Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FYN22_RS14640 Genome accession   NZ_CP043307
Coordinates   3016327..3018039 (+) Length   570 a.a.
NCBI ID   WP_148826861.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain Acsw19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3011327..3023039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYN22_RS14595 (FYN22_14595) nusA 3011912..3013396 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  FYN22_RS14600 (FYN22_14600) rimP 3013436..3013960 (-) 525 WP_148826860.1 ribosome maturation factor RimP -
  FYN22_RS14630 (FYN22_14630) secG 3014913..3015242 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  FYN22_RS14635 (FYN22_14635) tpiA 3015256..3016047 (-) 792 WP_010325769.1 triose-phosphate isomerase -
  FYN22_RS14640 (FYN22_14640) pilB 3016327..3018039 (+) 1713 WP_148826861.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FYN22_RS14645 (FYN22_14645) pilC 3018070..3019296 (+) 1227 WP_010325767.1 type II secretion system F family protein Machinery gene
  FYN22_RS14650 (FYN22_14650) pilD 3019296..3020156 (+) 861 WP_148826862.1 prepilin peptidase Machinery gene
  FYN22_RS14655 (FYN22_14655) coaE 3020163..3020762 (+) 600 WP_148826863.1 dephospho-CoA kinase -
  FYN22_RS14660 (FYN22_14660) - 3020776..3021693 (-) 918 WP_148826864.1 DMT family transporter -
  FYN22_RS14665 (FYN22_14665) rlmB 3021726..3022475 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FYN22_RS14670 (FYN22_14670) - 3022618..3022941 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63551.91 Da        Isoelectric Point: 5.0102

>NTDB_id=382777 FYN22_RS14640 WP_148826861.1 3016327..3018039(+) (pilB) [Acinetobacter johnsonii strain Acsw19]
MSVSNGLPKFTGFIRRLVEDGRVTAKNMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEEDPQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=382777 FYN22_RS14640 WP_148826861.1 3016327..3018039(+) (pilB) [Acinetobacter johnsonii strain Acsw19]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTAAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAACCATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATTCTCTATAT
CGCTACCAGCAATCCAACCAACATGGATGCGATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCCCAGCTTGAAAAACTGATCGAACAAAATTTTGCGCAGGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAAGAAGACCCTCAGAACTCCAATGACGAAGAAGACGATTCACCTCAAGGCGATGAAGCACCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGATTTACACTTTGAGCCTTATGAGAAATCTT
ACCGTGTCCGTTATCGTGTAGATGGGGTTCTGCGTCACATTGCCAGCCCACCACTACAACTGGCGACTCGTCTTGCTTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGTCGTATTAAATTAAAGCTGTCAAA
AAGTAAAGCCATTGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGTGAAAAATTGGTTCTGCGTATTCTCGATCCAT
CTAGTGCTATGCTAGGGATCGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTGTTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTGTCGTTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCAGAAGACCCCGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAACGTGAACAATA
AAGTCGGTCTTACCTTCTCTGCTGCGCTGAAGTCATTCTTACGTCAAGATCCTGATATTGTGATGGTGGGTGAGATTCGT
GACCTTGAAACAGCGGAAATCGCGATTAAAGCTGCGCAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGAAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTAATTGCAC
AACGTTTAGCACGCCGCTTATGTAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGTTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGATGTACTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.404

100

0.814
  pilB Acinetobacter baylyi ADP1

77.018

100

0.77
  pilB Legionella pneumophila strain ERS1305867

53.471

93.509

0.5
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.925

97.895

0.479
  pilB Vibrio campbellii strain DS40M4

48.307

98.421

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

50.775

90.526

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.572

100

0.375
  pilF Thermus thermophilus HB27

36.427

100

0.365

Multiple sequence alignment