Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FR729_RS10800 Genome accession   NZ_CP042449
Coordinates   2321146..2322831 (+) Length   561 a.a.
NCBI ID   WP_167415438.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FA2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2316146..2327831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR729_RS10780 (FR729_11115) pdhR 2317722..2318489 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FR729_RS10785 (FR729_11120) ampD 2318895..2319446 (-) 552 WP_167415436.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FR729_RS10790 (FR729_11125) nadC 2319539..2320426 (+) 888 WP_017820396.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FR729_RS10795 (FR729_11130) pilA 2320688..2321146 (+) 459 WP_167415437.1 pilin Machinery gene
  FR729_RS10800 (FR729_11135) pilB 2321146..2322831 (+) 1686 WP_167415438.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FR729_RS10805 (FR729_11140) pilC 2322855..2324078 (+) 1224 WP_167415439.1 type II secretion system F family protein Machinery gene
  FR729_RS10810 (FR729_11145) pilD 2324143..2325012 (+) 870 WP_025768182.1 prepilin peptidase Machinery gene
  FR729_RS10815 (FR729_11150) coaE 2325013..2325627 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  FR729_RS10820 (FR729_11155) zapD 2325655..2326395 (+) 741 WP_005373086.1 cell division protein ZapD -
  FR729_RS10825 (FR729_11160) yacG 2326554..2326748 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  FR729_RS10830 (FR729_11165) rplS 2327232..2327585 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62133.94 Da        Isoelectric Point: 5.6871

>NTDB_id=377938 FR729_RS10800 WP_167415438.1 2321146..2322831(+) (pilB) [Vibrio alginolyticus strain FA2]
MLTNLSTALRQKRLLTFSQEESLIEQVKASGISMPEALLSSGLFTSSELAEHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTSSTLLLAVADPTNQQAEDDFRFATGLQVELVLGDFSQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKKSQELTAQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=377938 FR729_RS10800 WP_167415438.1 2321146..2322831(+) (pilB) [Vibrio alginolyticus strain FA2]
ATGCTAACCAACCTATCCACGGCTCTTCGCCAAAAAAGGTTACTGACGTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTTACATCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTATGCTTCGCTTTGTCAGCAACTTGGCCTTAGGGAA
TTAATCACGCGGCATAACGCACTCCCACTCCAGCGTACGTCTTCAACGTTATTACTCGCCGTTGCCGACCCCACCAACCA
ACAAGCAGAAGATGATTTTCGCTTTGCCACTGGTTTACAGGTTGAATTGGTCTTGGGGGATTTTAGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTATTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCCTATGAAAAGATGTACCGTGTTCGCC
TACGCTGTGATGGCATCTTAATCGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTGCCTACTCTATTTGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGTCCAACCGGGAGCGGAAAAACCGTTTCTTTGTACACCGGGCTGAGTATCCTCAATAAACCAGAAATCAA
CATTTCTACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCACTGCGCTCTTTCTTACGTCAAGACCCCGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACT
GCAGAAATCGCGATCAAAGCCTCGCAAACTGGTCACTTAGTACTTTCAACCTTGCATACCAACTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCCTCTCTTAGTTTGATTATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAAAAATCACAAGAGCTTACCGCTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGTAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTCTCCGAAGCGCTTATTAAAGGCGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.009

100

0.95
  pilB Vibrio campbellii strain DS40M4

89.661

100

0.897
  pilB Vibrio cholerae strain A1552

74.377

100

0.745
  pilB Acinetobacter baumannii D1279779

51.619

93.583

0.483
  pilB Legionella pneumophila strain ERS1305867

48.387

99.465

0.481
  pilB Acinetobacter baylyi ADP1

50.29

92.157

0.463
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

37.394

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.024

98.217

0.364

Multiple sequence alignment