Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FR773_RS18585 Genome accession   NZ_CP042505
Coordinates   3917602..3918579 (-) Length   325 a.a.
NCBI ID   WP_155167280.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain E1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3912602..3923579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR773_RS18555 (FR773_18560) endA 3913188..3913895 (+) 708 WP_032613924.1 deoxyribonuclease I -
  FR773_RS18560 (FR773_18565) rsmE 3914007..3914738 (+) 732 WP_032613955.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FR773_RS18565 (FR773_18570) gshB 3914758..3915705 (+) 948 WP_032613926.1 glutathione synthase -
  FR773_RS18570 (FR773_18575) - 3915797..3916357 (+) 561 WP_032613928.1 YqgE/AlgH family protein -
  FR773_RS18575 (FR773_18580) ruvX 3916357..3916773 (+) 417 WP_130588229.1 Holliday junction resolvase RuvX -
  FR773_RS18580 (FR773_18585) - 3916813..3917502 (-) 690 WP_032613957.1 hypothetical protein -
  FR773_RS18585 (FR773_18590) pilT 3917602..3918579 (-) 978 WP_155167280.1 type IV pilus twitching motility protein PilT Machinery gene
  FR773_RS18590 (FR773_18595) - 3918597..3919298 (+) 702 WP_032613935.1 YggS family pyridoxal phosphate-dependent enzyme -
  FR773_RS18595 (FR773_18600) - 3919320..3919886 (+) 567 WP_032613937.1 YggT family protein -
  FR773_RS18600 (FR773_18605) yggU 3919883..3920179 (+) 297 WP_032613939.1 DUF167 family protein YggU -
  FR773_RS18605 (FR773_18610) - 3920183..3920776 (+) 594 WP_059307228.1 XTP/dITP diphosphatase -
  FR773_RS18610 (FR773_18615) hemW 3920769..3921911 (+) 1143 WP_155167282.1 radical SAM family heme chaperone HemW -
  FR773_RS18615 (FR773_18620) - 3921972..3922334 (+) 363 WP_103792619.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35613.78 Da        Isoelectric Point: 6.7691

>NTDB_id=378182 FR773_RS18585 WP_155167280.1 3917602..3918579(-) (pilT) [Leclercia adecarboxylata strain E1]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRQGKMELAPFPGPDVVVLLKSWLSDEQQGAWCAQGQVDFAVTLESHRLRAS
AFAHTQGCSLALRLLPEQCPQLHSLGTPRAIPELLTRDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNKQGRVALFELLINTPAAANLIREGKTYQLPGVIQTGLQAGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=378182 FR773_RS18585 WP_155167280.1 3917602..3918579(-) (pilT) [Leclercia adecarboxylata strain E1]
ATGGATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACAAGGAAAGATGGAATTGGCGCCTTTTCCGGGGCCGGATGTGGTGGTGCTGCTGAAAAGCTGGCTCAGCG
ATGAACAACAGGGGGCATGGTGTGCTCAGGGGCAGGTCGATTTTGCCGTCACTCTGGAGAGCCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCAGAACAGTGCCCGCAATTGCATAGTCTGGGTAC
GCCGCGGGCGATCCCCGAGCTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGGGCCACCGGCAGCGGCAAGTCCA
CCACGCTTGCGGCAATGGTCGACTTTCTCAATCACCACAGTGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGCAACGCCTTTGCCGATGCGCTGCGGGC
GGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAACTGCGCGATAGCGAAACGATCCGCCTTGCGCTTACCGCCG
CAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACTCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAACTGGCCGGCAGTTTGCAGGCGGTGCTGGCGCAAAAACTCGAAAGGGA
CAACAAGCAAGGGCGCGTCGCGCTGTTTGAGTTACTCATTAACACCCCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGCGTGATCCAGACCGGGCTGCAGGCAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.492
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.492
  pilT Legionella pneumophila strain Lp02

48.447

99.077

0.48
  pilT Legionella pneumophila strain ERS1305867

48.447

99.077

0.48
  pilT Acinetobacter baylyi ADP1

47.095

100

0.474
  pilT Neisseria meningitidis 8013

46.951

100

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.58

97.538

0.474
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.471
  pilT Pseudomonas aeruginosa PAK

47.95

97.538

0.468
  pilT Acinetobacter nosocomialis M2

47.649

98.154

0.468
  pilT Acinetobacter baumannii strain A118

47.649

98.154

0.468
  pilT Acinetobacter baumannii D1279779

47.649

98.154

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.631

96.615

0.422
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.385
  pilU Acinetobacter baylyi ADP1

36.533

99.385

0.363

Multiple sequence alignment