii strain 170620603RE]
ATGAAAATGTTGATGAAATGGGTGTTGGCAGCCCTGCTGCCGATCCCGCTGGCGTCGGCGATGCCTTACCAGGGTGAGCC
CCTGTCGTTGAACTTCCAGGATGTGGAAGTGCGCTCGGTGTTGCAGGTACTGGCTGATTATGCGGGCGTCAACCTGGTTG
CGGGTGACGACGTACAAGGCAACGTCACCTTGCGTTTACAGGATGTACCTTGGGATCAGGCACTCGACCTGGTGTTGCGA
AGCAAGGGGCTGGCCCGGCGGCAGGAGGGTAATGTACTGCTGGTGGCACCTGCGGCAGCGTTTGCCGCGCAGCCGTTCGA
TGCCCCCGTGGGGCTGGAGCCGGATGCGCAGGTGCAGCCGCTGCGCCGTGAGCTGATACCGATCCATCATGCCAAAGCCG
CCGAGTTGGTTGAGCTATTGCTGACCAGCCTGGCGGATGACGGCACCCTCACTGCCAGAGGCAGCCTGGCAGTCGACGAG
CGCACCAACACACTGGTGGCGTACCAGCCCGCCGACCGCCTGGCGGAACTGCGGCAGCTGGTTGCTCAACTGGACGTGCC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATCGTTGAAGCCAACGTCGATTACGAGAAAAGCCTGGGGGTGCGCTGGGGCG
GGCCGCTGTATAGCGAAAACCTGCGGCCGGGCCAGGAGCTGTTTGTCGATCTTGGTGTGGAGCGCGCGGGCAGTAGTCTC
GGTCTGGGCCTGCTGCGTGGCGACGTGTTGCTCGACCTGGAGCTCAGCGCCATGGAAAAAAGCGGCAACGGCGAGATCAT
TTCGCAGCCCAAAGTGGTAACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCTTACCAGGAGACCA
GTCAGAGTGGCGCCACCTCGGTGTCGTTTCGCGAAGCCTCGCTGTCGCTGGAGGTGACTCCGCAGATCACCCCGGACAAT
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTGCCGCCGATTCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGCCGACGGTGAAACGATCGTGATTGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(50-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.174

100

0.611
  comQ Acinetobacter baylyi ADP1

42.667

100

0.455
  pilQ Acinetobacter baumannii strain A118

41.048

100

0.445
  pilQ Acinetobacter baumannii D1279779

41.048

100

0.445
  pilQ Vibrio campbellii strain DS40M4

40.323

100

0.415
  pilQ Legionella pneumophila strain Lp02

41.627

99.052

0.412
  pilQ Legionella pneumophila strain ERS1305867

41.627

99.052

0.412
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.093

99.763

0.41
  pilQ Vibrio cholerae strain A1552

41.093

99.763

0.41

Multiple sequence alignment