Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FXN65_RS25795 Genome accession   NZ_CP043311
Coordinates   5604962..5607070 (-) Length   702 a.a.
NCBI ID   WP_151137783.1    Uniprot ID   -
Organism   Metapseudomonas lalkuanensis strain PE08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5599962..5612070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXN65_RS25780 (FXN65_25780) - 5601624..5603273 (-) 1650 WP_151137774.1 SPOR domain-containing protein -
  FXN65_RS25785 (FXN65_25785) aroB 5603283..5604386 (-) 1104 WP_151137777.1 3-dehydroquinate synthase -
  FXN65_RS25790 (FXN65_25790) aroK 5604438..5604956 (-) 519 WP_151137780.1 shikimate kinase AroK -
  FXN65_RS25795 (FXN65_25795) pilQ 5604962..5607070 (-) 2109 WP_151137783.1 type IV pilus secretin PilQ Machinery gene
  FXN65_RS25800 (FXN65_25800) pilP 5607128..5607661 (-) 534 WP_151137785.1 type 4a pilus biogenesis lipoprotein PilP -
  FXN65_RS25805 (FXN65_25805) pilO 5607658..5608281 (-) 624 WP_151137788.1 type 4a pilus biogenesis protein PilO -
  FXN65_RS25810 (FXN65_25810) - 5608278..5608877 (-) 600 WP_151137791.1 PilN domain-containing protein -
  FXN65_RS25815 (FXN65_25815) pilM 5608877..5609941 (-) 1065 WP_151137793.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 702 a.a.        Molecular weight: 75381.60 Da        Isoelectric Point: 4.8826

>NTDB_id=382811 FXN65_RS25795 WP_151137783.1 5604962..5607070(-) (pilQ) [Metapseudomonas lalkuanensis strain PE08]
MKSSLSRLGITLLAALMSPALLAANLQGLDVAALPGDRVELKLAFDEPVVAPRGYTIEQPARIALDLPGVSNKLGSKNRE
LGVGNARSVTVVEAKDRTRLIINLSTLVPYSTRVDGRNLYVLVGDSASAPAAPRQVTAAPAPAPAAAQLAASQPVKSYAR
AGRSIENIDFQRGDQGEGNVVITLSDPSVSPDIQEQGGKIRLAFPKTQLPEPLRVRLDVKDFATPVQFVSASGAGNDASI
SIEPTGYYDYLAYQTDNKLTVSIKPLTQEDVEKRKTERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQS
VTSGDPSAAGADERGSITVDDRTNSIIAYQTEDKLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVNWRGASIGD
NNFVVGGGGLGRPAANQAGNVNLANAPFVDLGADSATSSIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETA
KILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFANALNGVPPINKNEVNAKVLVNDGE
TIVIGGVFSNTQTKAVDKVPFLGDLPFLGRMFRRDVVQDKKAELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2109 bp        

>NTDB_id=382811 FXN65_RS25795 WP_151137783.1 5604962..5607070(-) (pilQ) [Metapseudomonas lalkuanensis strain PE08]
ATGAAAAGCTCGCTATCGCGCCTTGGTATCACGCTATTGGCCGCCCTGATGTCACCTGCGCTGCTGGCGGCGAACCTGCA
GGGGCTGGATGTTGCGGCCCTGCCGGGCGACAGGGTCGAACTCAAACTGGCCTTCGACGAGCCGGTGGTAGCTCCTCGCG
GCTACACCATCGAGCAGCCAGCACGCATTGCTCTGGACCTGCCGGGCGTGTCCAACAAGCTGGGTTCGAAGAATCGGGAG
CTGGGCGTGGGCAATGCCCGCAGCGTGACCGTGGTGGAAGCCAAGGACCGCACTCGCCTGATCATCAATCTGTCCACGCT
CGTGCCCTACAGTACCCGCGTAGATGGCCGAAACCTCTATGTGCTGGTGGGGGACTCCGCATCTGCGCCGGCAGCCCCGC
GTCAGGTTACTGCGGCCCCGGCGCCCGCCCCGGCCGCCGCACAACTGGCCGCCTCGCAGCCGGTCAAGAGCTACGCCCGT
GCCGGTCGGAGCATCGAAAACATCGACTTCCAGCGTGGTGATCAGGGTGAGGGCAATGTGGTGATCACCTTGTCCGATCC
CAGCGTCAGCCCGGACATCCAGGAGCAAGGCGGCAAGATCCGTCTGGCCTTCCCCAAGACCCAGTTGCCTGAGCCGCTGC
GCGTTCGCCTGGACGTTAAGGACTTCGCTACGCCGGTGCAATTCGTAAGCGCGTCGGGTGCCGGCAACGACGCCAGCATC
AGCATCGAACCCACCGGCTATTACGACTACCTGGCCTATCAGACCGACAACAAGCTGACCGTCAGCATCAAGCCGCTGAC
GCAGGAAGATGTGGAGAAGCGCAAGACCGAGCGTTTCGCCTATTCCGGCGAGAAGCTGTCGTTGAACTTCCAGGACATCG
ATGTGCGCTCGGTGCTGCAACTGATCGCCGACTTCACCGACCTCAACCTGGTGGCCTCGGATACCGTACAAGGCAACATC
ACCCTGCGCCTGCAGAACGTGCCCTGGGACCAGGCACTGGATCTGGTGCTGAAGACCAAAGGCCTGGACAAGCGCAAGGT
AGGTAACGTGCTGCTGGTCGCGCCGGCTGATGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAGTCGCAGAAGCAGATTG
CCGAACTGGCCCCTCTGCGCCGCGAGCTGATCCAGGTGAACTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAATCC
GTCACCAGCGGGGACCCGAGCGCTGCCGGTGCCGATGAGCGCGGTTCCATCACGGTAGACGACCGTACCAACAGCATCAT
CGCCTACCAGACCGAAGATAAGCTGGATGAGCTGCGTCGCATCGTTACCCAGCTGGATATCCCGGTTCGCCAGGTAATGA
TCGAAGCGCGTATCGTCGAAGCCAACGTCGATTACGACAAGGCCCTGGGCGTGAACTGGCGTGGTGCGAGCATCGGCGAC
AACAACTTCGTCGTTGGTGGCGGTGGTCTCGGTCGTCCGGCCGCCAACCAGGCGGGCAATGTCAACCTGGCCAACGCCCC
CTTCGTCGATCTCGGCGCTGACAGCGCGACGTCCAGCATCGGCATCGGCTTCATTACCGACAACACCATCCTGGACCTCG
AGCTGTCGGCGATGGAGAAGACCGGTAACGGCGAGATCGTATCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCC
AAGATCCTCAAAGGCCAGGAAGTGCCCTACCAGGAAGCCAGCTCCAGCGGTGCCACCAGCACCTCGTTCAAGGAGGCGGC
CCTGTCCCTGGAAGTCACTCCCCAGATCACGCCTGACAACCGCATCATCATGGAAGTGAAGGTGACCAAGGATGCGCCGG
ACTTCGCCAACGCCCTGAACGGAGTACCGCCGATCAACAAGAACGAGGTCAACGCCAAGGTGCTGGTCAATGATGGTGAA
ACCATTGTCATAGGTGGGGTATTCTCCAACACCCAAACCAAGGCGGTGGACAAGGTGCCCTTCCTCGGCGACCTTCCCTT
CCTCGGCCGCATGTTCCGTCGCGACGTGGTTCAGGACAAGAAAGCCGAGCTCCTGGTATTCCTCACTCCCCGCATCATGA
ACAATCAGGCCATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(537-693)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-451)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.222

100

0.792
  comQ Acinetobacter baylyi ADP1

42.466

100

0.442
  pilQ Acinetobacter baumannii strain A118

41.071

100

0.426
  pilQ Acinetobacter baumannii D1279779

41.071

100

0.426
  pilQ Legionella pneumophila strain Lp02

38.743

97.436

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.596

97.436

0.376

Multiple sequence alignment