Detailed information NTG0380274

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FXO11_RS03040
• Genome accession: NZ_CP043042
• Coordinates: 699072..700193 (+)
• Length: 373 a.a.
• NCBI ID: WP_148861520.1
• Uniprot ID: -
• Organism: Marinobacter fonticola strain CS412
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 694072..705193

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FXO11_RS03020	-	695284..695868 (-)	585	WP_148861516.1	YggT family protein	-
FXO11_RS03025	proC	695942..696772 (-)	831	WP_148861517.1	pyrroline-5-carboxylate reductase	-
FXO11_RS03030	-	696887..697585 (-)	699	WP_148861518.1	YggS family pyridoxal phosphate-dependent enzyme	-
FXO11_RS03035	pilT	697855..698889 (+)	1035	WP_148861519.1	type IV pilus twitching motility protein PilT	Machinery gene
FXO11_RS03040	pilU	699072..700193 (+)	1122	WP_148861520.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FXO11_RS03045	-	700427..701830 (+)	1404	WP_148861521.1	OmpP1/FadL family transporter	-
FXO11_RS03050	-	702552..704345 (+)	1794	WP_148861522.1	TonB-dependent receptor domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41465.63 Da
• Isoelectric Point: 6.6636

>NTDB_id=380274 FXO11_RS03040 WP_148861520.1 699072..700193(+) (pilU) [Marinobacter fonticola strain CS412]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRAEFDETHESNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQLDDLGLPDIIKDLAMTKRGLIIFVGATGTGKSTSLAAMIGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTENFEVALKNTLRQAPDVILIGEVRTKQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFNPDQHNQIWMDLSLNLRAVVAQQLVPTPDGKGRRAVIEVLINTPLVQDLIRKGEVHKLKELMAKSTEMGMQTFDQA
LYRLYAEGAITYEDALAHADSANDLRLMIKLGADAAGSDKLSSTVDKLSIQDD

Nucleotide

• Length: 1122 bp

>NTDB_id=380274 FXO11_RS03040 WP_148861520.1 699072..700193(+) (pilU) [Marinobacter fonticola strain CS412]
ATGGAATTTGAGAAACTGCTGCGGTTGATGGTGGAGAAAGGGGGCTCAGACCTCTTCATTACGGCCGGTGTGCCGCCCAG
CATGAAGGTGAACGGTAAAGTGTTGCCGGTTACCAAGAACGCGCTTACGCCGGAGCAGACCCGGGAATTCGTCTACGGGG
CGATGAACGATAAGCAGCGCGCCGAATTCGACGAAACCCACGAGAGCAACTTCGCGATCAGCGCCCGGGGCATTGGCCGG
TTCCGTGTCAGCGCCTTCTTCCAGCGCAACCTGTGCGGCATGGTGCTACGCCGTATCGAGGTGAAGATTCCGCAGCTCGA
CGATCTGGGACTGCCCGATATCATCAAGGATTTGGCCATGACCAAGCGTGGCCTGATTATTTTCGTCGGCGCCACCGGAA
CCGGTAAGTCCACGTCGCTGGCGGCCATGATCGGCCACCGCAATCGCAACAGCCGTGGGCACATCATCTCGATCGAGGAC
CCGATTGAATTCGTCCACCAGCATCAGGGATGTATCGTGACCCAGCGTGAAGTGGGCATCGATACAGAGAACTTCGAGGT
GGCGCTGAAAAACACGTTACGCCAAGCGCCCGATGTGATCTTGATCGGCGAGGTGCGAACCAAGCAAACCATGGAGTATT
CCGTCCAGTTCGCCGAGACCGGCCACCTGTGTCTGGCCACCCTGCACGCGAACAACGCCAACCAGGCGCTGGATCGGATT
ATCCAGTTCTTCAACCCGGACCAGCACAACCAGATCTGGATGGACCTTTCTCTCAATCTCAGGGCTGTCGTCGCCCAGCA
GTTGGTACCGACGCCGGACGGCAAGGGCCGTCGCGCGGTTATCGAAGTGCTGATCAACACGCCGCTGGTCCAGGATCTGA
TTCGAAAGGGGGAAGTTCACAAGCTCAAAGAATTGATGGCCAAATCGACCGAGATGGGCATGCAGACCTTTGACCAGGCG
CTCTACCGGCTCTACGCTGAAGGTGCGATCACCTACGAAGATGCGCTGGCCCATGCGGATTCGGCCAACGACCTGCGTCT
GATGATCAAACTGGGTGCCGATGCGGCCGGTTCTGATAAGCTTTCATCCACGGTCGACAAACTGTCGATCCAGGACGATT
AG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	78.437	99.464	0.78
pilU	Acinetobacter baylyi ADP1	65.449	95.442	0.625
pilU	Vibrio cholerae strain A1552	56.319	97.587	0.55
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.292	95.442	0.394
pilT	Acinetobacter baumannii D1279779	40.597	89.812	0.365
pilT	Acinetobacter baumannii strain A118	40.597	89.812	0.365
pilT	Acinetobacter nosocomialis M2	40.299	89.812	0.362