Detailed information NTG0382748

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FYN22_RS01245
• Genome accession: NZ_CP043307
• Coordinates: 277087..278145 (+)
• Length: 352 a.a.
• NCBI ID: WP_058870420.1
• Uniprot ID: A0A345B4L2
• Organism: Acinetobacter johnsonii strain Acsw19
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 272087..283145

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FYN22_RS01230 (FYN22_01230)	mraY	272366..273484 (+)	1119	WP_004691502.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
FYN22_RS01235 (FYN22_01235)	-	273555..274361 (+)	807	WP_148825382.1	putative RNA methyltransferase	-
FYN22_RS01240 (FYN22_01240)	ponA	274393..276921 (-)	2529	WP_148825383.1	penicillin-binding protein PBP1a	-
FYN22_RS01245 (FYN22_01245)	pilM	277087..278145 (+)	1059	WP_058870420.1	pilus assembly protein PilM	Machinery gene
FYN22_RS01250 (FYN22_01250)	pilN	278145..278801 (+)	657	WP_148825384.1	PilN domain-containing protein	Machinery gene
FYN22_RS01255 (FYN22_01255)	pilO	278798..279520 (+)	723	WP_148825385.1	type 4a pilus biogenesis protein PilO	Machinery gene
FYN22_RS01260 (FYN22_01260)	pilP	279520..280047 (+)	528	WP_094148569.1	pilus assembly protein PilP	Machinery gene
FYN22_RS01265 (FYN22_01265)	pilQ	280067..282145 (+)	2079	WP_148825386.1	type IV pilus secretin PilQ	Machinery gene
FYN22_RS01270 (FYN22_01270)	-	282234..282716 (+)	483	WP_249303512.1	shikimate kinase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38953.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=382748 FYN22_RS01245 WP_058870420.1 277087..278145(+) (pilM) [Acinetobacter johnsonii strain Acsw19]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARSEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=382748 FYN22_RS01245 WP_058870420.1 277087..278145(+) (pilM) [Acinetobacter johnsonii strain Acsw19]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAATAATCGGTATTGGGTGGAGAGCTATGCACTAGTCCCATTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCAGTTGGTGATGCCCTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACGTCCATGGTGATTACTAAAATCATCGAAATGGATGCCGATATGAACGATGACGAACGTGAAATCCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTGGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCAAATCGTGTCAATGTACTCCTCGTTGCAACCCGCATTGAAAATGTAGAGGCCCGATCCGAAGCATTGGAGTTA
GGTGGTTTAACACCTAAGATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTCTTTTCAGATACGCTTCC
AATGGGCGTAAATACTGTTGGGATTCTGGACATCGGTCACACCATGACGACCTTATCCGTCATGCAAAACAATAAAGTCA
TTTATACCCGTGAACAGGTGTTCGGGGGTAAACAACTGACGCAAGAAATTCAAAACCGTTATGGCTTGTCTTTTGAAGAA
GCAGGACGCGCCAAAAAAAGCCGTACCCTGCCTGACGACTATGATATTGAAGTGCTTGAGCCTTTTCTTGAAGCTGTGGT
GCAACAAGCAGCACGCTCTTTGCAGTTCTTCTTTTCGTCCTCTCAATTCAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTGGCGAAGTTATTACAACAAAAATTAGGTTATCGGGTCACCATTGCCAACCCATTTTTA
CAAATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A345B4L2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412