Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FXN65_RS25815 Genome accession   NZ_CP043311
Coordinates   5608877..5609941 (-) Length   354 a.a.
NCBI ID   WP_151137793.1    Uniprot ID   -
Organism   Metapseudomonas lalkuanensis strain PE08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5603877..5614941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXN65_RS25790 (FXN65_25790) aroK 5604438..5604956 (-) 519 WP_151137780.1 shikimate kinase AroK -
  FXN65_RS25795 (FXN65_25795) pilQ 5604962..5607070 (-) 2109 WP_151137783.1 type IV pilus secretin PilQ Machinery gene
  FXN65_RS25800 (FXN65_25800) pilP 5607128..5607661 (-) 534 WP_151137785.1 type 4a pilus biogenesis lipoprotein PilP -
  FXN65_RS25805 (FXN65_25805) pilO 5607658..5608281 (-) 624 WP_151137788.1 type 4a pilus biogenesis protein PilO -
  FXN65_RS25810 (FXN65_25810) - 5608278..5608877 (-) 600 WP_151137791.1 PilN domain-containing protein -
  FXN65_RS25815 (FXN65_25815) pilM 5608877..5609941 (-) 1065 WP_151137793.1 pilus assembly protein PilM Machinery gene
  FXN65_RS25820 (FXN65_25820) - 5610123..5612561 (+) 2439 WP_151137796.1 penicillin-binding protein 1A -
  FXN65_RS25825 (FXN65_25825) - 5612623..5613891 (-) 1269 WP_151137799.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37819.08 Da        Isoelectric Point: 4.4526

>NTDB_id=382812 FXN65_RS25815 WP_151137793.1 5608877..5609941(-) (pilM) [Metapseudomonas lalkuanensis strain PE08]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGSRFKVESYAVEPLPPNAVVEKNIAELEGVGQALSRVISKARTGVKAAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVDVLLAACRKENVEVREAALAL
AGLSAKVVDVEAYALERAYSLLSAQLGDAHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=382812 FXN65_RS25815 WP_151137793.1 5608877..5609941(-) (pilM) [Metapseudomonas lalkuanensis strain PE08]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATTCGCTGTTGGGGATCGACATCAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTTCAAAGTGGAGTCCTATGCCGTCGAGCCGCTCCCACCAAACGCGGTTGTCGAAAAGAACA
TCGCGGAGCTGGAGGGCGTCGGGCAGGCACTTTCCCGAGTAATCAGCAAGGCGCGTACCGGCGTCAAGGCCGCGGCAGTG
GCTGTGGCCGGTTCGGCCGTGATCACCAAGACCATCGAAATGGAAGCCGGGCTCTCCGAAGACGATCTGGAGAACCAGCT
GAAGATCGAGGCCGACCAGTACATTCCCTATCCGCTGGAAGAAGTCGCCATCGACTTCGAGGTGCAAGGTGCCTCGGCAC
GCAACCCGGAGCGGGTCGATGTGCTGCTCGCAGCCTGCCGAAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTCGCCCTG
GCCGGACTTTCCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCGCCTATTCGCTGCTTTCGGCACAGCTGGG
CGATGCTCATGACGAGCTGACCGTCGCAGTCGTCGACATTGGCGCCACCATGACGACCCTCAGCGTGCTGCACAACGGCC
GCACCATCTATACCCGCGAACAGCTCTTCGGTGGTCGTCAACTGACCGAGGAGATCCAGCGTCGCTATGGTCTTTCCGTT
GAAGAAGCTGGTCTCGCCAAGAAACAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTTCTGCAACCGTTCAAGGATGC
TGTAGTCCAGCAGGTTTCCCGTTCGCTGCAGTTCTTCTTCGCAGCCGGCCAGTTCAATGATGTGGATTACATCCTCCTGG
CAGGCGGCACGGCTTCGATCCCGGATCTCGATCGCCTGATCCAGCAGAAGATCGGTACACCGACCTTGGTTGCCAATCCC
TTCGCCGATATGGCGCTGAGCAGCAAGGTCAATGCTGGTGCGCTGGCTAGCGATGCGCCTGCCCTGATGATTGCGTGTGG
CTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

52.825

100

0.528
  pilM Legionella pneumophila strain ERS1305867

46.045

100

0.46

Multiple sequence alignment